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[181955_at]

Description K04B12.3 /REP_DB=WormBase Gene ID /WP=CE16226 /TR=O62301 /GB=CAB05756.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 87      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 82      6grp_total > 96      4grp_total > 05      6grp_highest_sub > Cu      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 352.3
(-1.1)
P 		315.7
(-3)
P 		331.5
(-2.2)
P 		430.5
(3)
P 		115
B 347.5
(0.7)
P 		253.4
(-3)
P 		270.3
(-2.3)
P 		408
(3)
P 		155
C 336.3
(0.6)
P 		254.6
(-3)
P 		284.7
(-1.7)
P 		390
(3)
P 		135
avr 345.4
(0.1) 		274.6
(-3) 		295.5
(-2.1) 		409.5
(3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 385.2
(0.9)
P 		298.7
(-2.2)
P 		275.7
(-3)
P 		367.1
(0.3)
P 		399.1
(1.4)
P 		443.9
(3)
P 		168
B 360.2
(-0.7)
P 		276.2
(-2.7)
P 		264.1
(-3)
P 		400.8
(0.2)
P 		387.9
(-0.1)
P 		517.7
(3)
P 		254
C 334.3
(-3)
P 		337.9
(-2.8)
P 		358.5
(-1.5)
P 		363.7
(-1.2)
P 		385.9
(0.1)
P 		433.4
(3)
P 		99
avr 359.9
(-0.8) 		304.3
(-2.8) 		299.4
(-3) 		377.2
(-0.2) 		391
(0.3) 		465
(3) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 128.3
(3)
P 		68.5
(-2.5)
P 		86.7
(-0.8)
P 		63
(-3)
P 		65
B 110.8
(3)
P 		62.6
(-3)
P 		62.6
(-3)
P 		86.9
(0)
P 		48
C 111.3
(3)
P 		73.2
(-2)
P 		99.2
(1.4)
P 		65.8
(-3)
P 		46
avr 116.8
(3) 		68.1
(-3) 		82.8
(-1.2) 		71.9
(-2.5) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 71
(-1.9)
P 		106.2
(1.6)
P 		60
(-3)
P 		93.1
(0.3)
P 		120.4
(3)
P 		108.1
(1.8)
P 		60
B 125.3
(-0.2)
P 		91.9
(-1.7)
P 		64.1
(-3)
P 		106.2
(-1)
P 		157.6
(1.3)
P 		193.1
(3)
P 		129
C 123.9
(1.2)
P 		103.1
(-0.5)
P 		91.2
(-1.5)
P 		73.1
(-3)
P 		124.9
(1.3)
P 		145.1
(3)
P 		72
avr 106.7
(-0.3) 		100.4
(-0.8) 		71.8
(-3) 		90.8
(-1.5) 		134.3
(1.9) 		148.8
(3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181955_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3451
Sequence Type Exemplar sequence
Representative Public ID K04B12.3 NCBI
Target Description K04B12.3 /REP_DB=WormBase Gene ID /WP=CE16226 /TR=O62301 /GB=CAB05756.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064602(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064602 NCBI Caenorhabditis elegans K04B12.3 (K04B12.3) mRNA, complete cds. 11/11 None
SNAP00000010722 ENSEMBL cdna:SNAP chromosome:CEL140:II:14441330:14447032:1 11/11 None
GENEFINDER00000010723 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14441330:14447028:1 11/11 None
K04B12.3 ENSEMBL cdna:known chromosome:CEL140:II:14441325:14447098:1 gene:K04B12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14441326-14447029(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K04B12.3
Entrez Gene 175103 Entrez gene
SwissProt O62301 EMBL-EBI
WormBase CE37027 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05756 Hypothetical protein K04B12.3 [Caenorhabditis elegans] ref|NP_497003.2| K04B12.3 [Caenorhabditis elegans] 0.0
blast CAE57928 Hypothetical protein CBG00981 [Caenorhabditis briggsae] 0.0
blast EAA13828 ENSANGP00000012357 [Anopheles gambiae str. PEST] ref|XP_319357.2| ENSANGP00000012357 [Anopheles gambiae str. PEST] 7.0E-32
Sequence
Target Sequence 
>C. ELEGANS:181955_AT
tgaacttcgacgtctggaaccgcgatctgaacaaaattcgaagcgaggatttcagtcgaa
agtgcggaaaagacaacaaggacgactcggcacgtgtcaaactttttgttggattcgaat
atgaatgttcccgtggtcaccgcttctttgtggactacaacggagaaccgctcatctact
cgaaaggatcgaatgttatcagagaatccgcgcaccgaagttcgttgggaaatgtcctcc
aggctgatcttccgattagaagaccatgtacgtgccggaagcttccactgcaatccgcac
aactgcagaaggttcatgtggtgactccaaaagcaccagttcatattactattgatccga
aagttttgattccaactcacgaaggcgtctatggaactggacaggagccgctggaacttc
atcattcaaaatactacatcctccacctgccaactgtctactctggtccatcgggagcct
ggatgcccggagagttcaatcctgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACTTCGACGTCTGGAACCGCGA 260 573 2108 Antisense
ACTCGGCACGTGTCAAACTTTTTGT 390 99 2192 Antisense
GAATATGAATGTTCCCGTGGTCACC 619 325 2224 Antisense
TCACCGCTTCTTTGTGGACTACAAC 2 625 2244 Antisense
ATCCGCGCACCGAAGTTCGTTGGGA 472 29 2313 Antisense
TTGGGAAATGTCCTCCAGGCTGATC 156 705 2332 Antisense
GAAGACCATGTACGTGCCGGAAGCT 155 339 2366 Antisense
GATTCCAACTCACGAAGGCGTCTAT 207 433 2475 Antisense
CATTCAAAATACTACATCCTCCACC 132 215 2530 Antisense
TCCATCGGGAGCCTGGATGCCCGGA 196 579 2574 Antisense
GATGCCCGGAGAGTTCAATCCTGAA 554 413 2589 Antisense
Sequence Source Affymetrix Proprietary Database