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[181950_at]

Description C54H2.1 /REP_DB=WormBase Gene ID /WP=CE06983 /TR=Q18863 /GB=AAB00590.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 41      4grp_total > 31      6grp_cgc > 71      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 324
1 2 3 4 range
A 370.4
(3)
A 		100.9
(-3)
A 		150.8
(-1.9)
A 		338.8
(2.3)
A 		270
B 402.8
(3)
A 		171.6
(-2.1)
A 		130.6
(-3)
A 		227.4
(-0.9)
A 		272
C 424.8
(3)
A 		156.7
(-3)
A 		206.9
(-1.9)
A 		224.7
(-1.5)
A 		268
avr 399.3
(3) 		143.1
(-3) 		162.8
(-2.5) 		263.6
(-0.2) 		256

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 283
1 2 3 4 5 6 range
A 288
(0.8)
A 		309.3
(1.2)
A 		393.4
(3)
A 		331.8
(1.7)
A 		110.6
(-3)
A 		173.4
(-1.7)
A 		283
B 316.1
(3)
A 		273.5
(1.1)
A 		249.1
(-0)
A 		227.1
(-1)
A 		264.9
(0.7)
A 		183.9
(-3)
A 		132
C 224.7
(-1.1)
A 		324
(2.1)
A 		350.9
(3)
A 		326
(2.2)
A 		165.2
(-3)
A 		244.4
(-0.4)
A 		186
avr 276.3
(0.8) 		302.3
(1.9) 		331.1
(3) 		295
(1.6) 		180.2
(-3) 		200.6
(-2.2) 		151
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 134.9
(3)
A 		21.9
(-3)
A 		39.4
(-2.1)
A 		49.6
(-1.5)
A 		113
B 128.5
(3)
A 		42.4
(-2.3)
A 		30.3
(-3)
A 		48.5
(-1.9)
A 		98
C 140.6
(3)
A 		45
(-2.6)
A 		72.1
(-1)
A 		37.9
(-3)
A 		103
avr 134.7
(3) 		36.4
(-3) 		47.3
(-2.3) 		45.3
(-2.5) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 53.1
(-1.5)
A 		110
(3)
A 		85.7
(1.1)
A 		84.1
(1)
A 		33.4
(-3)
A 		42.2
(-2.3)
A 		77
B 110
(3)
A 		91
(0.7)
A 		60.5
(-3)
A 		60.2
(-3)
A 		107.6
(2.7)
A 		68.6
(-2)
A 		50
C 83.3
(0.9)
A 		98.9
(3)
A 		89.3
(1.7)
A 		65.6
(-1.4)
A 		53.5
(-3)
A 		81.8
(0.7)
A 		45
avr 82.1
(-0) 		100
(3) 		78.5
(-0.6) 		70
(-2) 		64.8
(-2.9) 		64.2
(-3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181950_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16411
Sequence Type Exemplar sequence
Representative Public ID C54H2.1 NCBI
Target Description C54H2.1 /REP_DB=WormBase Gene ID /WP=CE06983 /TR=Q18863 /GB=AAB00590.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029329(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029329 NCBI Caenorhabditis elegans SYnthetic lethal with Mec family member (sym-3) (sym-3) mRNA, complete cds. 11/11 None
SNAP00000031927 ENSEMBL cdna:SNAP chromosome:CEL140:X:5776193:5778579:1 11/11 None
GENEFINDER00000031930 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5776244:5778579:1 11/11 None
C54H2.1 ENSEMBL cdna:known chromosome:CEL140:X:5776193:5778823:1 gene:C54H2.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029330 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
183819 2 1 NM_001029329 181950_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5776192-5778579(+) 100.0 100.0
Public Domain and Genome References
Ensembl C54H2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO66457 SYM-3A [Caenorhabditis elegans] ref|NP_001024500.1| SYnthetic lethal with Mec family member (sym-3) [Caenorhabditis elegans] gb|AAX55695.1| Synthetic lethal with mec protein 3, isoform a [Caenorhabditis elegans] 1.0E-174
blast T29610 hypothetical protein C54H2.1 - Caenorhabditis elegans 1.0E-161
blast T29610 hypothetical protein C54H2.1 - Caenorhabditis elegans 1.0E-155
blast CAE68798 Hypothetical protein CBG14748 [Caenorhabditis briggsae] 1.0E-145
blast T29610 hypothetical protein C54H2.1 - Caenorhabditis elegans 1.0E-144
blast AAO66457 SYM-3A [Caenorhabditis elegans] ref|NP_001024500.1| SYnthetic lethal with Mec family member (sym-3) [Caenorhabditis elegans] gb|AAX55695.1| Synthetic lethal with mec protein 3, isoform a [Caenorhabditis elegans] 1.0E-144
blast CAE68798 Hypothetical protein CBG14748 [Caenorhabditis briggsae] 1.0E-138
Sequence
Target Sequence 
>C. ELEGANS:181950_AT
atcaacctctccgaatatgcagcttccggtgttgaaggaatctcgaggacctatctttta
aacggctacactagcaatcaaagattggataactccaaagtgtgcatcaaagttgcaatg
acacatcaaagtgcagacccattcttcagagtgcctcgcctgtcaacgttcggtccacga
caagatggagctattgatcaagatggttttcgagcagacgatgaaactgattccgaagaa
ggaacatcgtctcatccgaagctttccaatgtagatgttctagaaagttcatccgccgct
agcaactcgcaagtcgacgaaccagttgtcgaacgacgagtaatacatccaccacaacat
ccaacctgccaactacgacgatttagtcaagatcgatctgctcaaaaaattcaacactcc
agattcgatgcagacaacgtgattgacaaaatcattgccgaatgtcgaatatcggaagat
gaagtggagagtaacagcggaggcttggtgctcgagaaattcttggataaacatggaaag
ccacttgtcaatccacgtcaacccgtgaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAACCTCTCCGAATATGCAGCTT 375 27 259 Antisense
AAAGTGCAGACCCATTCTTCAGAGT 411 121 386 Antisense
TGTCAACGTTCGGTCCACGACAAGA 325 557 419 Antisense
GAACATCGTCTCATCCGAAGCTTTC 500 351 500 Antisense
TGTTCTAGAAAGTTCATCCGCCGCT 34 563 534 Antisense
GCAACTCGCAAGTCGACGAACCAGT 160 319 560 Antisense
GACGAGTAATACATCCACCACAACA 273 377 593 Antisense
CAACCTGCCAACTACGACGATTTAG 590 185 620 Antisense
TCAACACTCCAGATTCGATGCAGAC 646 629 669 Antisense
GTAACAGCGGAGGCTTGGTGCTCGA 52 463 749 Antisense
GTCAATCCACGTCAACCCGTGAAAC 698 463 805 Antisense
Sequence Source Affymetrix Proprietary Database