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[181927_s_at]

Description T22F3.8 /REP_DB=WormBase Gene ID /WP=CE13976 /TR=Q94305 /GB=AAB18311.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 47      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 163.8
(3)
A 		9.6
(-3)
A 		14.9
(-2.8)
A 		58.4
(-1.1)
A 		154
B 153.5
(3)
A 		36.9
(-2.1)
A 		15.4
(-3)
A 		19.9
(-2.8)
A 		138
C 169.2
(3)
M 		8.9
(-3)
A 		33.9
(-2.1)
A 		10.6
(-2.9)
A 		160
avr 162.2
(3) 		18.5
(-3) 		21.4
(-2.9) 		29.6
(-2.5) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 99.5
(2)
A 		49.5
(-1.3)
A 		114.6
(3)
A 		24.4
(-3)
A 		58.6
(-0.7)
A 		31.7
(-2.5)
A 		90
B 78.7
(1.5)
A 		13.1
(-2.7)
A 		102.7
(3)
A 		97.6
(2.7)
A 		33.1
(-1.4)
A 		8.1
(-3)
A 		95
C 87.4
(2.8)
A 		90.2
(3)
A 		66.6
(1.2)
A 		52.3
(0.2)
A 		28
(-1.6)
A 		9.7
(-3)
A 		81
avr 88.5
(2.5) 		50.9
(-0.4) 		94.6
(3) 		58.1
(0.2) 		39.9
(-1.2) 		16.5
(-3) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 59.6
(3)
A 		2.1
(-3)
A 		3.9
(-2.8)
A 		8.5
(-2.3)
A 		58
B 48.9
(3)
A 		9.1
(-2.3)
A 		3.6
(-3)
A 		4.2
(-2.9)
A 		45
C 56
(3)
M 		2.5
(-2.9)
A 		11.8
(-1.9)
A 		1.8
(-3)
A 		54
avr 54.8
(3) 		4.6
(-3) 		6.4
(-2.8) 		4.8
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 18.3
(0.9)
A 		17.6
(0.6)
A 		25
(3)
A 		6.2
(-3)
A 		17.7
(0.7)
A 		7.7
(-2.5)
A 		19
B 27.4
(3)
A 		4.4
(-2.7)
A 		24.9
(2.4)
A 		25.9
(2.6)
A 		13.4
(-0.4)
A 		3
(-3)
A 		24
C 32.4
(3)
A 		27.5
(2)
A 		16.9
(-0.2)
A 		10.5
(-1.5)
A 		9.1
(-1.8)
A 		3.2
(-3)
A 		29
avr 26
(3) 		16.5
(0.3) 		22.3
(2) 		14.2
(-0.3) 		13.4
(-0.5) 		4.6
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181927_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15521
Sequence Type Exemplar sequence
Representative Public ID T22F3.8 NCBI
Target Description T22F3.8 /REP_DB=WormBase Gene ID /WP=CE13976 /TR=Q94305 /GB=AAB18311.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071612(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071612 NCBI Caenorhabditis elegans T22F3.8 (T22F3.8) mRNA, complete cds. 11/11 None
SNAP00000034230 ENSEMBL cdna:SNAP chromosome:CEL140:V:3601911:3606434:-1 11/11 None
GENEFINDER00000034235 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3601911:3605240:-1 11/11 None
T22F3.8 ENSEMBL cdna:known chromosome:CEL140:V:3601911:3605240:-1 gene:T22F3.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3601910-3605240(-) 98.48 100.0
Public Domain and Genome References
Gene Title
Ensembl T22F3.8
Entrez Gene 178781 Entrez gene
SwissProt Q94305 EMBL-EBI
WormBase CE13976 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB18311 Hypothetical protein T22F3.8 [Caenorhabditis elegans] ref|NP_504013.1| T22F3.8 [Caenorhabditis elegans] 0.0
blast AAB18310 Hypothetical protein T22F3.7 [Caenorhabditis elegans] ref|NP_504012.1| T22F3.7 [Caenorhabditis elegans] 0.0
blast AAD56301 Hypothetical protein T22F3.11a [Caenorhabditis elegans] ref|NP_504015.1| T22F3.11a [Caenorhabditis elegans] 1.0E-172
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504013.1 12 15-37,44-66,97-116,123-145,150-172,184-206,216-235,278-300,315-337,349-371,414-436,443-465
T22F3.8 12 15-37,44-66,97-116,123-145,150-172,184-206,216-235,278-300,315-337,349-371,414-436,443-465
SNAP00000034230 7 89-111,121-140,183-205,220-242,254-276,319-341,348-370
GENEFINDER00000034235 12 15-37,44-66,97-116,123-145,150-172,184-206,216-235,278-300,315-337,349-371,414-436,443-465
Sequence
Target Sequence 
>C. ELEGANS:181927_S_AT
accagtccggtaatcctttgtgtctgggcaaacgcatttttcgacttgaccgctgcgata
atgttctcaacctatattccactatatttacatgaagttctgaaatttggagtcaccgag
actggattttattcaagtttaatacatggaatttgtctgccagtcaggttgtgctttggt
atcatcagtgataaaatacaattcataagtgagcctgtaaaaattcaaattttcaacaca
atatccgtcggaggttccggcctatttttcgcctcaattggcttgatcccaatggaatat
cgtggatggtcggtattctcctgcatcatgactatgagttgcattggagtgaatccggga
ggcttttataaagtcgcagtgttacattccaggcaattcgctcacgtggtgctcaccgca
atccaatggataaaatgtttggctctgtttgttgctccggcacttgtatcgatttttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCAGTCCGGTAATCCTTTGTGTCT 68 87 835 Antisense
TCTGGGCAAACGCATTTTTCGACTT 477 607 857 Antisense
TTTTCGACTTGACCGCTGCGATAAT 156 677 872 Antisense
GGAATTTGTCTGCCAGTCAGGTTGT 587 533 982 Antisense
GTCAGGTTGTGCTTTGGTATCATCA 187 467 997 Antisense
GGCCTATTTTTCGCCTCAATTGGCT 404 497 1093 Antisense
TCGTGGATGGTCGGTATTCTCCTGC 459 605 1134 Antisense
GTATTCTCCTGCATCATGACTATGA 88 453 1147 Antisense
TGTTACATTCCAGGCAATTCGCTCA 121 561 1214 Antisense
GTGCTCACCGCAATCCAATGGATAA 92 479 1243 Antisense
CTCCGGCACTTGTATCGATTTTTGT 396 235 1289 Antisense
Sequence Source Affymetrix Proprietary Database