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[181905_at]

Description C48C5.3 /REP_DB=WormBase Gene ID /WP=CE04226 /TR=Q18700 /GB=AAB37019.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 54      4grp_high_8_8 > 18      6grp_highest_nonorm > 80      6grp_total > 13      4grp_total > 22      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 209
1 2 3 4 range
A 255.2
(3)
P 		164.7
(-1.4)
P 		132.9
(-3)
P 		238.4
(2.2)
P 		122
B 239.9
(3)
P 		149
(-1.5)
P 		118.3
(-3)
P 		229.5
(2.5)
P 		122
C 307.6
(3)
P 		150
(-1.5)
P 		98.7
(-3)
P 		253.8
(1.5)
P 		209
avr 267.6
(3) 		154.6
(-1.5) 		116.6
(-3) 		240.6
(1.9) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 295.9
(3)
P 		244.8
(1.4)
P 		192.1
(-0.2)
P 		198.4
(-0)
P 		169.1
(-0.9)
P 		102.8
(-3)
P 		193
B 241.2
(3)
P 		216.8
(1.9)
P 		188.8
(0.7)
P 		155.8
(-0.7)
P 		129.5
(-1.8)
P 		102.4
(-3)
P 		139
C 167.1
(-0.5)
P 		272.8
(3)
P 		205.5
(0.8)
P 		202.6
(0.7)
P 		126.7
(-1.8)
P 		91.1
(-3)
P 		182
avr 234.7
(2.6) 		244.8
(3) 		195.5
(1) 		185.6
(0.6) 		141.8
(-1.2) 		98.8
(-3) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 92.9
(3)
P 		35.8
(-2.9)
P 		34.7
(-3)
P 		34.9
(-3)
P 		58
B 76.5
(3)
P 		36.8
(-1.9)
P 		27.4
(-3)
P 		48.9
(-0.4)
P 		49
C 101.8
(3)
P 		43.1
(-2.2)
P 		34.4
(-3)
P 		42.8
(-2.3)
P 		67
avr 90.4
(3) 		38.6
(-2.3) 		32.2
(-3) 		42.2
(-2) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 54.5
(-0.1)
P 		87.1
(3)
P 		41.8
(-1.4)
P 		50.3
(-0.6)
P 		51
(-0.5)
P 		25
(-3)
P 		62
B 83.9
(3)
P 		72.2
(1.5)
P 		45.9
(-2)
P 		41.3
(-2.6)
P 		52.6
(-1.1)
P 		38.2
(-3)
P 		46
C 61.9
(0.6)
P 		83.2
(3)
P 		52.3
(-0.5)
P 		40.7
(-1.8)
P 		41
(-1.8)
P 		30.5
(-3)
P 		53
avr 66.8
(1.3) 		80.8
(3) 		46.7
(-1.1) 		44.1
(-1.4) 		48.2
(-0.9) 		31.2
(-3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181905_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16697
Sequence Type Exemplar sequence
Representative Public ID C48C5.3 NCBI
Target Description C48C5.3 /REP_DB=WormBase Gene ID /WP=CE04226 /TR=Q18700 /GB=AAB37019.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077113(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077113 NCBI Caenorhabditis elegans C48C5.3 (C48C5.3) mRNA, complete cds. 11/11 None
SNAP00000028942 ENSEMBL cdna:SNAP chromosome:CEL140:X:8957745:8959640:-1 11/11 None
GENEFINDER00000028944 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8957745:8961101:-1 11/11 None
C48C5.3 ENSEMBL cdna:known chromosome:CEL140:X:8957745:8961068:-1 gene:C48C5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8957743-8961067(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C48C5.3
Entrez Gene 183576 Entrez gene
SwissProt Q18700 EMBL-EBI
WormBase CE04226 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37019 Hypothetical protein C48C5.3 [Caenorhabditis elegans] ref|NP_509514.1| C48C5.3 [Caenorhabditis elegans] 1.0E-166
blast AAB37019 Hypothetical protein C48C5.3 [Caenorhabditis elegans] ref|NP_509514.1| C48C5.3 [Caenorhabditis elegans] 1.0E-165
blast CAE57313 Hypothetical protein CBG00235 [Caenorhabditis briggsae] 1.0E-153
blast CAE57313 Hypothetical protein CBG00235 [Caenorhabditis briggsae] 1.0E-150
blast AAB37019 Hypothetical protein C48C5.3 [Caenorhabditis elegans] ref|NP_509514.1| C48C5.3 [Caenorhabditis elegans] 1.0E-124
blast CAE57313 Hypothetical protein CBG00235 [Caenorhabditis briggsae] 1.0E-114
blast AAK39190 Hypothetical protein C25B8.5 [Caenorhabditis elegans] ref|NP_509115.2| C25B8.5 [Caenorhabditis elegans] 4.0E-28
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509514.1 7 29-48,63-85,106-125,145-167,188-210,236-258,278-300
C48C5.3 7 29-48,63-85,106-125,145-167,188-210,236-258,278-300
SNAP00000028942 4 76-98,119-141,166-188,208-230
GENEFINDER00000028944 7 40-59,74-96,117-136,156-178,199-221,247-269,289-311
Sequence
Target Sequence 
>C. ELEGANS:181905_AT
tgccaactgggtcacttcattcaaaggtgtctcccttgctgttttcgtttggttcggatg
ctttgtctttgtgttcatttgctggtatctggaatgtttcacatgtgaagatgactgccg
gcaactaatgattcttcccaacaaagttgtaatgtatatcgtgttcacagtttctgcgtg
cattgctccaagcttaacatcacttggagtagcaatttatattttaaacgtagtgacgtc
gcatagaacaaaattatcagacgagaatgggaattcttctcacgctcttgccacccgcct
tcgaactttttatttcatatttatgaccacaatctttactgttggcacattacttcctta
ccgaatttataatattcaacgtcagctgaccccgagggaaactgatgagataagctgcgg
aagtattatcttttcatggacttgtctctactttgtctcgctcaatgcgatcctgaatcc
aataataacggtcacggttctcccacaataccgttttcaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCAACTGGGTCACTTCATTCAAA 25 587 420 Antisense
ATTCAAAGGTGTCTCCCTTGCTGTT 181 11 438 Antisense
GTTCGGATGCTTTGTCTTTGTGTTC 273 443 471 Antisense
TGCCGGCAACTAATGATTCTTCCCA 15 585 535 Antisense
TCGTGTTCACAGTTTCTGCGTGCAT 395 605 578 Antisense
TGCGTGCATTGCTCCAAGCTTAACA 658 575 594 Antisense
AGAATGGGAATTCTTCTCACGCTCT 478 73 683 Antisense
TGTTGGCACATTACTTCCTTACCGA 273 561 759 Antisense
CATGGACTTGTCTCTACTTTGTCTC 480 209 854 Antisense
ACTTTGTCTCGCTCAATGCGATCCT 277 103 869 Antisense
TTCTCCCACAATACCGTTTTCAATG 522 693 917 Antisense
Sequence Source Affymetrix Proprietary Database