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[181897_at]

Description C25H3.1 /REP_DB=WormBase Gene ID /WP=CE02499 /TR=Q18189 /GB=AAK31449.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 47      4grp_total > 28      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 52.4
(-0.8)
A 		78.6
(1.4)
A 		25.7
(-3)
A 		97.2
(3)
A 		72
B 60
(3)
A 		47.9
(0.7)
A 		28
(-3)
A 		54.2
(1.9)
A 		32
C 31.2
(-1)
A 		40.8
(-0.3)
A 		6.5
(-3)
A 		81.4
(3)
A 		75
avr 47.9
(-0.1) 		55.8
(0.7) 		20.1
(-3) 		77.6
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 36
(0.8)
A 		4.7
(-2.7)
A 		2.1
(-3)
A 		6
(-2.6)
A 		35.8
(0.7)
A 		56.1
(3)
A 		54
B 56.5
(2.9)
A 		38.5
(0.9)
A 		44
(1.5)
A 		2.1
(-3)
A 		24.6
(-0.6)
A 		57.8
(3)
A 		56
C 32.2
(-0.1)
A 		60.7
(3)
A 		34
(0.1)
A 		18
(-1.7)
A 		6.1
(-3)
A 		48.8
(1.7)
A 		55
avr 41.6
(1.3) 		34.6
(0.4) 		26.7
(-0.6) 		8.7
(-3) 		22.2
(-1.2) 		54.2
(3) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 19.1
(3)
A 		17.1
(2)
A 		6.7
(-3)
A 		14.2
(0.6)
A 		12
B 19.1
(3)
A 		11.8
(-0.5)
A 		6.5
(-3)
A 		11.6
(-0.6)
A 		13
C 10.3
(1.2)
A 		11.7
(1.9)
A 		2.3
(-3)
A 		13.7
(3)
A 		11
avr 16.2
(3) 		13.5
(1.5) 		5.2
(-3) 		13.2
(1.4) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 6.6
(-0.2)
A 		1.7
(-2.5)
A 		0.5
(-3)
A 		1.5
(-2.5)
A 		10.8
(1.7)
A 		13.7
(3)
A 		13
B 19.6
(2.4)
A 		12.8
(0.5)
A 		10.7
(-0.1)
A 		0.6
(-3)
A 		10
(-0.3)
A 		21.6
(3)
A 		21
C 11.9
(0.6)
A 		18.5
(3)
A 		8.6
(-0.6)
A 		3.6
(-2.4)
A 		2
(-3)
A 		16.3
(2.2)
A 		17
avr 12.7
(1.2) 		11
(0.6) 		6.6
(-1.2) 		1.9
(-3) 		7.6
(-0.8) 		17.2
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181897_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5130
Sequence Type Exemplar sequence
Representative Public ID C25H3.1 NCBI
Target Description C25H3.1 /REP_DB=WormBase Gene ID /WP=CE02499 /TR=Q18189 /GB=AAK31449.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062716(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062716 NCBI Caenorhabditis elegans C25H3.1 (C25H3.1) mRNA, complete cds. 11/11 None
SNAP00000016813 ENSEMBL cdna:SNAP chromosome:CEL140:II:5700137:5700867:1 11/11 None
GENEFINDER00000016820 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5700137:5701316:1 10/11 None
C25H3.1 ENSEMBL cdna:known chromosome:CEL140:II:5699800:5701375:1 gene:C25H3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5700133-5700864(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25H3.1
Entrez Gene 182922 Entrez gene
SwissProt Q18189 EMBL-EBI
WormBase CE37321 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31449 Hypothetical protein C25H3.1 [Caenorhabditis elegans] ref|NP_495117.2| C25H3.1 [Caenorhabditis elegans] 1.0E-143
blast AAK31449 Hypothetical protein C25H3.1 [Caenorhabditis elegans] ref|NP_495117.2| C25H3.1 [Caenorhabditis elegans] 1.0E-119
blast AAK31449 Hypothetical protein C25H3.1 [Caenorhabditis elegans] ref|NP_495117.2| C25H3.1 [Caenorhabditis elegans] 2.0E-84
blast CAE64559 Hypothetical protein CBG09309 [Caenorhabditis briggsae] 3.0E-39
blast CAE64559 Hypothetical protein CBG09309 [Caenorhabditis briggsae] 4.0E-39
blast CAE67695 Hypothetical protein CBG13263 [Caenorhabditis briggsae] 4.0E-39
blast CAE67695 Hypothetical protein CBG13263 [Caenorhabditis briggsae] 5.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 6.0E-19
Pfam IPR000719 EMBL-EBI Protein kinase 6.0E-19
Sequence
Target Sequence 
>C. ELEGANS:181897_AT
ctcggtggggggagctacggatgcgtccacgaggtcattgagctcaaaactggtgaccgg
tacgcaatgaaatcggagtactcctctatgaagaaacccatattactaaatgagctgaag
gtgatgaaggcaatctacacgttttccagtcaacatgttctgaaagtgagagatatggga
gttcacggaagtaccaagttcattatcatgcaaatgctcgagaagaacatggatgaagtg
ttcgagcttcttggaggttccatgactttgaacacagcagtcgcaacttcttatcagtgc
ctggaaggattggagttcatgcactgggctggatttctccatcgcgatattaagcccaat
aactactgtctggatgcgaattctgggcaaggattaagaactatttacataattgattat
ggtatatgtaaacgatttgtggacaacaataacgttatcagacaaccaaggaaaatcacc
aaatttcgaggaactctcgactttgcaccgatcgtcagtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCGGTGGGGGGAGCTACGGATGCG 626 1 85 Antisense
ATGCGTCCACGAGGTCATTGAGCTC 533 41 105 Antisense
AAACTGGTGACCGGTACGCAATGAA 544 127 131 Antisense
GGAGTACTCCTCTATGAAGAAACCC 614 511 159 Antisense
GCAATCTACACGTTTTCCAGTCAAC 565 321 214 Antisense
TGTTCGAGCTTCTTGGAGGTTCCAT 332 563 323 Antisense
AACACAGCAGTCGCAACTTCTTATC 372 135 355 Antisense
GAGTTCATGCACTGGGCTGGATTTC 245 399 397 Antisense
CTGGATTTCTCCATCGCGATATTAA 486 241 413 Antisense
AACTACTGTCTGGATGCGAATTCTG 215 141 445 Antisense
TCGACTTTGCACCGATCGTCAGTCA 639 601 581 Antisense
Sequence Source Affymetrix Proprietary Database