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[181885_at]

Description F08B12.1 /REP_DB=WormBase Gene ID /WP=CE03162 /TR=Q19188 /GB=CAA92112.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 71      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 63      6grp_total > 80      4grp_total > 49      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 535
1 2 3 4 range
A 383.2
(-3)
P 		918.2
(3)
P 		770.7
(1.3)
P 		391.8
(-2.9)
P 		535
B 441.1
(-3)
P 		766.2
(3)
P 		699.5
(1.8)
P 		474.5
(-2.4)
P 		325
C 401.7
(-3)
P 		736.3
(2.6)
P 		757.2
(3)
P 		445.2
(-2.3)
P 		356
avr 408.7
(-3) 		806.9
(3) 		742.5
(2) 		437.2
(-2.6) 		398

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 351
1 2 3 4 5 6 range
A 437
(-3)
P 		663.9
(1.1)
P 		486.1
(-2.1)
P 		765.1
(3)
P 		556
(-0.8)
P 		569.9
(-0.6)
P 		328
B 449.4
(-3)
P 		610
(1.3)
P 		616
(1.5)
P 		671.5
(3)
P 		524
(-1)
P 		483.8
(-2.1)
P 		222
C 552.8
(-3)
P 		613.8
(-1.4)
P 		598.5
(-1.8)
P 		787.8
(3)
P 		731
(1.5)
P 		554.1
(-3)
P 		235
avr 479.7
(-3) 		629.2
(0.4) 		566.9
(-1) 		741.5
(3) 		603.7
(-0.2) 		535.9
(-1.7) 		262
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 139.5
(0.4)
P 		199.3
(2.9)
P 		201.5
(3)
P 		57.4
(-3)
P 		144
B 140.7
(-0.3)
P 		189.2
(3)
P 		162.1
(1.2)
P 		101.1
(-3)
P 		88
C 133
(-1.2)
P 		211.7
(1.3)
P 		263.9
(3)
P 		75.1
(-3)
P 		189
avr 137.7
(-0.3) 		200.1
(2.6) 		209.2
(3) 		77.9
(-3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 80.6
(-3)
P 		236.1
(3)
P 		105.9
(-2)
P 		194
(1.4)
P 		167.8
(0.4)
P 		138.8
(-0.8)
P 		156
B 156.4
(-2.4)
P 		203.1
(2.1)
P 		149.6
(-3)
P 		177.9
(-0.3)
P 		212.9
(3)
P 		180.4
(-0.1)
P 		63
C 204.9
(0.7)
P 		187.3
(-0.5)
P 		152.3
(-3)
P 		158.4
(-2.6)
P 		236.5
(3)
P 		185.5
(-0.6)
P 		84
avr 147.3
(-2.1) 		208.8
(3) 		135.9
(-3) 		176.8
(0.4) 		205.7
(2.7) 		168.2
(-0.3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181885_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17070
Sequence Type Exemplar sequence
Representative Public ID F08B12.1 NCBI
Target Description F08B12.1 /REP_DB=WormBase Gene ID /WP=CE03162 /TR=Q19188 /GB=CAA92112.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077506(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077506 NCBI Caenorhabditis elegans F08B12.1 (F08B12.1) mRNA, complete cds. 11/11 None
GENEFINDER00000001953 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11385039:11392410:1 11/11 None
SNAP00000001944 ENSEMBL cdna:SNAP chromosome:CEL140:X:11388901:11392331:1 9/11 None
F08B12.1 ENSEMBL cdna:known chromosome:CEL140:X:11385039:11392703:1 gene:F08B12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11385038-11392331(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08B12.1
Entrez Gene 181330 Entrez gene
SwissProt Q19188 EMBL-EBI
WormBase CE03162 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23488.1.S1_S_AT similar to Prominin 1 precursor (Prominin-like protein 1) (Antigen AC133) (CD133 antigen) cfa
DROSOPHILA_2:1641333_S_AT dm
DROSGENOME1:154220_AT dm
CHICKEN:GGAAFFX.11899.1.S1_AT prominin 1 gga
CHICKEN:GGAAFFX.9212.1.S1_S_AT prominin 1 gga
CHICKEN:GGAAFFX.9212.6.S1_S_AT prominin 1 gga
HG-FOCUS:204304_S_AT prominin 1 hs
HG-U133_PLUS_2:204304_S_AT prominin 1 hs
HG-U133A:204304_S_AT prominin 1 hs
HG-U133A_2:204304_S_AT prominin 1 hs
HU35KSUBB:RC_R40057_AT prominin 1 hs
U133_X3P:G5174386_3P_A_AT prominin 1 hs
HG-U95AV2:41470_AT prominin 1 hs
HG-U95D:71478_AT Prominin 1 hs
MG-U74AV2:93389_AT prominin 1 mm
MG-U74AV2:93390_G_AT prominin 1 mm
MOUSE430_2:1419700_A_AT prominin 1 mm
MOE430A:1419700_A_AT prominin 1 mm
MOUSE430A_2:1419700_A_AT prominin 1 mm
MU11KSUBA:AF026269_AT prominin 1 mm
RAE230A:1368008_AT prominin 1 rn
RAT230_2:1368008_AT prominin 1 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92112 Hypothetical protein F08B12.1 [Caenorhabditis elegans] ref|NP_509907.1| F08B12.1 [Caenorhabditis elegans] 0.0
blast CAE70647 Hypothetical protein CBG17350 [Caenorhabditis briggsae] 0.0
blast CAA92112 Hypothetical protein F08B12.1 [Caenorhabditis elegans] ref|NP_509907.1| F08B12.1 [Caenorhabditis elegans] 1.0E-159
blast CAE70647 Hypothetical protein CBG17350 [Caenorhabditis briggsae] 1.0E-150
blast AAS19709 prominin 1 isoform s7 [Homo sapiens] 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR008795 EMBL-EBI Prominin 1.0E-126
Pfam IPR008795 EMBL-EBI Prominin 1.0E-126
Pfam IPR008795 EMBL-EBI Prominin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509907.1 6 5-22,102-124,152-174,432-454,475-497,792-814
F08B12.1 6 5-22,102-124,152-174,432-454,475-497,792-814
GENEFINDER00000001953 6 5-22,102-124,152-174,432-454,475-497,792-814
SNAP00000001944 3 12-34,295-317,547-569
Sequence
Target Sequence 
>C. ELEGANS:181885_AT
gcaaattcgggtcgtcctaaggcagtgactcttgttcttgaacaaatcagagagttagag
attcaatttgcgaaaccattaaggagcagattagaggcattgtactctaacattacttca
ctcgatgaaaaactttctcagcttcaagttcctgtttcttctcttcttgtaaagcttcag
catgctcaggctttactggctgaaaacatgagagagcatttcggtcgtgcagctaaagaa
gaattccaacaaatggttaacaacatcgaccagtacatagttcatgtcaaaagccagatg
gagagcgaggtcacgtcatgtcaaccgttgacacaaattgcccggcaaacaactgctgct
gtttgtgcttacacaattgatccatataacgggacatggatgtgtatgctgatctgccta
gttctgttgataccaattgtgataatttcaaactcattaataggtttatactctaagatg
cacgcgttcccaaaatacattgtggaaccaccatctgaaacacatcacatgtcatcgttc
attaccgatacatatgacactaggcctaagcctggatacgcaaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAATTCGGGTCGTCCTAAGGCAG 161 317 1990 Antisense
AGGCAGTGACTCTTGTTCTTGAACA 283 55 2009 Antisense
CAGCTTCAAGTTCCTGTTTCTTCTC 121 205 2128 Antisense
AGCTTCAGCATGCTCAGGCTTTACT 429 79 2162 Antisense
GAGAGAGCATTTCGGTCGTGCAGCT 411 395 2199 Antisense
TGGAGAGCGAGGTCACGTCATGTCA 61 549 2288 Antisense
AACAACTGCTGCTGTTTGTGCTTAC 444 135 2337 Antisense
TGCTGATCTGCCTAGTTCTGTTGAT 606 581 2396 Antisense
TATACTCTAAGATGCACGCGTTCCC 56 661 2456 Antisense
GGAACCACCATCTGAAACACATCAC 433 529 2493 Antisense
AGGCCTAAGCCTGGATACGCAAACT 307 1 2551 Antisense
Sequence Source Affymetrix Proprietary Database