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[181874_at]

Description W03F8.6 /REP_DB=WormBase Gene ID /WP=CE17276 /TR=O44567 /GB=AAB94187.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 33      4grp_highest_8_8 > 04      4grp_high_8_8 > 38      6grp_highest_nonorm > 62      6grp_total > 11      4grp_total > 33      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 729
1 2 3 4 range
A 328.3
(-3)
P 		590.8
(1.5)
P 		539
(0.6)
P 		676.5
(3)
P 		348
B 263.1
(-3)
P 		991.8
(3)
P 		603.9
(-0.2)
P 		758
(1.1)
P 		729
C 297.6
(-3)
P 		816.8
(3)
P 		763.2
(2.4)
P 		660.9
(1.2)
P 		519
avr 296.3
(-3) 		799.8
(3) 		635.4
(1) 		698.5
(1.8) 		504

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 535
1 2 3 4 5 6 range
A 303.8
(-3)
P 		832.4
(3)
P 		559.4
(-0.1)
P 		508.9
(-0.7)
P 		377
(-2.2)
P 		326.6
(-2.7)
P 		529
B 517.1
(1.3)
P 		600.9
(3)
P 		484.2
(0.7)
P 		341.7
(-2.1)
P 		540.6
(1.8)
P 		297.5
(-3)
P 		303
C 520.9
(0.2)
P 		686.8
(3)
P 		492.5
(-0.3)
P 		424.1
(-1.4)
P 		477.9
(-0.5)
P 		330.9
(-3)
P 		356
avr 447.3
(-1) 		706.7
(3) 		512
(-0) 		424.9
(-1.4) 		465.2
(-0.7) 		318.3
(-3) 		388
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 119.5
(-0.1)
P 		128.3
(1.2)
P 		140.9
(3)
P 		99
(-3)
P 		42
B 83.9
(-3)
P 		245
(3)
P 		139.9
(-0.9)
P 		161.5
(-0.1)
P 		161
C 98.5
(-3)
P 		234.8
(1.9)
P 		266
(3)
P 		111.4
(-2.5)
P 		168
avr 100.6
(-3) 		202.7
(3) 		182.3
(1.8) 		124
(-1.6) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 240
1 2 3 4 5 6 range
A 56
(-3)
P 		296.1
(3)
P 		121.8
(-1.4)
P 		129.1
(-1.2)
P 		113.8
(-1.6)
P 		79.5
(-2.4)
P 		240
B 179.9
(1.2)
P 		200
(2.1)
P 		117.6
(-1.7)
P 		90.5
(-3)
P 		219.6
(3)
P 		110.9
(-2.1)
P 		129
C 193.1
(2.2)
P 		209.6
(3)
P 		125.4
(-1.1)
P 		85.3
(-3)
P 		154.7
(0.3)
P 		110.8
(-1.8)
P 		124
avr 143
(-1.1) 		235.2
(3) 		121.6
(-2.1) 		101.6
(-2.9) 		162.7
(-0.2) 		100.4
(-3) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181874_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10756
Sequence Type Exemplar sequence
Representative Public ID W03F8.6 NCBI
Target Description W03F8.6 /REP_DB=WormBase Gene ID /WP=CE17276 /TR=O44567 /GB=AAB94187.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068335(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068335 NCBI Caenorhabditis elegans W03F8.6 (W03F8.6) mRNA, complete cds. 11/11 None
SNAP00000036703 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5185756:5186552:-1 11/11 None
W03F8.6 ENSEMBL cdna:known chromosome:CEL140:IV:5185513:5186569:-1 gene:W03F8.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000036713 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5185761-5186558(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F8.6
Entrez Gene 189159 Entrez gene
SwissProt O44567 EMBL-EBI
WormBase CE17276 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94187 Hypothetical protein W03F8.6 [Caenorhabditis elegans] ref|NP_500736.1| W03F8.6 [Caenorhabditis elegans] 1.0E-61
blast CAE72828 Hypothetical protein CBG20110 [Caenorhabditis briggsae] 8.0E-52
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500736.1 2 5-27,109-126
W03F8.6 2 5-27,109-126
SNAP00000036703 2 5-27,109-126
Sequence
Target Sequence 
>C. ELEGANS:181874_AT
atgactcaaatctcagctattcgtttggcggttttctttgttcttgtaccaactacattg
gcagcaatccaatgttgggttggacaggagactgtttcaaacggaagaagactgccaaca
gaaatcggaggcccttatcaatctgtacaatgcaacgatgccgacttctgcttcaatagt
tatgtaagacgacacaagaaaggagatgatagttatacaatcaccaagagttgcggagaa
gttggaaaatgcttcgaagacggttgcattggcccaggagacgagaagaattgctgctgc
agtgacaatatgtgcaactcgagcaacggtctgatctatccaacagccggaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACTCAAATCTCAGCTATTCGTT 225 45 13 Antisense
GCTATTCGTTTGGCGGTTTTCTTTG 186 303 28 Antisense
TTTCTTTGTTCTTGTACCAACTACA 696 671 45 Antisense
CCAACAGAAATCGGAGGCCCTTATC 168 275 127 Antisense
GCCCTTATCAATCTGTACAATGCAA 700 285 143 Antisense
AATGCAACGATGCCGACTTCTGCTT 661 165 161 Antisense
CCGACTTCTGCTTCAATAGTTATGT 701 261 173 Antisense
CGGTTGCATTGGCCCAGGAGACGAG 431 243 273 Antisense
GAGACGAGAAGAATTGCTGCTGCAG 102 395 290 Antisense
GCAACTCGAGCAACGGTCTGATCTA 43 321 326 Antisense
GTCTGATCTATCCAACAGCCGGAAT 4 473 341 Antisense
Sequence Source Affymetrix Proprietary Database