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[181873_at]

Description K04B12.2 /REP_DB=WormBase Gene ID /WP=CE16225 /TR=O45656 /GB=CAB05754.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 50      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 52      6grp_total > 40      4grp_total > 47      6grp_highest_sub > 04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 554.6
(3)
P 		538.6
(2.4)
P 		381.2
(-3)
P 		423.8
(-1.5)
P 		173
B 596.6
(3)
P 		354
(-2.8)
P 		378.8
(-2.2)
P 		347.6
(-3)
P 		249
C 607.8
(3)
P 		382.5
(-3)
P 		413
(-2.2)
P 		431.2
(-1.7)
P 		225
avr 586.3
(3) 		425
(-2) 		391
(-3) 		400.9
(-2.7) 		195

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 546.8
(-0.9)
P 		752.5
(3)
P 		601
(0.1)
P 		681.7
(1.7)
P 		434.1
(-3)
P 		499.8
(-1.8)
P 		318
B 553.7
(0.3)
P 		624.9
(2.1)
P 		658.6
(3)
P 		644.1
(2.6)
P 		422.3
(-3)
P 		478.3
(-1.6)
P 		236
C 493.4
(-2.6)
P 		585.3
(-0.7)
P 		723.7
(2.1)
P 		765.5
(3)
P 		473
(-3)
P 		502
(-2.4)
P 		293
avr 531.3
(-0.9) 		654.2
(2) 		661.1
(2.1) 		697.1
(3) 		443.1
(-3) 		493.4
(-1.8) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 201.9
(3)
P 		116.9
(-0.6)
P 		99.6
(-1.4)
P 		62
(-3)
P 		140
B 190.3
(3)
P 		87.4
(-2.3)
P 		87.8
(-2.3)
P 		74.1
(-3)
P 		116
C 201.2
(3)
P 		110
(-1.3)
P 		143.9
(0.3)
P 		72.7
(-3)
P 		129
avr 197.8
(3) 		104.8
(-1.4) 		110.4
(-1.1) 		69.6
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 100.8
(-3)
P 		267.6
(3)
P 		130.9
(-1.9)
P 		172.9
(-0.4)
P 		131
(-1.9)
P 		121.7
(-2.2)
P 		167
B 192.7
(1.1)
P 		208
(3)
P 		159.9
(-3)
P 		170.6
(-1.7)
P 		171.6
(-1.5)
P 		178.3
(-0.7)
P 		48
C 182.9
(2.8)
P 		178.6
(1.9)
P 		184.2
(3)
P 		154
(-2.8)
P 		153
(-3)
P 		168
(-0.1)
P 		31
avr 158.8
(-2.4) 		218.1
(3) 		158.3
(-2.4) 		165.8
(-1.7) 		151.9
(-3) 		156
(-2.6) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181873_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3312
Sequence Type Exemplar sequence
Representative Public ID K04B12.2 NCBI
Target Description K04B12.2 /REP_DB=WormBase Gene ID /WP=CE16225 /TR=O45656 /GB=CAB05754.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027061(11), NM_001027060(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027061 NCBI Caenorhabditis elegans K04B12.2b (K04B12.2) mRNA, complete cds. 11/11 None
NM_001027060 NCBI Caenorhabditis elegans K04B12.2a (K04B12.2) mRNA, complete cds. 11/11 None
SNAP00000010721 ENSEMBL cdna:SNAP chromosome:CEL140:II:14434911:14436616:-1 11/11 None
GENEFINDER00000010724 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14434911:14437854:-1 11/11 None
K04B12.2b.2 ENSEMBL cdna:known chromosome:CEL140:II:14434342:14437862:-1 gene:K04B12.2 11/11 A 
K04B12.2b.3 ENSEMBL cdna:known chromosome:CEL140:II:14434406:14437870:-1 gene:K04B12.2 11/11 None
K04B12.2b.1 ENSEMBL cdna:known chromosome:CEL140:II:14434434:14438867:-1 gene:K04B12.2 11/11 None
K04B12.2b.4 ENSEMBL cdna:known chromosome:CEL140:II:14434477:14436616:-1 gene:K04B12.2 11/11 None
K04B12.2a ENSEMBL cdna:known chromosome:CEL140:II:14434911:14437854:-1 gene:K04B12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14434907-14438089(-) 97.19 100.0
Public Domain and Genome References
Ensembl K04B12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05754 Hypothetical protein K04B12.2a [Caenorhabditis elegans] ref|NP_001022231.1| K04B12.2a [Caenorhabditis elegans] 0.0
blast CAE46672 Hypothetical protein K04B12.2b [Caenorhabditis elegans] ref|NP_001022232.1| K04B12.2b [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:181873_AT
aaatatcgtgtcacaaccgtccgaagggaaaaaatctgctagacgaggagtttttctgtc
ttgctccgtgctgcagcggaatgaggctattccaagccgagacgtacacttttctcccag
gatttgtcaaaaaccgtctgaagcaagctgatgattgccgagtaatagtgcataaaacct
caaatggaattgaattgatcacaccaataaagtggcagcgagtcacgccaatctttcctg
agaatacaaaaaacatccagcctccagatgagagaatagaagaagacgaagctgaagaag
aggatccattggcaaatcgggaaattgtagagcaacttatgcacgaatacttcattccga
cacctgcattgcctctagacgacttggttttcggtccctatcttcaacgagttcgagaat
cagagccatttccggagaacctgctccacgatctagttcggcgggtcaattcaaacttgg
acaatagctacgatagtcatcaagagctggagttgttcgacaaattggacgaagtcccca
ttgacgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATATCGTGTCACAACCGTCCGAA 518 115 848 Antisense
GAGGAGTTTTTCTGTCTTGCTCCGT 77 403 892 Antisense
CTTGCTCCGTGCTGCAGCGGAATGA 249 219 907 Antisense
GCTATTCCAAGCCGAGACGTACACT 136 305 933 Antisense
GTACACTTTTCTCCCAGGATTTGTC 104 461 951 Antisense
AGTGGCAGCGAGTCACGCCAATCTT 451 59 1058 Antisense
CACGAATACTTCATTCCGACACCTG 279 199 1189 Antisense
ATTGCCTCTAGACGACTTGGTTTTC 207 7 1215 Antisense
GTCCCTATCTTCAACGAGTTCGAGA 552 475 1241 Antisense
TCCACGATCTAGTTCGGCGGGTCAA 167 589 1292 Antisense
TTGGACGAAGTCCCCATTGACGAGA 668 665 1372 Antisense
Sequence Source Affymetrix Proprietary Database