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[181867_s_at]

Description W03F8.4 /REP_DB=WormBase Gene ID /WP=CE17274 /TR=O44566 /GB=AAB94192.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 86      6grp_total > 89      4grp_total > 61      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 745
1 2 3 4 range
A 989.8
(3)
P 		730.8
(0.9)
P 		851
(1.9)
P 		244.6
(-3)
P 		745
B 978.2
(3)
P 		800.1
(1.4)
P 		793.8
(1.4)
P 		306.7
(-3)
P 		672
C 964.8
(3)
P 		903.5
(2.5)
P 		872.5
(2.2)
P 		279.3
(-3)
P 		686
avr 977.6
(3) 		811.5
(1.6) 		839.1
(1.8) 		276.9
(-3) 		701

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 585
1 2 3 4 5 6 range
A 1045.5
(1.6)
P 		867.8
(-0.7)
P 		852
(-0.9)
P 		684.5
(-3)
P 		1153.2
(3)
P 		950
(0.4)
P 		469
B 996
(-1.2)
P 		882.7
(-3)
P 		985.6
(-1.4)
P 		913.7
(-2.5)
P 		1152.3
(1.2)
P 		1269.9
(3)
P 		387
C 1017.4
(0.5)
P 		854
(-1.4)
P 		707.1
(-3)
P 		959.3
(-0.2)
P 		956.5
(-0.2)
P 		1245.2
(3)
P 		538
avr 1019.6
(0.4) 		868.2
(-2.6) 		848.2
(-3) 		852.5
(-2.9) 		1087.3
(1.7) 		1155
(3) 		307
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 325
1 2 3 4 range
A 360.4
(3)
P 		158.6
(-0.7)
P 		222.5
(0.5)
P 		35.8
(-3)
P 		325
B 311.9
(3)
P 		197.6
(0.2)
P 		183.9
(-0.1)
P 		65.3
(-3)
P 		247
C 319.4
(3)
P 		259.8
(1.7)
P 		304.1
(2.7)
P 		47.1
(-3)
P 		272
avr 330.6
(3) 		205.3
(0.3) 		236.8
(1) 		49.4
(-3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 300
1 2 3 4 5 6 range
A 192.7
(-2.3)
P 		308.6
(1.6)
P 		185.6
(-2.6)
P 		173.6
(-3)
P 		348
(3)
P 		231.3
(-1)
P 		174
B 346.6
(-0.3)
P 		293.8
(-1.6)
P 		239.3
(-3)
P 		242
(-2.9)
P 		468.2
(2.9)
P 		473.5
(3)
P 		234
C 377.1
(2)
P 		260.6
(-1)
P 		180
(-3)
P 		192.9
(-2.7)
P 		309.5
(0.3)
P 		416.8
(3)
P 		237
avr 305.5
(0.6) 		287.7
(-0) 		201.6
(-3) 		202.8
(-3) 		375.2
(3) 		373.9
(3) 		174
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181867_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10584
Sequence Type Exemplar sequence
Representative Public ID W03F8.4 NCBI
Target Description W03F8.4 /REP_DB=WormBase Gene ID /WP=CE17274 /TR=O44566 /GB=AAB94192.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068334(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068334 NCBI Caenorhabditis elegans W03F8.4 (W03F8.4) mRNA, complete cds. 11/11 None
SNAP00000036702 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5184113:5184877:1 10/11 None
GENEFINDER00000036712 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5184113:5184877:1 11/11 None
W03F8.4 ENSEMBL cdna:known chromosome:CEL140:IV:5184113:5185222:1 gene:W03F8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5184118-5184883(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F8.4
Entrez Gene 177293 Entrez gene
SwissProt O44566 EMBL-EBI
WormBase CE17274 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1589788_AT similar to PRPK (p53-related protein kinase)-binding protein cfa
CANINE_2:CFA.15278.1.A1_AT similar to PRPK (p53-related protein kinase)-binding protein cfa
CANINE_2:CFA.15278.1.A1_S_AT similar to PRPK (p53-related protein kinase)-binding protein cfa
CANINE_2:CFAAFFX.13922.1.S1_S_AT similar to PRPK (p53-related protein kinase)-binding protein cfa
CANINE_2:CFAAFFX.13925.1.S1_AT similar to PRPK (p53-related protein kinase)-binding protein cfa
CHICKEN:GGA.11998.1.S1_AT similar to CGI-121 protein gga
HG-U133_PLUS_2:219030_AT CGI-121 protein hs
HG-U133A:219030_AT CGI-121 protein hs
HG-U133A_2:219030_AT CGI-121 protein hs
HU35KSUBA:C02352_S_AT CGI-121 protein hs
HU35KSUBD:RC_W02072_R_AT CGI-121 protein hs
U133_X3P:G7705589_3P_AT CGI-121 protein hs
HG-U95C:61373_AT CGI-121 protein hs
HG-U95C:65589_AT CGI-121 protein hs
MOUSE430_2:1425410_AT RIKEN cDNA 1810034M08 gene mm
MOUSE430A_2:1425410_AT RIKEN cDNA 1810034M08 gene mm
MOE430A:1425410_AT RIKEN cDNA 1810034M08 gene mm
MOUSE430A_2:1425682_A_AT RIKEN cDNA 1810034M08 gene mm
MOE430A:1425682_A_AT RIKEN cDNA 1810034M08 gene mm
MOUSE430_2:1425682_A_AT RIKEN cDNA 1810034M08 gene mm
MG-U74BV2:114672_AT RIKEN cDNA 1810034M08 gene mm
MOE430A:1424992_AT RIKEN cDNA 1810034M08 gene mm
MOUSE430A_2:1424992_AT RIKEN cDNA 1810034M08 gene mm
MOUSE430_2:1424992_AT RIKEN cDNA 1810034M08 gene mm
MG-U74BV2:111921_AT RIKEN cDNA 1810034M08 gene, mRNA (cDNA clone MGC:38694 IMAGE:5357332) mm
MU19KSUBB:TC30470_AT RIKEN cDNA 1810034M08 gene, mRNA (cDNA clone MGC:38694 IMAGE:5357332) mm
MU19KSUBB:TC30503_AT RIKEN cDNA 1810034M08 gene, mRNA (cDNA clone MGC:38694 IMAGE:5357332) mm
MU19KSUBB:TC34577_AT RIKEN cDNA 1810034M08 gene, mRNA (cDNA clone MGC:38694 IMAGE:5357332) mm
RG-U34C:RC_AA819730_AT similar to CGI-121 protein (predicted) rn
RAE230B:1390972_AT similar to CGI-121 protein (predicted) rn
RAT230_2:1390972_AT similar to CGI-121 protein (predicted) rn
RAE230B:1382014_AT similar to CGI-121 protein (predicted) rn
RAT230_2:1382014_AT similar to CGI-121 protein (predicted) rn
RG-U34B:RC_AI013886_AT Similar to CGI-121 protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAB94192 Hypothetical protein W03F8.4 [Caenorhabditis elegans] ref|NP_500735.1| W03F8.4 [Caenorhabditis elegans] 2.0E-98
blast CAE72829 Hypothetical protein CBG20111 [Caenorhabditis briggsae] 4.0E-91
blast AAB94192 Hypothetical protein W03F8.4 [Caenorhabditis elegans] ref|NP_500735.1| W03F8.4 [Caenorhabditis elegans] 7.0E-90
blast CAE72829 Hypothetical protein CBG20111 [Caenorhabditis briggsae] 1.0E-82
blast NP_789812 Prpk (p53-related protein kinase)-binding protein [Mus musculus] gb|AAH24858.1| CGI-121 protein [Mus musculus] gb|AAH27162.1| 1810034M08Rik protein [Mus musculus] 3.0E-25
Sequence
Target Sequence 
>C. ELEGANS:181867_S_AT
ttatgagcttcaaccagatccgtacgattttacacaacggaagacatgcagagtttgtct
attcaaggatgttaaaaatgcggcagaactctcgcaacagttgaaggaaggaaaaatcga
tgcagctttaattcgtgcggaacttgtgctcgaaccgttcgtactcctcgccgccgccaa
ccgggccgttcatcaatcggctcataatcggatgagttgtcggagtctagctgcggagct
tgtctattcgctttctccgagtagaaatattaccgattctctagtgaccttcggaattgc
cgagcactcgactgcaattatcgccgcaattttcgacgatgattccggaaaagcaatgaa
aaagttggcgaaagcaatcaagggaacacctgtgccattaatggaaggcctaccgaaatt
cgcaaatgttaacatgatcaagaaggtttatcaagtaggaaatccagcatttgtcgaaga
aggactctccgatcacatcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATGAGCTTCAACCAGATCCGTAC 21 681 30 Antisense
AATGCGGCAGAACTCTCGCAACAGT 135 167 106 Antisense
CGTGCGGAACTTGTGCTCGAACCGT 624 243 163 Antisense
CAACCGGGCCGTTCATCAATCGGCT 219 187 207 Antisense
TGAGTTGTCGGAGTCTAGCTGCGGA 472 565 242 Antisense
AGCTGCGGAGCTTGTCTATTCGCTT 279 81 258 Antisense
TCTCTAGTGACCTTCGGAATTGCCG 126 607 307 Antisense
GGAATTGCCGAGCACTCGACTGCAA 630 533 322 Antisense
GCAATTATCGCCGCAATTTTCGACG 287 323 343 Antisense
ATGGAAGGCCTACCGAAATTCGCAA 630 51 430 Antisense
AAGAAGGACTCTCCGATCACATCGT 397 151 506 Antisense
Sequence Source Affymetrix Proprietary Database