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[181865_s_at]

Description C10E2.3 /REP_DB=WormBase Gene ID /WP=CE08062 /TR=O17323 /GB=AAB71243.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 19      6grp_cgc > 01      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 73.2
(-1.3)
M 		87.7
(0.4)
A 		58.6
(-3)
A 		109.3
(3)
P 		51
B 63.9
(-3)
P 		77.1
(-1.8)
A 		70.5
(-2.4)
P 		131.9
(3)
P 		68
C 64.6
(-0.5)
P 		74.4
(0.2)
A 		27.5
(-3)
A 		115.4
(3)
A 		88
avr 67.2
(-1.7) 		79.7
(-0.5) 		52.2
(-3) 		118.9
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 80.9
(3)
A 		47.5
(0.3)
A 		50.6
(0.5)
A 		27.3
(-1.4)
A 		45.8
(0.1)
A 		7.7
(-3)
A 		73
B 89
(3)
P 		34.4
(-1.7)
A 		19.3
(-3)
A 		61.7
(0.6)
M 		49.6
(-0.4)
A 		50.9
(-0.3)
A 		70
C 63.9
(3)
P 		24.3
(-3)
A 		30.3
(-2.1)
A 		26.1
(-2.7)
A 		47.2
(0.5)
P 		52.1
(1.2)
A 		40
avr 77.9
(3) 		35.4
(-2.7) 		33.4
(-3) 		38.4
(-2.3) 		47.5
(-1.1) 		36.9
(-2.5) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 26.7
(3)
M 		19
(-1.1)
A 		15.3
(-3)
A 		16
(-2.6)
P 		11
B 20.4
(-0.9)
P 		19.1
(-1.6)
A 		16.3
(-3)
P 		28.1
(3)
P 		12
C 21.4
(3)
P 		21.4
(3)
A 		9.6
(-3)
A 		19.5
(2)
A 		12
avr 22.8
(3) 		19.8
(1) 		13.7
(-3) 		21.2
(1.9) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 14.9
(2.2)
A 		16.9
(3)
A 		11
(0.6)
A 		6.9
(-1)
A 		13.8
(1.8)
A 		1.9
(-3)
A 		15
B 31
(3)
P 		11.5
(-1.4)
A 		4.7
(-3)
A 		16.4
(-0.3)
M 		20.2
(0.5)
A 		19
(0.3)
A 		26
C 23.7
(3)
P 		7.4
(-2.3)
A 		7.7
(-2.2)
A 		5.2
(-3)
A 		15.3
(0.3)
P 		17.4
(1)
A 		19
avr 23.2
(3) 		11.9
(-1.4) 		7.8
(-3) 		9.5
(-2.3) 		16.4
(0.4) 		12.8
(-1.1) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181865_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17626
Sequence Type Exemplar sequence
Representative Public ID C10E2.3 NCBI
Target Description C10E2.3 /REP_DB=WormBase Gene ID /WP=CE08062 /TR=O17323 /GB=AAB71243.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078299(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078299 NCBI Caenorhabditis elegans Histone DeAcetylase family member (hda-4) (hda-4) mRNA, complete cds. 9/11 None
SNAP00000026943 ENSEMBL cdna:SNAP chromosome:CEL140:X:16740360:16743672:1 9/11 None
GENEFINDER00000026954 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16740360:16744548:1 9/11 None
C10E2.3 ENSEMBL cdna:known chromosome:CEL140:X:16739748:16744611:1 gene:C10E2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16740359-16744333(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hda-4
Ensembl C10E2.3
Entrez Gene 181723 Entrez gene
SwissProt O17323 EMBL-EBI
WormBase CE37192 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257729_AT histone deacetylase family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16575 histone deacetylation inferred from sequence similarity QuickGO AmiGO
16568 chromatin modification inferred from sequence similarity QuickGO AmiGO
6915 apoptosis inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from sequence similarity QuickGO AmiGO
5737 cytoplasm inferred from sequence similarity QuickGO AmiGO
118 histone deacetylase complex inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4407 histone deacetylase activity inferred from sequence similarity QuickGO AmiGO
8134 transcription factor binding inferred from physical interaction QuickGO AmiGO
3714 transcription corepressor activity inferred from sequence similarity QuickGO AmiGO
5516 calmodulin binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_510700 Histone DeAcetylase family member (hda-4) [Caenorhabditis elegans] sp|O17323|HDA4_CAEEL Histone deacetylase 4 (Histone deacetylase 7) (CeHDA-7) 0.0
blast AAZ82858 Histone deacetylase protein 4 [Caenorhabditis elegans] 0.0
blast Q613L4 Histone deacetylase 4 emb|CAE69945.1| Hypothetical protein CBG16328 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000286 EMBL-EBI Histone deacetylase superfamily 1.0E-126
Pfam IPR000286 EMBL-EBI Histone deacetylase superfamily 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:181865_S_AT
taaatgtgccattctccggagatgtgatgcgagacccagagtacttggccgcgtggagaa
ccgttattgagccagtaatggctagtttttgcccagattttatcattgtgtcagccggat
ttgatgcttgccacggacacccgaacgcacttggcggatatgaagtcacaccggaaatgt
ttggatatatgacaaaatcgttgttgaattatgcgagtgggaaagttgtgctggctttag
aagggggctacgatttgaaaagcatttctgaagccgctcaacagtgtgttcaggcactca
ttggcgaatccgatgacgcaggtcggttgagctcagttgctctcgaatcattacctaatc
caagtgcagttgagacgttacaaaagtctcgtcaatttaatcagcattttatgtcttctc
cctctgtctccccagtgatatatttttttcttccactccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAATGTGCCATTCTCCGGAGATGT 362 639 1892 Antisense
TGCGAGACCCAGAGTACTTGGCCGC 104 555 1919 Antisense
TACTTGGCCGCGTGGAGAACCGTTA 633 103 1933 Antisense
GTCAGCCGGATTTGATGCTTGCCAC 637 465 2001 Antisense
ACACCCGAACGCACTTGGCGGATAT 102 87 2028 Antisense
GCATTTCTGAAGCCGCTCAACAGTG 54 309 2153 Antisense
GTGTTCAGGCACTCATTGGCGAATC 549 485 2177 Antisense
CGCAGGTCGGTTGAGCTCAGTTGCT 60 239 2208 Antisense
TCAGTTGCTCTCGAATCATTACCTA 599 623 2224 Antisense
AATCAGCATTTTATGTCTTCTCCCT 442 171 2290 Antisense
TGATATATTTTTTTCTTCCACTCCC 306 565 2327 Antisense
Sequence Source Affymetrix Proprietary Database