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[181861_at]

Description F13A2.4 /REP_DB=WormBase Gene ID /WP=CE09366 /TR=O16893 /GB=AAB69897.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 59      4grp_total > 35      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12.3
(-3)
A 		16.7
(0.7)
A 		13.3
(-2.2)
A 		19.5
(3)
A 		7
B 12.4
(2.2)
A 		13
(3)
A 		9.8
(-1.4)
A 		8.6
(-3)
A 		4
C 7.8
(-2.7)
A 		16.5
(3)
A 		7.3
(-3)
A 		9.6
(-1.5)
A 		9
avr 10.8
(-2.2) 		15.4
(3) 		10.1
(-3) 		12.6
(-0.2) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 66.1
(3)
A 		11.8
(-2.3)
A 		6.9
(-2.8)
A 		5.1
(-3)
A 		16.5
(-1.9)
A 		43.4
(0.8)
A 		61
B 8.4
(-2.5)
A 		10.9
(-2)
A 		6.8
(-2.9)
A 		35
(3)
A 		6.2
(-3)
A 		19
(-0.3)
A 		29
C 8.6
(-2.2)
A 		15.4
(-1.1)
A 		9.6
(-2.1)
A 		5.5
(-2.7)
A 		3.6
(-3)
A 		41.7
(3)
A 		38
avr 27.7
(1.4) 		12.7
(-1.9) 		7.8
(-3) 		15.2
(-1.3) 		8.8
(-2.8) 		34.7
(3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 4.5
(3)
A 		3.6
(-0.4)
A 		3.5
(-0.8)
A 		2.9
(-3)
A 		2
B 4
(3)
A 		3.2
(0.8)
A 		2.3
(-1.6)
A 		1.8
(-3)
A 		2
C 2.6
(-1.1)
A 		4.8
(3)
A 		2.5
(-1.3)
A 		1.6
(-3)
A 		3
avr 3.7
(2.3) 		3.9
(3) 		2.8
(-0.7) 		2.1
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 12.2
(3)
A 		4.2
(-1.4)
A 		1.5
(-2.9)
A 		1.3
(-3)
A 		5
(-1)
A 		10.6
(2.1)
A 		11
B 2.9
(-2.1)
A 		3.6
(-1.5)
A 		1.7
(-3)
A 		9.3
(3)
A 		2.5
(-2.4)
A 		7.1
(1.3)
A 		8
C 3.2
(-2)
A 		4.7
(-1.3)
A 		2.4
(-2.4)
A 		1.1
(-3)
A 		1.2
(-3)
A 		13.9
(3)
A 		13
avr 6.1
(-0.1) 		4.2
(-1.4) 		1.9
(-3) 		3.9
(-1.6) 		2.9
(-2.3) 		10.5
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181861_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15238
Sequence Type Exemplar sequence
Representative Public ID F13A2.4 NCBI
Target Description F13A2.4 /REP_DB=WormBase Gene ID /WP=CE09366 /TR=O16893 /GB=AAB69897.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071809(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071809 NCBI Caenorhabditis elegans F13A2.4 (F13A2.4) mRNA, complete cds. 11/11 None
GENEFINDER00000037909 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4397164:4399317:-1 11/11 None
F13A2.4 ENSEMBL cdna:known chromosome:CEL140:V:4398533:4399317:-1 gene:F13A2.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037899 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4398545-4399317(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F13A2.4
Entrez Gene 184395 Entrez gene
SwissProt O16893 EMBL-EBI
WormBase CE09366 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB69897 Hypothetical protein F13A2.4 [Caenorhabditis elegans] ref|NP_504210.1| F13A2.4 [Caenorhabditis elegans] 3.0E-58
blast AAB69897 Hypothetical protein F13A2.4 [Caenorhabditis elegans] ref|NP_504210.1| F13A2.4 [Caenorhabditis elegans] 4.0E-55
blast CAE56048 Hypothetical protein CBG23614 [Caenorhabditis briggsae] 3.0E-20
blast CAE56048 Hypothetical protein CBG23614 [Caenorhabditis briggsae] 1.0E-19
Sequence
Target Sequence 
>C. ELEGANS:181861_AT
tgctgcccaatttggcattgtccccaacctcgcccatcaaagcgccgaggaacgcgctcg
tgacgcaccccggctcccattcctcatgaagctcaaccttctcgttgaagctcgaggaat
cacctcctttgaggatcttctgcaagcgaaaaccgaagttgagaaagagctggaggttgg
aaagcaaaaggatctcgctgaagacaaggagaacgatgttgaaagcgaagagcgactccc
ggaggaacccaaggcaaagaaggcgaaaaaatctccgaagatcaagggcttcccaaacct
agtgagaagaaatgcccgccgcggcatgggtgtccttggaggtgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTGCCCAATTTGGCATTGTCCCC 15 583 27 Antisense
CTCGCCCATCAAAGCGCCGAGGAAC 702 233 55 Antisense
AAAGCGCCGAGGAACGCGCTCGTGA 96 125 65 Antisense
CCATTCCTCATGAAGCTCAACCTTC 336 269 103 Antisense
AGCTCAACCTTCTCGTTGAAGCTCG 300 49 116 Antisense
GAAGCTCGAGGAATCACCTCCTTTG 88 343 133 Antisense
CCTCCTTTGAGGATCTTCTGCAAGC 354 267 149 Antisense
TGAAAGCGAAGAGCGACTCCCGGAG 394 569 246 Antisense
GATCAAGGGCTTCCCAAACCTAGTG 338 421 306 Antisense
CCTAGTGAGAAGAAATGCCCGCCGC 16 269 324 Antisense
CGGCATGGGTGTCCTTGGAGGTGAG 173 241 348 Antisense
Sequence Source Affymetrix Proprietary Database