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[181855_at]

Description F58A6.1 /REP_DB=WormBase Gene ID /WP=CE04675 /TR=Q20959 /GB=AAA96199.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 46      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 67      6grp_total > 47      4grp_total > 08      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2957
1 2 3 4 range
A 1362.1
(-3)
P 		1767.8
(-2.2)
P 		2259.9
(-1.2)
P 		4319.5
(3)
P 		2957
B 1521.6
(-3)
P 		1656
(-2.7)
P 		2440.9
(-0.6)
P 		3845.9
(3)
P 		2324
C 1468.7
(-3)
P 		1583.7
(-2.7)
P 		2383.7
(-1)
P 		4183.4
(3)
P 		2715
avr 1450.8
(-3) 		1669.2
(-2.5) 		2361.5
(-1) 		4116.3
(3) 		2666

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1896
1 2 3 4 5 6 range
A 2761.4
(3)
P 		1397.8
(-1.4)
P 		2349.4
(1.7)
P 		1718.5
(-0.3)
P 		882.3
(-3)
P 		1893.8
(0.2)
P 		1879
B 2234.5
(2.6)
P 		1649.3
(-0.2)
P 		1819.2
(0.6)
P 		1477.2
(-1)
P 		1058.8
(-3)
P 		2321.2
(3)
P 		1262
C 2127.4
(1.4)
P 		1599.9
(-0.4)
P 		1703.9
(-0.1)
P 		1675.6
(-0.2)
P 		865.7
(-3)
P 		2592.4
(3)
P 		1727
avr 2374.4
(3) 		1549
(-0.4) 		1957.5
(1.3) 		1623.8
(-0.1) 		935.6
(-3) 		2269.1
(2.6) 		1439
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 447
1 2 3 4 range
A 495.9
(-0.3)
P 		383.7
(-3)
P 		590.8
(2)
P 		632.3
(3)
P 		249
B 485.2
(-1.9)
P 		409
(-3)
P 		565.6
(-0.7)
P 		819.6
(3)
P 		411
C 486.2
(-2.5)
P 		455.3
(-3)
P 		830.8
(3)
P 		705.4
(1)
P 		376
avr 489.1
(-1.6) 		416
(-3) 		662.4
(1.9) 		719.1
(3) 		303

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 602
1 2 3 4 5 6 range
A 509
(2.9)
P 		497.1
(2.6)
P 		511.7
(3)
P 		435.8
(1.1)
P 		266.2
(-3)
P 		461.1
(1.8)
P 		246
B 777.5
(1.9)
P 		549
(-1)
P 		441.8
(-2.4)
P 		391.3
(-3)
P 		430.2
(-2.5)
P 		865.6
(3)
P 		474
C 788.5
(2.2)
P 		488.2
(-0.9)
P 		433.7
(-1.4)
P 		337
(-2.4)
P 		280.1
(-3)
P 		867.8
(3)
P 		588
avr 691.7
(2.4) 		511.4
(-0.3) 		462.4
(-1) 		388
(-2.1) 		325.5
(-3) 		731.5
(3) 		406
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181855_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5065
Sequence Type Exemplar sequence
Representative Public ID F58A6.1 NCBI
Target Description F58A6.1 /REP_DB=WormBase Gene ID /WP=CE04675 /TR=Q20959 /GB=AAA96199.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062553(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062553 NCBI Caenorhabditis elegans F58A6.1 (F58A6.1) mRNA, complete cds. 11/11 None
SNAP00000019946 ENSEMBL cdna:SNAP chromosome:CEL140:II:5142910:5143932:1 11/11 None
GENEFINDER00000019956 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5142910:5143932:1 11/11 None
F58A6.1 ENSEMBL cdna:known chromosome:CEL140:II:5142910:5143932:1 gene:F58A6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5142907-5143930(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58A6.1
Entrez Gene 186486 Entrez gene
SwissProt Q20959 EMBL-EBI
WormBase CE04675 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA96199 Hypothetical protein F58A6.1 [Caenorhabditis elegans] ref|NP_494954.1| F58A6.1 [Caenorhabditis elegans] 1.0E-156
blast CAE59051 Hypothetical protein CBG02334 [Caenorhabditis briggsae] 1.0E-138
blast NP_494448 Y25C1A.13 [Caenorhabditis elegans] gb|AAD12843.1| Hypothetical protein Y25C1A.13 [Caenorhabditis elegans] 2.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 2.5E-30
Sequence
Target Sequence 
>C. ELEGANS:181855_AT
aaaccagttattctcgcaatgcatggttattgtcttggtgcagcattggatattgcaact
gcttgtgatgttcgtgttgctacaaaagacgctgtgctctctgtaaaggaagtagatatt
ggaatggctgcagatgtcggaacactcaatcgtttgcctaaaattgttggaaatcacagt
tggatcaaggatatttccctttccgctcgtcatttctcagctggagaagcacttcaattc
ggtcttcttagtcgcgtctacgatacccgtgaggaaatgataaatgaggtgttaaaaatg
gcgaaactgattgctttgaaaagtccggttggagtacagggaactaagaatgctttgaat
tacgcaagagatcatactgtggagaatagcttgaattatgtggccacttggaatatgtcc
ca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACCAGTTATTCTCGCAATGCATG 638 125 349 Antisense
GGTTATTGTCTTGGTGCAGCATTGG 119 507 373 Antisense
CTGCTTGTGATGTTCGTGTTGCTAC 15 239 407 Antisense
GACGCTGTGCTCTCTGTAAAGGAAG 363 381 436 Antisense
GGAATGGCTGCAGATGTCGGAACAC 567 531 469 Antisense
GTCGGAACACTCAATCGTTTGCCTA 506 471 484 Antisense
ATCAAGGATATTTCCCTTTCCGCTC 421 25 532 Antisense
CGCTCGTCATTTCTCAGCTGGAGAA 671 255 552 Antisense
TCAATTCGGTCTTCTTAGTCGCGTC 678 631 582 Antisense
TCGCGTCTACGATACCCGTGAGGAA 286 597 600 Antisense
ATGTGGCCACTTGGAATATGTCCCA 140 51 746 Antisense
Sequence Source Affymetrix Proprietary Database