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[181853_at]

Description B0281.4 /REP_DB=WormBase Gene ID /WP=CE07715 /TR=O16611 /GB=AAB66083.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 06      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 2
(-3)
A 		5.7
(-1.1)
A 		13.6
(3)
A 		3.2
(-2.4)
A 		12
B 18.6
(3)
A 		2.9
(-2.4)
A 		3.7
(-2.2)
A 		1.3
(-3)
A 		17
C 7.5
(-1.8)
A 		4.9
(-2.4)
A 		2.1
(-3)
A 		29.6
(3)
A 		28
avr 9.4
(1.3) 		4.5
(-3) 		6.5
(-1.3) 		11.4
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 2.6
(-2.5)
A 		1
(-3)
A 		0.9
(-3)
A 		4.1
(-2)
A 		20.3
(3)
A 		2.4
(-2.5)
A 		19
B 1.7
(-2.7)
A 		1.5
(-2.8)
A 		1.3
(-3)
A 		4.3
(-0.4)
A 		8.2
(3)
A 		6.9
(1.9)
A 		7
C 1.3
(-3)
A 		12.1
(0)
A 		2.3
(-2.7)
A 		2.7
(-2.6)
A 		7.9
(-1.1)
A 		22.6
(3)
A 		21
avr 1.9
(-2.8) 		4.9
(-1.1) 		1.5
(-3) 		3.7
(-1.8) 		12.1
(3) 		10.6
(2.2) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 0.7
(-2.6)
A 		1.2
(-1.6)
A 		3.6
(3)
A 		0.5
(-3)
A 		3
B 5.9
(3)
A 		0.7
(-2.6)
A 		0.9
(-2.4)
A 		0.3
(-3)
A 		6
C 2.5
(-0.5)
A 		1.4
(-2)
A 		0.7
(-3)
A 		5
(3)
A 		4
avr 3
(3) 		1.1
(-3) 		1.7
(-1.1) 		1.9
(-0.5) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.5
(-2.7)
A 		0.4
(-2.8)
A 		0.2
(-3)
A 		1
(-2.2)
A 		6.1
(3)
A 		0.6
(-2.6)
A 		6
B 0.6
(-2.4)
A 		0.5
(-2.6)
A 		0.3
(-3)
A 		1.1
(-1.4)
A 		3.3
(3)
A 		2.6
(1.6)
A 		3
C 0.5
(-3)
A 		3.7
(-0.3)
A 		0.6
(-2.9)
A 		0.5
(-3)
A 		2.6
(-1.2)
A 		7.6
(3)
A 		7
avr 0.5
(-2.8) 		1.5
(-1.2) 		0.4
(-3) 		0.9
(-2.2) 		4
(3) 		3.6
(2.3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181853_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5105
Sequence Type Exemplar sequence
Representative Public ID B0281.4 NCBI
Target Description B0281.4 /REP_DB=WormBase Gene ID /WP=CE07715 /TR=O16611 /GB=AAB66083.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061828(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061828 NCBI Caenorhabditis elegans B0281.4 (B0281.4) mRNA, complete cds. 10/11 None
SNAP00000033214 ENSEMBL cdna:SNAP chromosome:CEL140:II:2297939:2300819:-1 10/11 None
GENEFINDER00000033223 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2297939:2300819:-1 11/11 None
B0281.4 ENSEMBL cdna:known chromosome:CEL140:II:2297939:2298794:-1 gene:B0281.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2297937-2298817(-) 97.49 100.0
Public Domain and Genome References
Gene Title
Ensembl B0281.4
Entrez Gene 181898 Entrez gene
SwissProt O16611 EMBL-EBI
WormBase CE32096 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66084 Hypothetical protein B0281.5 [Caenorhabditis elegans] ref|NP_494230.1| B0281.5 [Caenorhabditis elegans] 1.0E-112
blast AAB66084 Hypothetical protein B0281.5 [Caenorhabditis elegans] ref|NP_494230.1| B0281.5 [Caenorhabditis elegans] 1.0E-109
blast AAB66083 Hypothetical protein B0281.4 [Caenorhabditis elegans] ref|NP_494229.2| B0281.4 [Caenorhabditis elegans] 1.0E-66
blast AAB66083 Hypothetical protein B0281.4 [Caenorhabditis elegans] ref|NP_494229.2| B0281.4 [Caenorhabditis elegans] 2.0E-65
blast AAB66083 Hypothetical protein B0281.4 [Caenorhabditis elegans] ref|NP_494229.2| B0281.4 [Caenorhabditis elegans] 9.0E-60
blast AAC68762 Hypothetical protein C40A11.7 [Caenorhabditis elegans] ref|NP_494199.1| C40A11.7 [Caenorhabditis elegans] 2.0E-43
blast AAB66084 Hypothetical protein B0281.5 [Caenorhabditis elegans] ref|NP_494230.1| B0281.5 [Caenorhabditis elegans] 3.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.7E-6
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.6E-46
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 6.5E-13
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.1E-13
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 6.5E-13
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.1E-5
Sequence
Target Sequence 
>C. ELEGANS:181853_AT
agcacgacggattcttcaaggcgctggttgagactgatattccggtgagttattttttgc
tgacacatcacgaatcggagaacgagattctcgaactgaagaaggaggctgaatactact
tgctgaacggcttggctgatttgtgtgagtatcagcgtccggtggacaacttcagaactt
gcactgctgatgagctgatgcgtgtcatcgtgaacaccaagaagaaagttattgtgatca
actacttgactcacgaggatcggctcgtctttgtcccaaccggcttcaacttttgcgatt
ttatggagcgtcacaaggataaggtggaagtcgtctttttcaataagttggaaaccgaat
actcgaacacagctagcgttccgcctcacattcacgatgtctgctggaggttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCACGACGGATTCTTCAAGGCGCT 269 77 83 Antisense
GGCGCTGGTTGAGACTGATATTCCG 271 543 102 Antisense
GAGTTATTTTTTGCTGACACATCAC 193 399 129 Antisense
ACTACTTGCTGAACGGCTTGGCTGA 127 101 197 Antisense
TTTGTGTGAGTATCAGCGTCCGGTG 278 665 222 Antisense
CGTCCGGTGGACAACTTCAGAACTT 426 245 238 Antisense
GAACTTGCACTGCTGATGAGCTGAT 463 349 257 Antisense
GCTGATGCGTGTCATCGTGAACACC 40 297 276 Antisense
CACGAGGATCGGCTCGTCTTTGTCC 34 201 334 Antisense
TACTCGAACACAGCTAGCGTTCCGC 26 647 442 Antisense
CATTCACGATGTCTGCTGGAGGTTC 358 213 471 Antisense
Sequence Source Affymetrix Proprietary Database