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[181849_at]

Description C53D5.3 /REP_DB=WormBase Gene ID /WP=CE20607 /TR=Q9N5V8 /GB=AAF39785.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 09      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_total > 19      4grp_total > 43      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 138.2
(-3)
P 		231.4
(3)
P 		165
(-1.3)
P 		178.6
(-0.4)
P 		93
B 191.6
(-1.3)
P 		297
(3)
P 		175.5
(-1.9)
P 		148.6
(-3)
P 		148
C 169.3
(-1.6)
P 		284.1
(3)
P 		135.8
(-3)
P 		214.8
(0.2)
P 		148
avr 166.4
(-2.6) 		270.8
(3) 		158.8
(-3) 		180.7
(-1.8) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 173
(0.5)
P 		174.9
(0.7)
P 		140.5
(-2.3)
P 		132.3
(-3)
P 		201.4
(3)
P 		153.9
(-1.1)
P 		69
B 196.3
(2.7)
P 		184.4
(1.9)
P 		190.8
(2.3)
P 		201.5
(3)
P 		197.7
(2.7)
P 		111.7
(-3)
P 		90
C 240.9
(3)
P 		124.5
(-3)
A 		203.9
(1.1)
P 		178.9
(-0.2)
M 		214.9
(1.7)
P 		124.8
(-3)
P 		116
avr 203.4
(2.9) 		161.3
(-0.5) 		178.4
(0.9) 		170.9
(0.3) 		204.7
(3) 		130.1
(-3) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 50.3
(3)
P 		50.2
(3)
P 		43.1
(1.2)
P 		26.1
(-3)
P 		24
B 61.1
(1.2)
P 		73.4
(3)
P 		40.7
(-1.7)
P 		31.7
(-3)
P 		42
C 56
(-0.4)
P 		81.7
(3)
P 		47.3
(-1.5)
P 		36.2
(-3)
P 		46
avr 55.8
(1) 		68.4
(3) 		43.7
(-1) 		31.3
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 31.9
(-2.8)
P 		62.2
(3)
P 		30.6
(-3)
P 		33.6
(-2.4)
P 		60.8
(2.7)
P 		37.5
(-1.7)
P 		32
B 68.3
(1.1)
P 		61.4
(0.1)
P 		46.3
(-2.3)
P 		53.4
(-1.2)
P 		80.3
(3)
P 		41.7
(-3)
P 		39
C 89.3
(3)
P 		38
(-2.8)
A 		51.9
(-1.2)
P 		36
(-3)
M 		69.5
(0.8)
P 		41.8
(-2.3)
P 		53
avr 63.2
(1.6) 		53.9
(-0.3) 		42.9
(-2.5) 		41
(-2.9) 		70.2
(3) 		40.3
(-3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181849_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2594
Sequence Type Exemplar sequence
Representative Public ID C53D5.3 NCBI
Target Description C53D5.3 /REP_DB=WormBase Gene ID /WP=CE20607 /TR=Q9N5V8 /GB=AAF39785.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058308(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058308 NCBI Caenorhabditis elegans C53D5.3 (C53D5.3) mRNA, complete cds. 11/11 None
SNAP00000029139 ENSEMBL cdna:SNAP chromosome:CEL140:I:280120:282374:1 11/11 None
GENEFINDER00000029151 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:280120:280762:1 11/11 None
C53D5.3 ENSEMBL cdna:known chromosome:CEL140:I:280120:280445:1 gene:C53D5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:280119-280445(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C53D5.3
Entrez Gene 183757 Entrez gene
SwissProt Q9N5V8 EMBL-EBI
WormBase CE20607 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39785 Hypothetical protein C53D5.3 [Caenorhabditis elegans] ref|NP_490709.1| C53D5.3 [Caenorhabditis elegans] 1.0E-23
blast AAF39785 Hypothetical protein C53D5.3 [Caenorhabditis elegans] ref|NP_490709.1| C53D5.3 [Caenorhabditis elegans] 3.0E-22
blast AAF39785 Hypothetical protein C53D5.3 [Caenorhabditis elegans] ref|NP_490709.1| C53D5.3 [Caenorhabditis elegans] 4.0E-21
blast CAE74311 Hypothetical protein CBG22020 [Caenorhabditis briggsae] 3.0E-10
blast CAE74311 Hypothetical protein CBG22020 [Caenorhabditis briggsae] 1.0E-9
blast CAE74310 Hypothetical protein CBG22019 [Caenorhabditis briggsae] 2.0E-9
blast CAE74311 Hypothetical protein CBG22020 [Caenorhabditis briggsae] 4.0E-9
Sequence
Target Sequence 
>C. ELEGANS:181849_AT
atggctcaggacggagaccgccatcacatttttgtcagtgctccgccgccgccgccacct
tcctcgtcgattccagctccatcgcagccaaatagactggaaaaggagccgctcatcgat
gaggaaactgacatgattgacgaatcccgagccacgtattggaagggatgtgaattcttg
aaagcctctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCTCAGGACGGAGACCGCCATC 163 53 13 Antisense
AGACCGCCATCACATTTTTGTCAGT 385 71 27 Antisense
ATCACATTTTTGTCAGTGCTCCGCC 8 29 35 Antisense
CCAGCTCCATCGCAGCCAAATAGAC 237 273 85 Antisense
GCTCCATCGCAGCCAAATAGACTGG 140 299 88 Antisense
TAGACTGGAAAAGGAGCCGCTCATC 13 653 105 Antisense
GGAGCCGCTCATCGATGAGGAAACT 56 523 117 Antisense
GAGGAAACTGACATGATTGACGAAT 282 405 133 Antisense
ACATGATTGACGAATCCCGAGCCAC 687 105 143 Antisense
GAATCCCGAGCCACGTATTGGAAGG 410 327 154 Antisense
GGATGTGAATTCTTGAAAGCCTCTG 289 515 178 Antisense
Sequence Source Affymetrix Proprietary Database