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[181848_at]

Description F25G6.2 /REP_DB=WormBase Gene ID /WP=CE09638 /TR=O16929 /GB=AAC25795.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 35      4grp_high_8_8 > 08      6grp_total > 35      4grp_total > 31      6grp_high1_sub > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 200.8
(3)
P 		85.1
(-3)
P 		142.4
(-0)
P 		128.2
(-0.8)
M 		116
B 202.4
(3)
P 		116.6
(-3)
P 		167.8
(0.6)
P 		166
(0.5)
P 		86
C 194.7
(3)
P 		174.8
(1.5)
P 		117.4
(-3)
M 		170.6
(1.1)
P 		77
avr 199.3
(3) 		125.5
(-3) 		142.5
(-1.6) 		154.9
(-0.6) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 170.8
(-0.1)
P 		247.5
(3)
P 		120.2
(-2.1)
P 		138.2
(-1.4)
P 		98.7
(-3)
P 		114.6
(-2.4)
P 		149
B 120.9
(-2.4)
P 		179.7
(3)
P 		148.5
(0.1)
P 		121.8
(-2.3)
M 		114.4
(-3)
P 		151.7
(0.4)
P 		65
C 215.9
(3)
P 		116.9
(-2.2)
P 		176.3
(0.9)
A 		105.7
(-2.8)
M 		102.2
(-3)
A 		121.5
(-2)
P 		114
avr 169.2
(2) 		181.4
(3) 		148.3
(0.4) 		121.9
(-1.7) 		105.1
(-3) 		129.3
(-1.1) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 73.1
(3)
P 		18.5
(-3)
P 		37.2
(-0.9)
P 		18.8
(-3)
M 		55
B 64.5
(3)
P 		28.8
(-3)
P 		38.9
(-1.3)
P 		35.4
(-1.9)
P 		36
C 64.5
(3)
P 		50.2
(0.6)
P 		40.9
(-1)
M 		28.8
(-3)
P 		36
avr 67.4
(3) 		32.5
(-2.3) 		39
(-1.3) 		27.7
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 31.5
(-2.5)
P 		88
(3)
P 		26.2
(-3)
P 		35
(-2.1)
P 		29.8
(-2.7)
P 		27.9
(-2.8)
P 		62
B 42.1
(-0.9)
P 		59.8
(3)
P 		36.1
(-2.2)
P 		32.3
(-3)
M 		46.5
(0.1)
P 		56.6
(2.3)
P 		28
C 80
(3)
P 		35.7
(-1.5)
P 		44.9
(-0.6)
A 		21.3
(-3)
M 		33.1
(-1.8)
A 		40.7
(-1)
P 		59
avr 51.2
(1.1) 		61.2
(3) 		35.7
(-1.8) 		29.5
(-3) 		36.5
(-1.7) 		41.7
(-0.7) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181848_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11334
Sequence Type Exemplar sequence
Representative Public ID F25G6.2 NCBI
Target Description F25G6.2 /REP_DB=WormBase Gene ID /WP=CE09638 /TR=O16929 /GB=AAC25795.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072809
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072809 NCBI Caenorhabditis elegans F25G6.2 (F25G6.2) mRNA, complete cds. None
GENEFINDER00000003994 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8572899:8576960:1 None
SNAP00000003983 ENSEMBL cdna:SNAP chromosome:CEL140:V:8572899:8577138:1 None
F25G6.2 ENSEMBL cdna:known chromosome:CEL140:V:8572899:8583994:1 gene:F25G6.2 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8572899-8577139(+) 98.82 100.0
Public Domain and Genome References
Gene Title
Ensembl F25G6.2
Entrez Gene 182490 Entrez gene
SwissProt O16929 EMBL-EBI
WormBase CE30764 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251115_AT expressed protein at
CANINE_2:CFAAFFX.7490.1.S1_S_AT similar to Symplekin cfa
DROSOPHILA_2:1630794_AT dm
DROSGENOME1:155136_AT dm
HG-U133A:32402_S_AT symplekin hs
HG-U133A_2:32402_S_AT symplekin hs
HG-FOCUS:32402_S_AT symplekin hs
HG-U133_PLUS_2:32402_S_AT symplekin hs
HG-U95AV2:32402_S_AT symplekin hs
HU35KSUBA:U49240_AT symplekin hs
HU35KSUBC:RC_F10446_F_AT symplekin hs
HU35KSUBD:RC_AA401297_S_AT symplekin hs
HUGENEFL:U88726_AT symplekin hs
HG-U133A:202339_AT symplekin hs
HG-U133_PLUS_2:202339_AT symplekin hs
HG-U133A_2:202339_AT symplekin hs
U133_X3P:G4759195_3P_S_AT symplekin hs
U133_X3P:G4759195_3P_AT symplekin hs
U133_X3P:HS2.378736.1.S1_3P_X_AT symplekin hs
U133_X3P:HS2.378736.1.S1_3P_AT symplekin hs
U133_X3P:HS2.107019.2.S1_3P_AT symplekin hs
U133_X3P:202339_3P_AT symplekin hs
HG-U133_PLUS_2:1554595_AT symplekin hs
HG-U133_PLUS_2:1559587_AT symplekin hs
HG-U95B:52396_S_AT symplekin hs
MG-U74BV2:110846_AT symplekin mm
MG-U74CV2:171079_AT symplekin mm
MOE430B:1428673_AT symplekin mm
MOUSE430_2:1428673_AT symplekin mm
MU19KSUBA:TC20304_AT symplekin mm
MOE430B:1436901_AT PREDICTED: symplekin [Mus musculus], mRNA sequence mm
MOUSE430_2:1436901_AT PREDICTED: symplekin [Mus musculus], mRNA sequence mm
MOE430B:1459000_AT PREDICTED: symplekin [Mus musculus], mRNA sequence mm
MOUSE430_2:1459000_AT PREDICTED: symplekin [Mus musculus], mRNA sequence mm
MU19KSUBB:TC27354_AT PREDICTED: symplekin [Mus musculus], mRNA sequence mm
MU19KSUBC:TC35654_AT PREDICTED: symplekin [Mus musculus], mRNA sequence mm
RAE230A:1374733_AT symplekin (predicted) rn
RAT230_2:1374733_AT symplekin (predicted) rn
RAE230B:1397651_AT Symplekin (predicted) rn
RAT230_2:1397651_AT Symplekin (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC25795 Hypothetical protein F25G6.2 [Caenorhabditis elegans] ref|NP_505210.2| F25G6.2 [Caenorhabditis elegans] 0.0
blast CAE58364 Hypothetical protein CBG01489 [Caenorhabditis briggsae] 0.0
blast Q92797 Symplekin 1.0E-105
Sequence
Target Sequence 
>C. ELEGANS:181848_AT
attgttgaagccagataccgcatcccaagctatcgaagctgttttcaaatgggaaaatgt
tccatttctatttctacactctctttatactctttttcataagttcaaaacattcgaatc
gttcgtcgcaaatctgttctacaaagtaactgaaaagaagatgtatcaacaatcggatcg
atggaagcaggcatttttcaaatgtatcaaagaattgaaaaccaaagcgtatccagcggt
tatcacatttttgagttttgaagaatacgaagagctaaaggaagttttaggagatggaat
tgtcgcagaattcaaaataatttattcaacacttgcaacacaacagcagaagaatatgga
cgagaaaatcaaagaggagttgcatgataaagaacgagaaaatcgtgaacgagataagcg
acttcgaagagaagagaaaaaggaaaaggaaagagaaaaggaacgaactcgcgaaagtgg
aaaggaaagactaaaaacctccaattttgtcaatttttctgttttgaagcggtcgaaaac
ggtgctgaatccaaagtttttcttcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGTTGAAGCCAGATACCGCATCC 307 3 2940 Antisense
TACCGCATCCCAAGCTATCGAAGCT 545 647 2955 Antisense
ATGTTCCATTTCTATTTCTACACTC 563 47 2996 Antisense
CACTCTCTTTATACTCTTTTTCATA 50 199 3016 Antisense
TCGTTCGTCGCAAATCTGTTCTACA 670 603 3058 Antisense
GGATCGATGGAAGCAGGCATTTTTC 426 511 3114 Antisense
AAGCGTATCCAGCGGTTATCACATT 3 149 3164 Antisense
TATTCAACACTTGCAACACAACAGC 346 663 3262 Antisense
ACCTCCAATTTTGTCAATTTTTCTG 333 81 3436 Antisense
GCGGTCGAAAACGGTGCTGAATCCA 600 291 3468 Antisense
GCTGAATCCAAAGTTTTTCTTCGAA 145 297 3483 Antisense
Sequence Source Affymetrix Proprietary Database