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[181827_at]

Description F45E1.3 /REP_DB=WormBase Gene ID /WP=CE10482 /TR=Q20426 /GB=AAB04900.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 72      4grp_highest_8_8 > 06      4grp_high_8_8 > 22      6grp_highest_nonorm > 54      6grp_total > 73      4grp_total > 25      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1559
1 2 3 4 range
A 278.3
(-3)
P 		988
(-0.2)
P 		986.5
(-0.2)
P 		1806.1
(3)
P 		1528
B 275.6
(-3)
P 		951.9
(-0.3)
P 		953.8
(-0.3)
P 		1795.1
(3)
P 		1520
C 246.7
(-3)
P 		989.6
(-0.1)
P 		1017.8
(-0)
P 		1801.2
(3)
P 		1555
avr 266.9
(-3) 		976.5
(-0.2) 		986
(-0.2) 		1800.8
(3) 		1534

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1040
1 2 3 4 5 6 range
A 558.3
(-3)
P 		800.8
(-1.5)
P 		1523.7
(3)
P 		1378.9
(2.1)
P 		1333.3
(1.8)
P 		860.7
(-1.1)
P 		965
B 486.9
(-3)
P 		834.2
(-0.9)
P 		1451.4
(2.9)
P 		1459.7
(3)
P 		1218.6
(1.5)
P 		846.1
(-0.8)
P 		973
C 483.6
(-3)
P 		892.2
(-0.5)
P 		1426
(2.8)
P 		1460.8
(3)
P 		1199.9
(1.4)
P 		781.8
(-1.2)
P 		977
avr 509.6
(-3) 		842.4
(-0.9) 		1467
(3) 		1433.1
(2.8) 		1250.6
(1.6) 		829.5
(-1) 		957
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 101.3
(-3)
P 		214.5
(1.2)
P 		257.9
(2.8)
P 		264.4
(3)
P 		163
B 87.9
(-3)
P 		235.1
(-0)
P 		221
(-0.3)
P 		382.5
(3)
P 		295
C 81.7
(-3)
P 		284.5
(1.5)
P 		354.7
(3)
P 		303.7
(1.9)
P 		273
avr 90.3
(-3) 		244.7
(1.1) 		277.9
(2) 		316.9
(3) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 102.9
(-3)
P 		284.8
(0.6)
P 		331.8
(1.6)
P 		349.7
(1.9)
P 		402.3
(3)
P 		209.5
(-0.9)
P 		299
B 169.4
(-3)
P 		277.7
(-1)
P 		352.5
(0.4)
P 		386.6
(1)
P 		495.1
(3)
P 		315.5
(-0.3)
P 		326
C 179.3
(-3)
P 		272.2
(-0.3)
P 		363
(2.3)
P 		293.8
(0.3)
P 		388.3
(3)
P 		261.7
(-0.6)
P 		209
avr 150.5
(-3) 		278.2
(-0.2) 		349.1
(1.3) 		343.4
(1.2) 		428.6
(3) 		262.2
(-0.6) 		278
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181827_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16658
Sequence Type Exemplar sequence
Representative Public ID F45E1.3 NCBI
Target Description F45E1.3 /REP_DB=WormBase Gene ID /WP=CE10482 /TR=Q20426 /GB=AAB04900.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076945(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076945 NCBI Caenorhabditis elegans F45E1.3 (F45E1.3) mRNA, complete cds. 11/11 None
GENEFINDER00000004158 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7982559:7988750:1 11/11 None
SNAP00000004149 ENSEMBL cdna:SNAP chromosome:CEL140:X:7987476:7988750:1 11/11 None
F45E1.3 ENSEMBL cdna:known chromosome:CEL140:X:7986599:7988750:1 gene:F45E1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7986597-7988749(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45E1.3
Entrez Gene 185792 Entrez gene
SwissProt Q20426 EMBL-EBI
WormBase CE10482 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB04900 Hypothetical protein F45E1.3 [Caenorhabditis elegans] ref|NP_509346.1| F45E1.3 [Caenorhabditis elegans] 1.0E-145
blast AAB04900 Hypothetical protein F45E1.3 [Caenorhabditis elegans] ref|NP_509346.1| F45E1.3 [Caenorhabditis elegans] 1.0E-144
blast AAB04900 Hypothetical protein F45E1.3 [Caenorhabditis elegans] ref|NP_509346.1| F45E1.3 [Caenorhabditis elegans] 1.0E-116
blast CAE70331 Hypothetical protein CBG16864 [Caenorhabditis briggsae] 3.0E-98
blast CAE70331 Hypothetical protein CBG16864 [Caenorhabditis briggsae] 4.0E-98
blast CAE70331 Hypothetical protein CBG16864 [Caenorhabditis briggsae] 6.0E-98
blast CAB07180 Hypothetical protein F09C3.2 [Caenorhabditis elegans] ref|NP_493493.2| F09C3.2 [Caenorhabditis elegans] 1.0E-31
blast CAB07180 Hypothetical protein F09C3.2 [Caenorhabditis elegans] ref|NP_493493.2| F09C3.2 [Caenorhabditis elegans] 3.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.3E-20
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.3E-20
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 3.4E-20
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509346.1 1 15-34
F45E1.3 1 15-34
GENEFINDER00000004158 2 119-137,147-166
Sequence
Target Sequence 
>C. ELEGANS:181827_AT
tcgactggacttgtgctattcccaaaaattgcccaatctctaggactgtgcttagccgag
aataaagtgaaaccgggtcttcttgcggaaggaaccaccggtcaaattgcaaatgaaatc
tcatctcttctcatggataatggaataaaatcttttgaagcacgcgaaataacaaataac
tccttgaccaattcgtacgataaaattctttccaccgatcttgtcaaggagctcgccgat
acagttgccctcttcacaagattaaaacaacatgggacaaaaattgcggtctgtacagca
gataatagaaaaagttccctccttgctctgaaacgtatgaatgttgatcatttagtcgat
atgattgtttgcggtgacgacaaaaacactgctccaaaaccttctcctcataatgccata
aaaatttgcaaacatcttggtgtcgatcaatcgaaagcgataatggttggagacactcga
gttgatatggaaatggctcacaatgctgaactcggagcagctgttggagtactctccgga
attggctgcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGACTGGACTTGTGCTATTCCCAA 367 601 160 Antisense
TGCCCAATCTCTAGGACTGTGCTTA 28 585 189 Antisense
GTGAAACCGGGTCTTCTTGCGGAAG 198 447 226 Antisense
GAAATCTCATCTCTTCTCATGGATA 282 363 274 Antisense
ATAACTCCTTGACCAATTCGTACGA 516 23 335 Antisense
TAAAATTCTTTCCACCGATCTTGTC 443 639 360 Antisense
CAAGGAGCTCGCCGATACAGTTGCC 261 183 384 Antisense
TACAGTTGCCCTCTTCACAAGATTA 403 643 399 Antisense
TCCCTCCTTGCTCTGAAACGTATGA 181 597 475 Antisense
AACCTTCTCCTCATAATGCCATAAA 466 147 557 Antisense
AGTACTCTCCGGAATTGGCTGCAAA 671 61 687 Antisense
Sequence Source Affymetrix Proprietary Database