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[181821_at]

Description F20A1.6 /REP_DB=WormBase Gene ID /WP=CE27982 /TR=Q19610 /GB=AAA96126.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 88      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 99      6grp_total > 87      4grp_total > 02      6grp_highest_sub > In      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7190
1 2 3 4 range
A 747.9
(-2.5)
P 		216
(-2.9)
P 		126.5
(-3)
P 		7307.1
(3)
P 		7181
B 583.5
(-2.6)
P 		124.1
(-3)
P 		117
(-3)
P 		7043
(3)
P 		6926
C 512.7
(-2.7)
P 		294.2
(-2.9)
P 		241.1
(-3)
P 		5921
(3)
P 		5680
avr 614.7
(-2.6) 		211.4
(-3) 		161.5
(-3) 		6757
(3) 		6596

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5054
1 2 3 4 5 6 range
A 463.8
(-2.9)
P 		396.4
(-3)
P 		870.8
(-2.3)
P 		817.5
(-2.3)
P 		2206.2
(-0.1)
P 		4205.2
(3)
P 		3809
B 413.1
(-3)
P 		493.8
(-2.9)
P 		629.4
(-2.7)
P 		485.2
(-2.9)
P 		2541.1
(-0.4)
P 		5401
(3)
P 		4988
C 346.9
(-3)
P 		578.4
(-2.7)
P 		822.2
(-2.4)
P 		491.1
(-2.8)
P 		2174.6
(-0.8)
P 		5235
(3)
P 		4888
avr 407.9
(-3) 		489.5
(-2.9) 		774.1
(-2.5) 		597.9
(-2.7) 		2307.3
(-0.5) 		4947.1
(3) 		4539
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1474
1 2 3 4 range
A 272.3
(-1.6)
P 		46.9
(-2.9)
P 		33.1
(-3)
P 		1069.6
(3)
P 		1037
B 186.1
(-2.4)
P 		30.7
(-3)
P 		27.1
(-3)
P 		1500.9
(3)
P 		1474
C 169.7
(-2.4)
P 		84.6
(-3)
P 		84
(-3)
P 		998.4
(3)
P 		914
avr 209.4
(-2.2) 		54.1
(-3) 		48.1
(-3) 		1189.6
(3) 		1142

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1929
1 2 3 4 5 6 range
A 85.5
(-3)
P 		141
(-2.6)
P 		189.7
(-2.3)
P 		207.3
(-2.2)
P 		665.7
(0.7)
P 		1023.8
(3)
P 		938
B 143.7
(-3)
P 		164.4
(-2.9)
P 		152.8
(-2.9)
P 		128.5
(-3)
P 		1032.4
(-0.1)
P 		2014
(3)
P 		1886
C 128.6
(-2.9)
P 		176.5
(-2.7)
P 		209.3
(-2.6)
P 		98.8
(-3)
P 		703.7
(-0.8)
P 		1752.5
(3)
P 		1654
avr 119.3
(-3) 		160.6
(-2.8) 		183.9
(-2.7) 		144.9
(-2.9) 		800.6
(-0.2) 		1596.8
(3) 		1478
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181821_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11442
Sequence Type Exemplar sequence
Representative Public ID F20A1.6 NCBI
Target Description F20A1.6 /REP_DB=WormBase Gene ID /WP=CE27982 /TR=Q19610 /GB=AAA96126.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028649, NM_001028648
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000026817 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7028224:7029625:-1 None
NM_001028648 NCBI Caenorhabditis elegans F20A1.6a (F20A1.6) mRNA, complete cds. None
NM_001028649 NCBI Caenorhabditis elegans F20A1.6b (F20A1.6) mRNA, complete cds. None
F20A1.6a ENSEMBL cdna:known chromosome:CEL140:V:7027225:7029676:-1 gene:F20A1.6 A 
F20A1.6b ENSEMBL cdna:known chromosome:CEL140:V:7027380:7029673:-1 gene:F20A1.6 A 
SNAP00000026799 ENSEMBL cdna:SNAP chromosome:CEL140:V:7029008:7029673:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7027983-7029674(-) 100.0 100.0
Public Domain and Genome References
Ensembl F20A1.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023819 F20A1.6a [Caenorhabditis elegans] gb|AAA96126.4| Hypothetical protein F20A1.6a [Caenorhabditis elegans] 1.0E-101
blast NP_001023820 F20A1.6b [Caenorhabditis elegans] gb|AAT81197.1| Hypothetical protein F20A1.6b [Caenorhabditis elegans] 3.0E-86
blast NP_001023819 F20A1.6a [Caenorhabditis elegans] gb|AAA96126.4| Hypothetical protein F20A1.6a [Caenorhabditis elegans] 1.0E-80
blast NP_001023820 F20A1.6b [Caenorhabditis elegans] gb|AAT81197.1| Hypothetical protein F20A1.6b [Caenorhabditis elegans] 2.0E-80
blast NP_001023819 F20A1.6a [Caenorhabditis elegans] gb|AAA96126.4| Hypothetical protein F20A1.6a [Caenorhabditis elegans] 4.0E-56
blast CAE72039 Hypothetical protein CBG19121 [Caenorhabditis briggsae] 3.0E-49
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023819.1 1 7-24
NP_001023820.1 1 7-24
F20A1.6a 1 7-24
F20A1.6b 1 7-24
SNAP00000026799 1 7-24
Sequence
Target Sequence 
>C. ELEGANS:181821_AT
tgatggttggaatggcggcagccgttgagattcagaattctggatcgtttgctactggta
ctgactaccagaaggaaaaagaacgacagggacaagacccgaacaactataagaaaacgt
atcataaagaaattcctggaggatcggaacatgcagaggttgttgcttcaaatgaaccag
gttcaaactcgtggagtcagacatacagcaaacaccaatcgttcggttcttcagacactc
aacaatctcaagaactgaatcaaaggttggcgatgatgcttaagactaacacccgtcact
attgcaccttgtatagtctctgtgtgtttcgtcgaacgtcgttcactttcccaagttcac
tgaaatctaataaacgtgtttatgtttctcttttgttttctaagcatgtcacttcacag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGGTTGGAATGGCGGCAGCCGT 466 565 50 Antisense
TCTGGATCGTTTGCTACTGGTACTG 471 605 88 Antisense
GAACCAGGTTCAAACTCGTGGAGTC 422 345 223 Antisense
GCAAACACCAATCGTTCGGTTCTTC 531 317 257 Antisense
GATGCTTAAGACTAACACCCGTCAC 672 411 324 Antisense
CCCGTCACTATTGCACCTTGTATAG 281 259 341 Antisense
TATAGTCTCTGTGTGTTTCGTCGAA 664 659 361 Antisense
TCGTCGAACGTCGTTCACTTTCCCA 367 603 378 Antisense
GTTCACTTTCCCAAGTTCACTGAAA 96 445 390 Antisense
TGTTTATGTTTCTCTTTTGTTTTCT 305 561 426 Antisense
GTTTTCTAAGCATGTCACTTCACAG 657 447 444 Antisense
Sequence Source Affymetrix Proprietary Database