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[181817_s_at]

Description C32E8.9 /REP_DB=WormBase Gene ID /WP=CE08533 /TR=P91130 /GB=AAB42326.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 52      6grp_total > 50      4grp_total > 62      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 450
1 2 3 4 range
A 616.8
(3)
P 		391.8
(-0)
P 		375.7
(-0.3)
P 		173.7
(-3)
P 		443
B 619.9
(3)
P 		423.3
(0.4)
P 		458.4
(0.8)
P 		169.9
(-3)
P 		450
C 590.2
(3)
P 		336.8
(-1)
P 		427.4
(0.4)
P 		207.7
(-3)
P 		383
avr 609
(3) 		384
(-0.2) 		420.5
(0.3) 		183.8
(-3) 		425

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 570
1 2 3 4 5 6 range
A 512.9
(-0.3)
P 		522.4
(-0.2)
P 		742.6
(3)
P 		706.9
(2.5)
P 		328.5
(-3)
P 		428.9
(-1.5)
P 		414
B 459.3
(-2.2)
P 		537.3
(-1.2)
P 		861.6
(3)
P 		722.4
(1.2)
P 		398.7
(-3)
P 		409.7
(-2.9)
P 		463
C 445.4
(-2.3)
P 		509.5
(-1.6)
P 		772.7
(1.5)
P 		898.9
(3)
P 		387.6
(-3)
P 		404.6
(-2.8)
P 		511
avr 472.5
(-1.6) 		523.1
(-0.8) 		792.3
(3) 		776.1
(2.8) 		371.6
(-3) 		414.4
(-2.4) 		421
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 199
1 2 3 4 range
A 224.6
(3)
P 		85.1
(-1.2)
P 		98.2
(-0.8)
P 		25.4
(-3)
P 		199
B 197.7
(3)
P 		104.5
(-0.5)
P 		106.2
(-0.4)
P 		36.2
(-3)
P 		162
C 195.4
(3)
P 		96.8
(-0.7)
P 		149
(1.3)
P 		35
(-3)
P 		160
avr 205.9
(3) 		95.5
(-0.8) 		117.8
(-0) 		32.2
(-3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 94.5
(-3)
P 		185.8
(3)
P 		161.7
(1.4)
P 		179.3
(2.6)
P 		99.1
(-2.7)
P 		104.4
(-2.3)
P 		91
B 159.8
(-2.3)
P 		178.8
(-0.2)
P 		209.2
(3)
P 		191.3
(1.1)
P 		162
(-2)
P 		152.8
(-3)
P 		56
C 165.1
(0.3)
P 		155.4
(-0.5)
P 		196.7
(3)
P 		180.8
(1.7)
P 		125.4
(-3)
P 		135.5
(-2.2)
P 		71
avr 139.8
(-1.9) 		173.3
(1.4) 		189.2
(3) 		183.8
(2.5) 		128.8
(-3) 		130.9
(-2.8) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181817_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.243
Sequence Type Exemplar sequence
Representative Public ID C32E8.9 NCBI
Target Description C32E8.9 /REP_DB=WormBase Gene ID /WP=CE08533 /TR=P91130 /GB=AAB42326.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058821(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058821 NCBI Caenorhabditis elegans C32E8.9 (C32E8.9) mRNA, complete cds. 11/11 None
SNAP00000040654 ENSEMBL cdna:SNAP chromosome:CEL140:I:3794630:3795600:-1 9/11 None
GENEFINDER00000040665 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3794630:3795921:-1 11/11 None
C32E8.9 ENSEMBL cdna:known chromosome:CEL140:I:3794623:3798055:-1 gene:C32E8.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3794629-3795611(-) 99.68 99.68
Public Domain and Genome References
Gene Title
Ensembl C32E8.9
Entrez Gene 171951 Entrez gene
SwissProt P91130 EMBL-EBI
WormBase CE29693 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.2482.1.S1_AT similar to enoyl Coenzyme A hydratase domain containing 1 cfa
CANINE_2:CFAAFFX.2482.1.S1_S_AT similar to enoyl Coenzyme A hydratase domain containing 1 cfa
HG-U133_PLUS_2:223088_X_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133B:223088_X_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133_PLUS_2:219974_X_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133A:219974_X_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133A_2:219974_X_AT enoyl Coenzyme A hydratase domain containing 1 hs
HU35KSUBA:RC_AA455175_AT enoyl Coenzyme A hydratase domain containing 1 hs
HU35KSUBA:N55978_AT enoyl Coenzyme A hydratase domain containing 1 hs
U133_X3P:G8923863_3P_X_AT enoyl Coenzyme A hydratase domain containing 1 hs
U133_X3P:G13097674_3P_A_AT enoyl Coenzyme A hydratase domain containing 1 hs
U133_X3P:HS.239218.2.A1_3P_A_AT enoyl Coenzyme A hydratase domain containing 1 hs
U133_X3P:HS.239218.1.A2_3P_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133_PLUS_2:223087_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133B:223087_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133_PLUS_2:233124_S_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133B:233124_S_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U95C:48928_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U95C:49255_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U95B:52381_S_AT enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133_PLUS_2:243303_AT Enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133B:243303_AT Enoyl Coenzyme A hydratase domain containing 1 hs
U133_X3P:HS.220913.0.A1_3P_AT Enoyl Coenzyme A hydratase domain containing 1 hs
HG-U95E:71634_AT Enoyl Coenzyme A hydratase domain containing 1 hs
HG-U133_PLUS_2:1563303_AT Enoyl Coenzyme A hydratase domain containing 1 hs
HG-U95D:86072_R_AT Enoyl Coenzyme A hydratase domain containing 1 hs
HU35KSUBA:AA478674_AT Enoyl Coenzyme A hydratase domain containing 1 hs
HU35KSUBB:RC_AA173573_AT Enoyl Coenzyme A hydratase domain containing 1 hs
MOUSE430_2:1425001_AT enoyl Coenzyme A hydratase domain containing 1 mm
MOUSE430A_2:1425001_AT enoyl Coenzyme A hydratase domain containing 1 mm
MOE430A:1425001_AT enoyl Coenzyme A hydratase domain containing 1 mm
MG-U74BV2:113214_AT enoyl Coenzyme A hydratase domain containing 1 mm
MOE430A:1419552_AT enoyl Coenzyme A hydratase domain containing 1 mm
MOUSE430A_2:1419552_AT enoyl Coenzyme A hydratase domain containing 1 mm
MOUSE430_2:1419552_AT enoyl Coenzyme A hydratase domain containing 1 mm
MU19KSUBB:TC31613_AT Enoyl Coenzyme A hydratase domain containing 1, mRNA (cDNA clone IMAGE:4016974) mm
MU19KSUBB:TC33692_AT Enoyl Coenzyme A hydratase domain containing 1, mRNA (cDNA clone IMAGE:4016974) mm
MU19KSUBC:TC38914_AT Enoyl Coenzyme A hydratase domain containing 1, mRNA (cDNA clone IMAGE:4016974) mm
MU19KSUBC:TC38914_G_AT Enoyl Coenzyme A hydratase domain containing 1, mRNA (cDNA clone IMAGE:4016974) mm
RG-U34B:RC_AA851921_AT enoyl Coenzyme A hydratase domain containing 1 rn
RAE230A:1374585_AT enoyl Coenzyme A hydratase domain containing 1 rn
RAT230_2:1374585_AT enoyl Coenzyme A hydratase domain containing 1 rn
RAE230B:1394971_AT Enoyl Coenzyme A hydratase domain containing 1 rn
RAT230_2:1394971_AT Enoyl Coenzyme A hydratase domain containing 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42326 Hypothetical protein C32E8.9 [Caenorhabditis elegans] ref|NP_491222.2| C32E8.9 [Caenorhabditis elegans] 1.0E-138
blast CAE66668 Hypothetical protein CBG12007 [Caenorhabditis briggsae] 1.0E-112
blast AAB42326 Hypothetical protein C32E8.9 [Caenorhabditis elegans] ref|NP_491222.2| C32E8.9 [Caenorhabditis elegans] 1.0E-101
blast CAE66668 Hypothetical protein CBG12007 [Caenorhabditis briggsae] 4.0E-86
blast AAB42326 Hypothetical protein C32E8.9 [Caenorhabditis elegans] ref|NP_491222.2| C32E8.9 [Caenorhabditis elegans] 6.0E-84
blast CAE66668 Hypothetical protein CBG12007 [Caenorhabditis briggsae] 5.0E-71
blast AAH88780 LOC496886 protein [Xenopus tropicalis] 6.0E-24
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 2.4E-11
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 3.5E-13
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 3.3E-17
Sequence
Target Sequence 
>C. ELEGANS:181817_S_AT
ttggaaaatcgttctgctcgggtgctgatttgggtcttatcaaagatatttcggaccaaa
agcttggcgtccaaatgttcgagtacatgtcatcaattctttcacttctccactcatcac
cagctatttcaattgcgaaaatccatggccacgcactcggcggagccaccgaaatttgct
cgtcgaccgacattcgaattgctcattctggctcgaaaatcgcattttttcaatcgaaaa
tgggaattgttccgtcgtggggaggtgccgaatatatggagggaattatgggtagaggac
gagcattagctgcaatgggacgggcaaatgttatgtcagctgaggaagcaaaggatcaag
gatatgtggattatgtgtacaaaagtgaagatgaagctgagaacttcatcaaccaagtgg
ctagcgctggtttgaaagtgacaagagcccagaaagctatgctgaacgctgtaaaaatag
gaaaaacggagcaaaaacaagtgctggaagccgtctggaacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGAAAATCGTTCTGCTCGGGTGC 160 709 59 Antisense
CTCGGGTGCTGATTTGGGTCTTATC 363 227 75 Antisense
GCTTGGCGTCCAAATGTTCGAGTAC 453 307 120 Antisense
CATGTCATCAATTCTTTCACTTCTC 84 211 144 Antisense
CACTCATCACCAGCTATTTCAATTG 561 195 169 Antisense
TGCGAAAATCCATGGCCACGCACTC 471 583 192 Antisense
GAGCCACCGAAATTTGCTCGTCGAC 458 385 221 Antisense
GCTCGTCGACCGACATTCGAATTGC 563 299 236 Antisense
GAATTGCTCATTCTGGCTCGAAAAT 413 323 254 Antisense
GAATTGTTCCGTCGTGGGGAGGTGC 607 323 302 Antisense
CAAGTGCTGGAAGCCGTCTGGAACG 638 183 556 Antisense
Sequence Source Affymetrix Proprietary Database