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[181811_at]

Description C54G7.4 /REP_DB=WormBase Gene ID /WP=CE04272 /TR=Q18859 /GB=AAA81394.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_high_8_8 > 26      6grp_highest_nonorm > 80      6grp_total > 26      4grp_total > 38      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 316
1 2 3 4 range
A 381.7
(3)
P 		180.6
(-1.3)
M 		99.1
(-3)
P 		245
(0.1)
P 		283
B 356.2
(3)
P 		274
(0.4)
P 		167.3
(-3)
P 		226.5
(-1.1)
P 		189
C 414.8
(3)
P 		221
(-1.3)
P 		143.9
(-3)
P 		269.4
(-0.2)
P 		271
avr 384.2
(3) 		225.2
(-0.9) 		136.8
(-3) 		247
(-0.3) 		247

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 310.9
(3)
P 		286.3
(1.9)
P 		171.9
(-3)
P 		197.1
(-1.9)
P 		179.6
(-2.7)
P 		223.2
(-0.8)
P 		139
B 358.9
(3)
P 		268.2
(-0.1)
P 		191.7
(-2.7)
P 		249.4
(-0.8)
P 		184.2
(-3)
P 		197.7
(-2.5)
P 		175
C 283.3
(2.9)
P 		284.5
(3)
P 		197.9
(-0.6)
P 		157
(-2.3)
P 		141.2
(-3)
P 		170.2
(-1.8)
P 		143
avr 317.7
(3) 		279.7
(1.5) 		187.2
(-2.2) 		201.2
(-1.7) 		168.3
(-3) 		197
(-1.8) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 139
(3)
P 		39.2
(-2.3)
M 		25.9
(-3)
P 		35.9
(-2.5)
P 		113
B 113.6
(3)
P 		67.7
(-0.7)
P 		38.8
(-3)
P 		48.3
(-2.2)
P 		75
C 137.3
(3)
P 		63.5
(-1.8)
P 		50.1
(-2.7)
P 		45.4
(-3)
P 		92
avr 130
(3) 		56.8
(-1.8) 		38.3
(-3) 		43.2
(-2.7) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 57.3
(-1.1)
P 		101.8
(3)
P 		37.4
(-3)
P 		50
(-1.8)
P 		54.2
(-1.4)
P 		54.3
(-1.4)
P 		64
B 124.9
(3)
P 		89.3
(0.3)
P 		46.5
(-3)
P 		66.1
(-1.5)
P 		74.8
(-0.8)
P 		73.7
(-0.9)
P 		78
C 105
(3)
P 		86.8
(1.5)
P 		50.4
(-1.5)
P 		31.6
(-3)
P 		45.7
(-1.8)
P 		57
(-0.9)
P 		73
avr 95.7
(3) 		92.6
(2.6) 		44.8
(-3) 		49.2
(-2.5) 		58.2
(-1.4) 		61.7
(-1) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181811_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16641
Sequence Type Exemplar sequence
Representative Public ID C54G7.4 NCBI
Target Description C54G7.4 /REP_DB=WormBase Gene ID /WP=CE04272 /TR=Q18859 /GB=AAA81394.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076539(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076539 NCBI Caenorhabditis elegans C54G7.4 (C54G7.4) mRNA, complete cds. 11/11 None
SNAP00000026248 ENSEMBL cdna:SNAP chromosome:CEL140:X:5545647:5548030:-1 11/11 None
GENEFINDER00000026253 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5545647:5552656:-1 11/11 None
C54G7.4 ENSEMBL cdna:known chromosome:CEL140:X:5545647:5552596:-1 gene:C54G7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5545646-5552596(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54G7.4
Entrez Gene 183817 Entrez gene
SwissProt Q18859 EMBL-EBI
WormBase CE37621 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.6650.1.S1_AT similar to WD repeat domain 35 cfa
CANINE_2:CFAAFFX.6650.1.S1_S_AT similar to WD repeat domain 35 cfa
DROSOPHILA_2:1629688_AT dm
DROSGENOME1:154491_AT dm
CHICKEN:GGA.10077.2.S1_S_AT similar to WD repeat domain 35 gga
CHICKEN:GGA.18413.1.S1_S_AT similar to WD repeat domain 35 gga
CHICKEN:GGAAFFX.24971.3.S1_S_AT similar to WD repeat domain 35 gga
HU35KSUBB:RC_Z38228_AT WD repeat domain 35 hs
U133_X3P:HS.145365.0.S2_3P_AT WD repeat domain 35 hs
U133_X3P:HS.145365.0.S1_3P_AT WD repeat domain 35 hs
HG-U133_PLUS_2:226889_AT WD repeat domain 35 hs
HG-U133B:226889_AT WD repeat domain 35 hs
HG-U133_PLUS_2:226890_AT WD repeat domain 35 hs
HG-U133B:226890_AT WD repeat domain 35 hs
HG-U95B:57507_AT WD repeat domain 35 hs
HG-U95E:82741_AT WD repeat domain 35 hs
HG-U95E:68588_AT WD repeat domain 35 hs
MG-U74BV2:113476_AT WD repeat domain 35 mm
MOE430B:1454790_AT WD repeat domain 35 mm
MOUSE430_2:1454790_AT WD repeat domain 35 mm
MOE430B:1440318_AT WD repeat domain 35 mm
MOUSE430_2:1440318_AT WD repeat domain 35 mm
Protein Similarities
Method ID Description E-Value
blast AAA81394 Hypothetical protein C54G7.4 [Caenorhabditis elegans] ref|NP_508940.3| C54G7.4 [Caenorhabditis elegans] 0.0
blast CAE68842 Hypothetical protein CBG14804 [Caenorhabditis briggsae] 0.0
blast XP_783217 PREDICTED: similar to WD repeat domain 35 [Strongylocentrotus purpuratus] 1.0E-179
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0084
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.34
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0084
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0090
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.34
Sequence
Target Sequence 
>C. ELEGANS:181811_AT
gaatccgggttgagtgctgagcagtcaagaattttggagaatacctggagaggagcagaa
gcttttcattttatgataatggcacaacaacactttttcgagaacagaatcaaagatgct
cttcaaacttccctgattttatctgattacgaagaatttcttgatccggcggaagttcac
tctatgatcgctctcgcagcagcaaatgtcaatcaattcggaatttgttccaaagcaatg
atgcgcttggaagcgtttgaggagtttgacgatgctgaaaaagaggagatgagaaacttg
tcgtttcgattgttctcagaaaatcctccagtgaaccccaactcggctaaagtagcttgc
tccgtatgtgatgctaagatcgatccgtatgacttacagtgcagcgaatgtcaaacaaag
tttcccgtgtgcattgcatctggaagattaatcctggacaatattttctggctgtgtcca
agatgtaagcatcgtgctcatcaacatgaaatacctaagtacaattgctgcccgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCCGGGTTGAGTGCTGAGCAGT 80 331 2803 Antisense
ATGGCACAACAACACTTTTTCGAGA 74 53 2881 Antisense
GCTCTTCAAACTTCCCTGATTTTAT 667 299 2920 Antisense
ATCCGGCGGAAGTTCACTCTATGAT 413 39 2966 Antisense
ATCGCTCTCGCAGCAGCAAATGTCA 636 37 2989 Antisense
TCAGAAAATCCTCCAGTGAACCCCA 673 621 3118 Antisense
TGAACCCCAACTCGGCTAAAGTAGC 224 573 3134 Antisense
AAGTAGCTTGCTCCGTATGTGATGC 579 157 3152 Antisense
GTTTCCCGTGTGCATTGCATCTGGA 62 451 3222 Antisense
TAAGCATCGTGCTCATCAACATGAA 394 637 3288 Antisense
TACCTAAGTACAATTGCTGCCCGCT 571 649 3314 Antisense
Sequence Source Affymetrix Proprietary Database