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[181798_at]

Description C25H3.8 /REP_DB=WormBase Gene ID /WP=CE02506 /TR=Q18183 /GB=AAK31453.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 24      4grp_high_8_8 > 25      6grp_highest_nonorm > 61      6grp_total > 25      4grp_total > 47      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 558
1 2 3 4 range
A 664.2
(3)
P 		355.8
(-1.1)
P 		216.9
(-3)
P 		351.7
(-1.2)
P 		447
B 767.4
(3)
P 		244.2
(-2.6)
P 		209.5
(-3)
P 		277
(-2.3)
P 		558
C 581.9
(3)
P 		222.2
(-3)
P 		277.2
(-2.1)
P 		278.3
(-2.1)
A 		360
avr 671.2
(3) 		274.1
(-2.5) 		234.5
(-3) 		302.3
(-2.1) 		437

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 441
1 2 3 4 5 6 range
A 532.3
(0.9)
P 		683.7
(3)
P 		242.8
(-3)
P 		431.7
(-0.4)
P 		299.6
(-2.2)
P 		301.8
(-2.2)
P 		441
B 484.4
(1.5)
P 		558.7
(3)
P 		393.3
(-0.2)
P 		307.9
(-1.9)
P 		252
(-3)
P 		334.3
(-1.4)
P 		307
C 599.3
(1.6)
P 		679.5
(3)
P 		597
(1.6)
P 		488.1
(-0.3)
P 		330.9
(-3)
P 		383.5
(-2.1)
P 		349
avr 538.7
(1.2) 		640.6
(3) 		411
(-1) 		409.2
(-1) 		294.2
(-3) 		339.9
(-2.2) 		346
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 198
1 2 3 4 range
A 241.8
(3)
P 		77.2
(-2.2)
P 		56.7
(-2.8)
P 		51.5
(-3)
P 		190
B 244.7
(3)
P 		60.3
(-2.6)
P 		48.6
(-3)
P 		59
(-2.7)
P 		196
C 192.6
(3)
P 		63.9
(-2.3)
P 		96.6
(-1)
P 		46.9
(-3)
A 		146
avr 226.4
(3) 		67.1
(-2.5) 		67.3
(-2.5) 		52.5
(-3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 190
1 2 3 4 5 6 range
A 98.1
(-1.6)
P 		243.2
(3)
P 		52.9
(-3)
P 		109.5
(-1.2)
P 		90.4
(-1.8)
P 		73.5
(-2.4)
P 		190
B 168.5
(2)
P 		186
(3)
P 		95.5
(-2.2)
P 		81.5
(-3)
P 		102.4
(-1.8)
P 		124.7
(-0.5)
P 		105
C 222.1
(3)
P 		207.3
(2.3)
P 		151.9
(-0.4)
P 		98.2
(-3)
P 		107.1
(-2.6)
P 		128.4
(-1.5)
P 		124
avr 162.9
(0.4) 		212.2
(3) 		100.1
(-2.8) 		96.4
(-3) 		100
(-2.8) 		108.9
(-2.4) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181798_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5958
Sequence Type Exemplar sequence
Representative Public ID C25H3.8 NCBI
Target Description C25H3.8 /REP_DB=WormBase Gene ID /WP=CE02506 /TR=Q18183 /GB=AAK31453.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062709(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062709 NCBI Caenorhabditis elegans C25H3.8 (C25H3.8) mRNA, complete cds. 11/11 None
SNAP00000016805 ENSEMBL cdna:SNAP chromosome:CEL140:II:5670239:5680114:-1 10/11 None
GENEFINDER00000016818 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5669089:5679244:-1 11/11 None
C25H3.8 ENSEMBL cdna:known chromosome:CEL140:II:5669745:5678209:-1 gene:C25H3.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5670235-5678206(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25H3.8
Entrez Gene 173962 Entrez gene
SwissProt Q18183 EMBL-EBI
WormBase CE32142 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.12200.1.S1_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.26156.1.S1_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.26156.3.S1_S_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.2669.1.S1_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.2741.1.S1_AT similar to vacuolar protein sorting 13D gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4420 hydroxymethylglutaryl-CoA reductase (NADPH) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31453 Hypothetical protein C25H3.8 [Caenorhabditis elegans] ref|NP_495110.2| C25H3.8 [Caenorhabditis elegans] 0.0
blast T15626 hypothetical protein C25H3.8 - Caenorhabditis elegans 0.0
blast CAE59168 Hypothetical protein CBG02475 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.0E-126
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.0E-126
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000016818 1 2313-2335
Sequence
Target Sequence 
>C. ELEGANS:181798_AT
tggtgtgctcggcggagcaactgccatgttcacgaatgttgcatcggaaagtcgaaagag
tggattagtgaaaggaatggtttggggagttgcgactggtgttgttgatactgtcacaaa
accagttcaaggagtattcgattttgttgaaggaactgcatcggcaatgaaggaattagc
aatgccagctacaggtgtcagaagagcaaccgcactatgccgtgtacgaatccctcgtct
ctgtcgaaacctgtaccacctgctccctgcatacaatccaaacctagctcatgctcagat
ggagcttctcagaatcaatggatactcgtcaagagagcaacttctcgatgttgaaacatg
ccttgagcacatcgacgcggcgaatatagatcgtatcattcgtcaatatgtacttatttc
gacaaaacagtgttatgtttgtcggcagacgaatggagaaagttcgattgtgatccaaag
aatcgcatataaatatttgaagagtgttgttgcgagacctccattggaaaacacgttttc
tagtattgaagtaagtctcgatatggaagatgcacgtctccgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTGTGCTCGGCGGAGCAACTGCC 458 553 5694 Antisense
GAGCAACTGCCATGTTCACGAATGT 184 391 5708 Antisense
GGAATGGTTTGGGGAGTTGCGACTG 155 531 5767 Antisense
GTTGCGACTGGTGTTGTTGATACTG 283 439 5782 Antisense
GCAATGCCAGCTACAGGTGTCAGAA 530 321 5872 Antisense
CAACCGCACTATGCCGTGTACGAAT 94 187 5900 Antisense
TACAATCCAAACCTAGCTCATGCTC 304 641 5965 Antisense
AAACATGCCTTGAGCACATCGACGC 583 127 6047 Antisense
CAGTGTTATGTTTGTCGGCAGACGA 347 205 6121 Antisense
AGAGTGTTGTTGCGAGACCTCCATT 56 69 6194 Antisense
ATATGGAAGATGCACGTCTCCGCCA 251 21 6254 Antisense
Sequence Source Affymetrix Proprietary Database