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[181784_at]

Description F35D2.4 /REP_DB=WormBase Gene ID /WP=CE02726 /TR=Q20046 /GB=AAA68328.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 14      6grp_total > 14      4grp_total > 38      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 127.5
(3)
P 		61.1
(-2.2)
A 		62.8
(-2)
A 		50.4
(-3)
A 		77
B 155.4
(3)
P 		72.7
(-1.6)
A 		46.5
(-3)
A 		56.8
(-2.4)
A 		109
C 153.7
(3)
P 		92.8
(-0.3)
A 		41.4
(-3)
A 		136.1
(2.1)
P 		112
avr 145.5
(3) 		75.5
(-1.4) 		50.2
(-3) 		81.1
(-1.1) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 165.4
(3)
P 		105.2
(-0.2)
P 		74.6
(-1.8)
P 		62.5
(-2.4)
A 		66
(-2.3)
P 		51.8
(-3)
A 		114
B 88.7
(1.6)
P 		94.3
(2.9)
P 		90.1
(1.9)
A 		94.7
(3)
P 		78.3
(-0.8)
A 		68.8
(-3)
P 		26
C 105.8
(2.4)
P 		111.9
(3)
P 		87.6
(0.5)
A 		73.7
(-1)
A 		60
(-2.4)
A 		54.1
(-3)
P 		58
avr 120
(3) 		103.8
(1.4) 		84.1
(-0.5) 		77
(-1.2) 		68.1
(-2) 		58.2
(-3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 46.4
(3)
P 		13.3
(-2.1)
A 		16.4
(-1.6)
A 		7.4
(-3)
A 		39
B 49.6
(3)
P 		18
(-1.9)
A 		10.8
(-3)
A 		12.1
(-2.8)
A 		39
C 50.9
(3)
P 		26.7
(-1)
A 		14.4
(-3)
A 		22.9
(-1.6)
P 		37
avr 49
(3) 		19.3
(-2.1) 		13.9
(-3) 		14.1
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 30.5
(1.3)
P 		37.4
(3)
P 		16.3
(-2.1)
P 		15.8
(-2.2)
A 		19.9
(-1.2)
P 		12.6
(-3)
A 		25
B 30.9
(2.5)
P 		31.4
(2.8)
P 		21.9
(-3)
A 		25.1
(-1.1)
P 		31.8
(3)
A 		25.7
(-0.7)
P 		10
C 39.2
(3)
P 		34.1
(1.7)
P 		22.3
(-1.2)
A 		14.8
(-3)
A 		19.4
(-1.9)
A 		18.1
(-2.2)
P 		24
avr 33.5
(2.7) 		34.3
(3) 		20.2
(-2.4) 		18.6
(-3) 		23.7
(-1.1) 		18.8
(-2.9) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181784_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5581
Sequence Type Exemplar sequence
Representative Public ID F35D2.4 NCBI
Target Description F35D2.4 /REP_DB=WormBase Gene ID /WP=CE02726 /TR=Q20046 /GB=AAA68328.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063190(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063190 NCBI Caenorhabditis elegans F35D2.4 (F35D2.4) mRNA, complete cds. 11/11 None
SNAP00000006816 ENSEMBL cdna:SNAP chromosome:CEL140:II:7580192:7583309:-1 11/11 None
GENEFINDER00000006822 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7580226:7583563:-1 11/11 None
F35D2.4 ENSEMBL cdna:known chromosome:CEL140:II:7580466:7583479:-1 gene:F35D2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7580462-7583476(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35D2.4
Entrez Gene 185278 Entrez gene
SwissProt Q20046 EMBL-EBI
WormBase CE02726 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68328 Hypothetical protein F35D2.4 [Caenorhabditis elegans] ref|NP_495591.1| F35D2.4 [Caenorhabditis elegans] 0.0
blast CAE67494 Hypothetical protein CBG13002 [Caenorhabditis briggsae] 0.0
blast XP_418334 PREDICTED: similar to hypothetical protein MGC26979 [Gallus gallus] 1.0E-73
blast XP_418334 PREDICTED: similar to hypothetical protein MGC26979 [Gallus gallus] 2.0E-64
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495591.1 3 421-443,514-531,559-581
F35D2.4 3 421-443,514-531,559-581
SNAP00000006816 4 391-413,484-501,529-551,746-768
GENEFINDER00000006822 4 449-471,542-559,587-609,774-796
Sequence
Target Sequence 
>C. ELEGANS:181784_AT
gagagagatgccttcgactcttcaacaactctgagcactcttgccagctcattagcggtt
acaatttttgtctatctctcattggcattgctgcaagttcttgtccgagttttgatcgtt
gaacgaatcgtgaccgacccatttcataattttgttgatctttgctctttgtcaaacata
agtgtcctttctctgacccattctctgtatggatactatattcatggtcgttctgttcat
ggaaagggtgatgctggaatgagtgagatgaatgaattcttacaacgcgagcggaataat
ttatgcggattccgaggacttgaaccaggatcagagcttcagactttcacggtaaatcta
ccgaatttgtttcgttcgaaatatgacgaaatttttgcggtctccaaacaaactacatct
ggtgtagttggacatgaagcgatcacggcaaaaatgaatgcaacagtcgatgctcattcc
caaatgaactcatttttaaagaaatttgtagatcattcaataacagatatcgaatatgtt
gttcgggatcgaccagtcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGAGATGCCTTCGACTCTTCAA 398 395 1636 Antisense
GACTCTTCAACAACTCTGAGCACTC 642 373 1651 Antisense
ACTCTTGCCAGCTCATTAGCGGTTA 268 101 1672 Antisense
TCTATCTCTCATTGGCATTGCTGCA 311 615 1706 Antisense
TGAACGAATCGTGACCGACCCATTT 544 571 1755 Antisense
ATTTTGTTGATCTTTGCTCTTTGTC 105 15 1784 Antisense
AACATAAGTGTCCTTTCTCTGACCC 128 139 1810 Antisense
TTCTCTGACCCATTCTCTGTATGGA 189 693 1824 Antisense
GGATCAGAGCTTCAGACTTTCACGG 505 511 1963 Antisense
TGCAACAGTCGATGCTCATTCCCAA 463 577 2094 Antisense
TGTTGTTCGGGATCGACCAGTCTTG 689 561 2172 Antisense
Sequence Source Affymetrix Proprietary Database