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[181780_at]

Description C05H8.2 /REP_DB=WormBase Gene ID /WP=CE01147 /TR=Q17681 /GB=AAA19243.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 21      6grp_cgc > 44      6grp_cgc_sub > 02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 40.5
(0.3)
A 		56.9
(1.8)
A 		4.2
(-3)
A 		69.5
(3)
A 		65
B 62.3
(2.5)
A 		24.5
(-1.2)
A 		67.4
(3)
A 		5.8
(-3)
A 		62
C 44.1
(-0.6)
A 		36.2
(-1.1)
A 		6.5
(-3)
A 		101.2
(3)
A 		95
avr 49
(1.2) 		39.2
(-0.6) 		26
(-3) 		58.8
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 32.7
(-1.4)
A 		49.5
(1)
A 		63.7
(3)
A 		21.9
(-3)
A 		47.9
(0.7)
A 		37
(-0.8)
A 		42
B 55.2
(0.7)
A 		75.3
(3)
A 		54.2
(0.6)
A 		22.9
(-3)
A 		41.6
(-0.9)
A 		28.2
(-2.4)
A 		52
C 78
(3)
A 		63.1
(1)
A 		46.9
(-1.1)
A 		35.5
(-2.6)
A 		32.6
(-3)
A 		44.7
(-1.4)
A 		45
avr 55.3
(1.8) 		62.6
(3) 		54.9
(1.7) 		26.8
(-3) 		40.7
(-0.7) 		36.6
(-1.4) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 14.7
(3)
A 		12.4
(2)
A 		1.1
(-3)
A 		10.2
(1)
A 		14
B 19.9
(3)
A 		6
(-1.5)
A 		15.6
(1.6)
A 		1.2
(-3)
A 		19
C 14.6
(2)
A 		10.4
(0.3)
A 		2.3
(-3)
A 		17.1
(3)
A 		15
avr 16.4
(3) 		9.6
(-1) 		6.3
(-3) 		9.5
(-1.1) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 6
(-2.8)
A 		17.6
(3)
A 		13.9
(1.2)
A 		5.5
(-3)
A 		14.5
(1.5)
A 		9
(-1.3)
A 		12
B 19.2
(1.1)
A 		25.1
(3)
A 		13.2
(-0.8)
A 		6.1
(-3)
A 		16.9
(0.4)
A 		10.5
(-1.6)
A 		19
C 28.9
(3)
A 		19.3
(0.4)
A 		11.9
(-1.7)
A 		7.1
(-3)
A 		10.6
(-2)
A 		15
(-0.8)
A 		22
avr 18
(1.9) 		20.7
(3) 		13
(-0.2) 		6.2
(-3) 		14
(0.2) 		11.5
(-0.8) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181780_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6827
Sequence Type Exemplar sequence
Representative Public ID C05H8.2 NCBI
Target Description C05H8.2 /REP_DB=WormBase Gene ID /WP=CE01147 /TR=Q17681 /GB=AAA19243.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025982
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000011181 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5645053:5650079:1 None
C05H8.1b ENSEMBL cdna:known chromosome:CEL140:III:5635429:5654485:1 gene:C05H8.1 A 
NM_001025982 NCBI Caenorhabditis elegans CaM Kinase Kinase family member (ckk-1) (ckk-1) mRNA, complete cds. None
SNAP00000011176 ENSEMBL cdna:SNAP chromosome:CEL140:III:5645053:5645268:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5645051-5646232(+) 100.0 100.0
Public Domain and Genome References
Ensembl C05H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021153 CaM Kinase Kinase family member (ckk-1) [Caenorhabditis elegans] 0.0
blast AAZ82849 Cam kinase kinase protein 1, isoform b [Caenorhabditis elegans] 0.0
blast AAA19242 Cam kinase kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001021152.1| CaM Kinase Kinase family member (ckk-1) [Caenorhabditis elegans] dbj|BAA77824.4| Ca2+/calmodulin-dependent protein kinase kinase [Caenorhabditis elegans] 0.0
blast AAZ82849 Cam kinase kinase protein 1, isoform b [Caenorhabditis elegans] 4.0E-26
blast NP_001021153 CaM Kinase Kinase family member (ckk-1) [Caenorhabditis elegans] 4.0E-26
blast AAZ82849 Cam kinase kinase protein 1, isoform b [Caenorhabditis elegans] 2.0E-16
blast NP_001021153 CaM Kinase Kinase family member (ckk-1) [Caenorhabditis elegans] 2.0E-16
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-60
hanks 2.2.12 CaMK Group; CaMK II KIN1/SNF1/Nim1; YCL24 2.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 5.2E-73
Sequence
Target Sequence 
>C. ELEGANS:181780_AT
gacacgtcagatgattcgccgggtccctcctcaacagttagcacaatgacaacaactgga
actgttgacaggcggtcactactgtcagcagcttcaatggttcgacaacaatcagataca
acaggtgtacggaggcttgtgagagctcgagccgtgcaagaagatgatgaagccggacct
cattctagtaataatcatatttcaccaagcagccgacacttcatgagtgggtgcacagag
aaactatctaggccctcagcagccgacgcctccccggtcccacgcgtgtcaaatttttca
atgttaagtactcaagtagcgtttttgggcttttgggcttcgtgcttattgtggattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACGTCAGATGATTCGCCGGGTC 488 367 22 Antisense
TCCCTCCTCAACAGTTAGCACAATG 313 597 45 Antisense
GGAACTGTTGACAGGCGGTCACTAC 627 525 79 Antisense
GTCACTACTGTCAGCAGCTTCAATG 499 465 96 Antisense
TTGTGAGAGCTCGAGCCGTGCAAGA 173 707 158 Antisense
TGAAGCCGGACCTCATTCTAGTAAT 478 573 189 Antisense
ATCATATTTCACCAAGCAGCCGACA 213 31 215 Antisense
GCAGCCGACACTTCATGAGTGGGTG 415 313 230 Antisense
AGAGAAACTATCTAGGCCCTCAGCA 362 69 258 Antisense
TCCCACGCGTGTCAAATTTTTCAAT 279 597 299 Antisense
TGGGCTTCGTGCTTATTGTGGATTC 289 555 355 Antisense
Sequence Source Affymetrix Proprietary Database