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[181776_s_at]

Description T08H4.1 /REP_DB=WormBase Gene ID /WP=CE04926 /TR=Q22354 /GB=AAC71118.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 29      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_total > 28      4grp_total > 46      6grp_cgc > 03      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 210
1 2 3 4 range
A 336.8
(3)
P 		239.3
(-0.4)
P 		237.3
(-0.4)
P 		163.4
(-3)
P 		173
B 320.2
(3)
P 		195.9
(-1.6)
P 		191
(-1.8)
P 		158.6
(-3)
P 		162
C 357.3
(3)
P 		245.1
(-0.2)
P 		147.5
(-3)
P 		163.6
(-2.5)
P 		210
avr 338.1
(3) 		226.8
(-0.8) 		191.9
(-2) 		161.9
(-3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 352
1 2 3 4 5 6 range
A 270.6
(0.8)
P 		367.5
(3)
P 		97.4
(-3)
P 		117.9
(-2.5)
P 		221.1
(-0.3)
P 		256.1
(0.5)
P 		270
B 325.9
(3)
P 		319.6
(2.8)
P 		122.1
(-3)
M 		206.5
(-0.5)
P 		186.5
(-1.1)
P 		147.3
(-2.3)
P 		204
C 449.4
(3)
P 		239.6
(-1)
P 		136.6
(-3)
P 		156.5
(-2.6)
P 		251.7
(-0.8)
P 		184.1
(-2.1)
P 		313
avr 348.6
(3) 		308.9
(2) 		118.7
(-3) 		160.3
(-1.9) 		219.8
(-0.4) 		195.8
(-1) 		230
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 122.6
(3)
P 		51.9
(-1.3)
P 		62
(-0.7)
P 		23.9
(-3)
P 		99
B 102.1
(3)
P 		48.4
(-1.7)
P 		44.3
(-2.1)
P 		33.8
(-3)
P 		68
C 118.3
(3)
P 		70.5
(-0.2)
P 		51.4
(-1.4)
P 		27.6
(-3)
P 		91
avr 114.3
(3) 		56.9
(-1) 		52.6
(-1.3) 		28.4
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 49.9
(-1.4)
P 		130.7
(3)
P 		21.2
(-3)
P 		29.9
(-2.5)
P 		66.7
(-0.5)
P 		62.4
(-0.7)
P 		110
B 113.4
(3)
P 		106.4
(2.5)
P 		29.7
(-3)
M 		54.7
(-1.2)
P 		75.8
(0.3)
P 		54.9
(-1.2)
P 		84
C 166.6
(3)
P 		73.1
(-1.2)
P 		34.8
(-2.9)
P 		31.5
(-3)
P 		81.5
(-0.8)
P 		61.6
(-1.7)
P 		135
avr 110
(3) 		103.4
(2.5) 		28.6
(-3) 		38.7
(-2.3) 		74.7
(0.4) 		59.6
(-0.7) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181776_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5470
Sequence Type Exemplar sequence
Representative Public ID T08H4.1 NCBI
Target Description T08H4.1 /REP_DB=WormBase Gene ID /WP=CE04926 /TR=Q22354 /GB=AAC71118.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062322(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062322 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-127) (tag-127) mRNA, complete cds. 11/11 None
SNAP00000025211 ENSEMBL cdna:SNAP chromosome:CEL140:II:4120298:4125441:1 11/11 None
GENEFINDER00000025218 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4116086:4126213:1 11/11 None
T08H4.1 ENSEMBL cdna:known chromosome:CEL140:II:4120331:4127843:1 gene:T08H4.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4120329-4125436(+) 99.61 99.61
Public Domain and Genome References
Gene Title
Gene Symbol tag-127
Ensembl T08H4.1
Entrez Gene 173748 Entrez gene
SwissProt Q22354 EMBL-EBI
WormBase CE04926 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71118 Temporarily assigned gene name protein 127 [Caenorhabditis elegans] ref|NP_494723.1| Temporarily Assigned Gene name family member (tag-127) [Caenorhabditis elegans] 0.0
blast CAE71513 Hypothetical protein CBG18446 [Caenorhabditis briggsae] 0.0
blast XP_536728 PREDICTED: similar to neuronal RhoA GEF protein [Canis familiaris] 1.0E-52
blast NP_001004156 neuronal RhoA GEF protein [Mus musculus] gb|AAU04954.1| RhoA-specific guanine exchange factor SYX2 [Mus musculus] 1.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR000219 EMBL-EBI DH domain 3.8E-7
Pfam IPR000219 EMBL-EBI DH domain 3.8E-7
Pfam IPR000219 EMBL-EBI DH domain 3.8E-7
Sequence
Target Sequence 
>C. ELEGANS:181776_S_AT
atatcaaagctgcctggagccatcctaaaacgattctcattgaacggaggttcaggggcc
gtaggaagcagttgcaagaaactggagagctcgaaaaggaactcacaggcttcgaatgct
gctagtttggactctttcggaagttccacaagcgggatttccacggcctcgtcgaataac
aacgatcccagcatggaaacgttaacgtcaaaactctcccacatttctaccatttcaatc
aacgactcgccatcttccattgactctcagggcacgctgagcatcagcttggaagctcca
cctacagttttagagaaagatgaagatcaacactcgatcgaagtaagtgtcccaccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATCAAAGCTGCCTGGAGCCATCC 707 17 2110 Antisense
GGAGCCATCCTAAAACGATTCTCAT 79 523 2125 Antisense
GAGGTTCAGGGGCCGTAGGAAGCAG 364 399 2156 Antisense
GAACTCACAGGCTTCGAATGCTGCT 21 351 2208 Antisense
GAATGCTGCTAGTTTGGACTCTTTC 501 331 2223 Antisense
GAAGTTCCACAAGCGGGATTTCCAC 441 335 2249 Antisense
TTTCCACGGCCTCGTCGAATAACAA 219 661 2267 Antisense
TACCATTTCAATCAACGACTCGCCA 407 649 2337 Antisense
GACTCTCAGGGCACGCTGAGCATCA 76 375 2371 Antisense
GGAAGCTCCACCTACAGTTTTAGAG 689 529 2400 Antisense
TCGATCGAAGTAAGTGTCCCACCGC 708 601 2443 Antisense
Sequence Source Affymetrix Proprietary Database