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[181775_at]

Description C14A4.14 /REP_DB=WormBase Gene ID /WP=CE24801 /TR=Q9U3Q0 /GB=CAB60995.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 62      6grp_total > 50      4grp_total > 56      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 819
1 2 3 4 range
A 565
(-0.8)
P 		705.5
(0.6)
P 		936
(3)
P 		357.4
(-3)
P 		579
B 475.6
(-1.2)
P 		638
(0.7)
P 		825.8
(3)
P 		329.2
(-3)
P 		497
C 678.1
(0.2)
P 		655.2
(-0)
P 		1066.8
(3)
P 		248
(-3)
P 		819
avr 572.9
(-0.5) 		666.2
(0.4) 		942.9
(3) 		311.5
(-3) 		631

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 419
1 2 3 4 5 6 range
A 415.2
(-1.1)
P 		458.8
(0.2)
P 		550.3
(3)
P 		433.6
(-0.5)
P 		435.8
(-0.5)
P 		353
(-3)
P 		197
B 469.1
(-0.8)
P 		436.2
(-1.4)
P 		683.2
(3)
P 		567.7
(0.9)
P 		347.6
(-3)
P 		596.8
(1.5)
P 		336
C 586.5
(2.4)
P 		621.8
(3)
P 		391.6
(-0.9)
P 		595.1
(2.6)
P 		264.2
(-3)
P 		496.1
(0.9)
P 		358
avr 490.3
(1.4) 		505.6
(1.9) 		541.7
(3) 		532.1
(2.7) 		349.2
(-3) 		482
(1.1) 		193
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 330
1 2 3 4 range
A 205.7
(1.8)
P 		153.2
(0.1)
P 		244.7
(3)
P 		52.3
(-3)
P 		192
B 151.7
(1)
P 		157.6
(1.3)
P 		191.4
(3)
P 		70.1
(-3)
P 		121
C 224.5
(0.3)
P 		188.4
(-0.3)
P 		371.8
(3)
P 		41.8
(-3)
P 		330
avr 194
(0.9) 		166.4
(0.1) 		269.3
(3) 		54.7
(-3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 76.5
(-3)
P 		163.2
(3)
P 		119.9
(0)
P 		110
(-0.7)
P 		131.5
(0.8)
P 		85.9
(-2.3)
P 		87
B 163.2
(-1.4)
P 		145.2
(-2.7)
P 		165.9
(-1.2)
P 		150.4
(-2.3)
P 		141.2
(-3)
P 		222.6
(3)
P 		81
C 217.4
(3)
P 		189.7
(1.7)
P 		99.7
(-2.4)
P 		119.7
(-1.4)
P 		85.5
(-3)
P 		166.1
(0.7)
P 		132
avr 152.4
(1.2) 		166
(3) 		128.5
(-1.8) 		126.7
(-2.1) 		119.4
(-3) 		158.2
(2) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181775_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4021
Sequence Type Exemplar sequence
Representative Public ID C14A4.14 NCBI
Target Description C14A4.14 /REP_DB=WormBase Gene ID /WP=CE24801 /TR=Q9U3Q0 /GB=CAB60995.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063880(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063880 NCBI Caenorhabditis elegans C14A4.14 (C14A4.14) mRNA, complete cds. 11/11 None
SNAP00000002484 ENSEMBL cdna:SNAP chromosome:CEL140:II:10582541:10583987:1 11/11 None
GENEFINDER00000002508 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10581045:10583987:1 11/11 None
C14A4.14 ENSEMBL cdna:known chromosome:CEL140:II:10582529:10584129:1 gene:C14A4.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10582537-10583984(+) 92.44 100.0
Public Domain and Genome References
Gene Title
Ensembl C14A4.14
Entrez Gene 174632 Entrez gene
SwissProt Q9U3Q0 EMBL-EBI
WormBase CE24801 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.12259.1.S1_AT similar to Mitochondrial 28S ribosomal protein S22 (S22mt) (MRP-S22) cfa
DROSOPHILA_2:1628169_AT mitochondrial ribosomal protein S22 dm
DROSGENOME1:152141_AT mitochondrial ribosomal protein S22 dm
Protein Similarities
Method ID Description E-Value
blast CAB60995 Hypothetical protein C14A4.14 [Caenorhabditis elegans] ref|NP_496281.1| C14A4.14 [Caenorhabditis elegans] 0.0
blast T19244 hypothetical protein C14A4.2 - Caenorhabditis elegans 0.0
blast CAB60994 Hypothetical protein C14A4.2 [Caenorhabditis elegans] ref|NP_496280.1| mammalian cell Death Associated Protein related family member (dap-3) [Caenorhabditis elegans] gb|AAD20727.1| unknown [Caenorhabditis elegans] 0.0
blast EAL27049 GA11515-PA [Drosophila pseudoobscura] 9.0E-37
Sequence
Target Sequence 
>C. ELEGANS:181775_AT
ccggcctatgttcgtttgagtcaactcgtatttgatcgtatcaatgaatctggtaaattc
catgtcgtaacctcaactcgtcactttggaccattcactttttatttggcactgaacgat
aacattcagaagcttttgaactattttggtggacttggaagattatccgactgtgcaaat
cttgtgagacttcagaaggctgttcgtcccgattggagagttacaattgctcagggagat
tctgatgagaagattgtaaaggattttgcgaagcagaatgctcgattccgagatgaaatt
caggatcttttgaattttatcaacaatggaaaattgaaatcagatcaggagcagacggag
cacgtgagggaacgatttggagtacgagtggacaagagaaaagcacgcatcacttcagcc
aatattcgtggagccgatggaccacttggtgattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGGCCTATGTTCGTTTGAGTCAAC 350 265 652 Antisense
GTAAATTCCATGTCGTAACCTCAAC 220 461 704 Antisense
TAACCTCAACTCGTCACTTTGGACC 33 639 719 Antisense
CACTTTGGACCATTCACTTTTTATT 598 197 733 Antisense
TTATCCGACTGTGCAAATCTTGTGA 120 681 814 Antisense
GCTGTTCGTCCCGATTGGAGAGTTA 466 295 850 Antisense
GAAGCAGAATGCTCGATTCCGAGAT 457 341 921 Antisense
AGATCAGGAGCAGACGGAGCACGTG 210 67 993 Antisense
GAGAAAAGCACGCATCACTTCAGCC 219 393 1047 Antisense
ACTTCAGCCAATATTCGTGGAGCCG 322 101 1063 Antisense
GAGCCGATGGACCACTTGGTGATTT 393 383 1082 Antisense
Sequence Source Affymetrix Proprietary Database