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[181749_at]

Description T21B10.3 /REP_DB=WormBase Gene ID /WP=CE23993 /TR=Q22624 /GB=CAA92693.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 85      6grp_total > 56      4grp_total > 62      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 412
1 2 3 4 range
A 878.9
(3)
P 		659.9
(-0.3)
P 		697.9
(0.2)
P 		484.3
(-3)
P 		395
B 870.5
(3)
P 		768.7
(0.8)
P 		751
(0.4)
P 		591.4
(-3)
P 		279
C 895.8
(3)
P 		774.6
(0.9)
P 		803.4
(1.4)
P 		555.7
(-3)
P 		340
avr 881.7
(3) 		734.4
(0.4) 		750.8
(0.7) 		543.8
(-3) 		338

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 674.7
(-2.6)
P 		904.9
(1.9)
P 		653.7
(-3)
P 		655
(-3)
P 		685.6
(-2.4)
P 		958.9
(3)
P 		305
B 919
(0)
P 		927.5
(0.2)
P 		869.4
(-0.6)
P 		772.7
(-1.9)
P 		692.9
(-3)
P 		1139
(3)
P 		446
C 799.6
(-0.8)
P 		903.7
(0.6)
P 		808.9
(-0.7)
P 		822.9
(-0.5)
P 		633.7
(-3)
P 		1082
(3)
P 		448
avr 797.8
(-1) 		912
(0.7) 		777.3
(-1.4) 		750.2
(-1.8) 		670.7
(-3) 		1060
(3) 		389
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 249
1 2 3 4 range
A 320
(3)
P 		143.2
(-1.3)
P 		182.5
(-0.3)
P 		70.9
(-3)
P 		249
B 277.6
(3)
P 		189.9
(-0.5)
P 		174
(-1.1)
P 		126
(-3)
P 		152
C 296.5
(3)
P 		222.7
(0.8)
P 		280
(2.5)
P 		93.7
(-3)
P 		203
avr 298
(3) 		185.3
(-0.4) 		212.2
(0.4) 		96.9
(-3) 		201

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 300
1 2 3 4 5 6 range
A 124.4
(-3)
P 		321.8
(3)
P 		142.4
(-2.5)
P 		166.1
(-1.7)
P 		206.9
(-0.5)
P 		233.5
(0.3)
P 		197
B 319.8
(0.1)
P 		308.7
(-0.2)
P 		211.1
(-2.8)
P 		204.7
(-3)
P 		281.5
(-0.9)
P 		424.7
(3)
P 		220
C 296.4
(1)
P 		275.8
(0.4)
P 		205.9
(-1.8)
P 		165.5
(-3)
P 		205.1
(-1.8)
P 		362.2
(3)
P 		197
avr 246.9
(-0.5) 		302.1
(1.6) 		186.5
(-2.7) 		178.8
(-3) 		231.2
(-1.1) 		340.1
(3) 		161
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181749_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3168
Sequence Type Exemplar sequence
Representative Public ID T21B10.3 NCBI
Target Description T21B10.3 /REP_DB=WormBase Gene ID /WP=CE23993 /TR=Q22624 /GB=CAA92693.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063500(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063500 NCBI Caenorhabditis elegans T21B10.3 (T21B10.3) mRNA, complete cds. 11/11 None
SNAP00000044365 ENSEMBL cdna:SNAP chromosome:CEL140:II:8935429:8938773:1 11/11 None
GENEFINDER00000044371 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8934318:8938773:1 11/11 None
T21B10.3 ENSEMBL cdna:known chromosome:CEL140:II:8934292:8938934:1 gene:T21B10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8934314-8938770(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21B10.3
Entrez Gene 174424 Entrez gene
SwissProt Q22624 EMBL-EBI
WormBase CE23993 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92693 Hypothetical protein T21B10.3 [Caenorhabditis elegans] ref|NP_495901.1| T21B10.3 [Caenorhabditis elegans] 0.0
blast CAE59763 Hypothetical protein CBG03215 [Caenorhabditis briggsae] 0.0
blast CAE63712 Hypothetical protein CBG08229 [Caenorhabditis briggsae] 1.0E-33
blast CAE63712 Hypothetical protein CBG08229 [Caenorhabditis briggsae] 3.0E-33
Sequence
Target Sequence 
>C. ELEGANS:181749_AT
cccacgtttttgcacccagagaacgagagaatgtgtaccagaacagcagtcatgatcgtg
atattccacgtaatgattattcagaatatgatcaagaaaagttgtaccttgacgataaac
aacctcgttttcattctgaaaatagaaattccaatgatgttagaagatattctgaatcaa
aagactcgaaatatgacaataaaaattggaataatcgttcttctcggtggccttctgaat
ctgaatattctcaagaagattctgaaaataggcatctcgatcaaactatccaatccgttc
gaaagttctcatatcgagagaaccaacaaaaacataacagacctggcacctctagtgctt
cgccgccgaaaaaccaagaatggaaacaacatcagcaacagaagaaaagtttcggtggtg
gttttggagaaaacggcaataagttcaatggaaactcacggaaacaacccgagagaaaac
caagatacacttcctcgaacttctacgaacaagatgaagattcacttcaactattgaaac
ctgacagaaatgagcttgctcaggcaatgggcctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCACGTTTTTGCACCCAGAGAACG 697 257 2897 Antisense
GTACCAGAACAGCAGTCATGATCGT 550 457 2931 Antisense
TAAACAACCTCGTTTTCATTCTGAA 132 641 3012 Antisense
TGGAATAATCGTTCTTCTCGGTGGC 52 547 3103 Antisense
CTCGGTGGCCTTCTGAATCTGAATA 223 229 3119 Antisense
GGCATCTCGATCAAACTATCCAATC 338 537 3167 Antisense
ATCCAATCCGTTCGAAAGTTCTCAT 98 41 3184 Antisense
TAACAGACCTGGCACCTCTAGTGCT 680 637 3231 Antisense
TGCTTCGCCGCCGAAAAACCAAGAA 675 579 3252 Antisense
AAACCAAGATACACTTCCTCGAACT 244 125 3373 Antisense
GAGCTTGCTCAGGCAATGGGCCTCA 411 387 3448 Antisense
Sequence Source Affymetrix Proprietary Database