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[181748_at]

Description F34D6.4 /REP_DB=WormBase Gene ID /WP=CE09911 /TR=O17186 /GB=AAC71154.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 13      6grp_total > 13      4grp_total > 47      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 304.4
(3)
P 		81.4
(-3)
A 		86.5
(-2.9)
A 		113.9
(-2.1)
P 		223
B 285.3
(3)
P 		117.7
(-2.4)
A 		104.3
(-2.8)
A 		97.8
(-3)
P 		188
C 243.2
(3)
P 		100.2
(-2.1)
P 		76
(-3)
A 		117.6
(-1.5)
P 		167
avr 277.6
(3) 		99.8
(-2.7) 		88.9
(-3) 		109.8
(-2.3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 210.5
(2.3)
P 		229.1
(3)
P 		153.1
(-0)
P 		154.7
(0.1)
A 		115.3
(-1.5)
P 		77.4
(-3)
P 		152
B 285.8
(3)
P 		254.2
(2.1)
P 		152.5
(-0.8)
P 		139
(-1.2)
P 		114.8
(-1.9)
P 		77.8
(-3)
P 		208
C 203.8
(0.8)
P 		288.4
(3)
P 		182.2
(0.2)
P 		155.1
(-0.5)
P 		99
(-1.9)
P 		57.9
(-3)
P 		231
avr 233.4
(2.2) 		257.2
(3) 		162.6
(-0) 		149.6
(-0.5) 		109.7
(-1.8) 		71
(-3) 		186
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 110.8
(3)
P 		17.7
(-2.9)
A 		22.6
(-2.6)
A 		16.7
(-3)
P 		94
B 91
(3)
P 		29.1
(-2.3)
A 		24.2
(-2.7)
A 		20.8
(-3)
P 		70
C 80.5
(3)
P 		28.8
(-2.1)
P 		26.5
(-2.3)
A 		19.8
(-3)
P 		61
avr 94.1
(3) 		25.2
(-2.5) 		24.4
(-2.6) 		19.1
(-3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 38.8
(-1.1)
P 		81.5
(3)
P 		33.3
(-1.6)
P 		39.2
(-1)
A 		34.8
(-1.5)
P 		18.8
(-3)
P 		63
B 99.5
(3)
P 		84.6
(1.7)
P 		37
(-2.3)
P 		36.8
(-2.3)
P 		46.6
(-1.5)
P 		29
(-3)
P 		71
C 75.5
(1.9)
P 		88
(3)
P 		46.4
(-0.6)
P 		31.2
(-2)
P 		32
(-1.9)
P 		19.4
(-3)
P 		69
avr 71.3
(1.7) 		84.7
(3) 		38.9
(-1.4) 		35.7
(-1.7) 		37.8
(-1.5) 		22.4
(-3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181748_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5941
Sequence Type Exemplar sequence
Representative Public ID F34D6.4 NCBI
Target Description F34D6.4 /REP_DB=WormBase Gene ID /WP=CE09911 /TR=O17186 /GB=AAC71154.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061933(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061933 NCBI Caenorhabditis elegans F34D6.4 (F34D6.4) mRNA, complete cds. 11/11 None
GENEFINDER00000012263 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2690516:2696124:-1 9/11 None
F34D6.4 ENSEMBL cdna:known chromosome:CEL140:II:2690472:2694311:-1 gene:F34D6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012254 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2690545-2694310(-) 92.54 99.59
Public Domain and Genome References
Gene Title
Ensembl F34D6.4
Entrez Gene 185242 Entrez gene
SwissProt O17186 EMBL-EBI
WormBase CE36838 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.29640.1.S1_S_AT similar to euchromatic histone methyltransferase 1 cfa
CANINE_2:CFAAFFX.29642.1.S1_S_AT similar to euchromatic histone methyltransferase 1 cfa
HG-U133_PLUS_2:219339_S_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133A:219339_S_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133A_2:219339_S_AT euchromatic histone-lysine N-methyltransferase 1 hs
HU35KSUBB:RC_R38436_AT euchromatic histone-lysine N-methyltransferase 1 hs
HU35KSUBC:RC_AA285145_AT euchromatic histone-lysine N-methyltransferase 1 hs
U133_X3P:G13376092_3P_A_AT euchromatic histone-lysine N-methyltransferase 1 hs
U133_X3P:HS.127236.1.S1_3P_X_AT euchromatic histone-lysine N-methyltransferase 1 hs
U133_X3P:HS.127236.1.S1_3P_AT euchromatic histone-lysine N-methyltransferase 1 hs
U133_X3P:HS.127236.0.S1_3P_A_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133_PLUS_2:222873_S_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133B:222873_S_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133_PLUS_2:225461_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133B:225461_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95B:52640_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95C:55980_I_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95C:55981_F_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95C:64700_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95E:82086_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95E:89907_AT euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95B:42638_AT Euchromatic histone-lysine N-methyltransferase 1 hs
HU35KSUBD:RC_AA609138_AT Euchromatic histone-lysine N-methyltransferase 1 hs
U133_X3P:HS2.304405.1.A1_3P_AT Euchromatic histone-lysine N-methyltransferase 1 hs
HG-U133_PLUS_2:1559311_AT Euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95E:90081_AT Euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95C:56282_AT Euchromatic histone-lysine N-methyltransferase 1 hs
HG-U95D:74564_AT Euchromatic histone-lysine N-methyltransferase 1 hs
HU35KSUBD:RC_T78584_AT Euchromatic histone-lysine N-methyltransferase 1 hs
MG-U74BV2:109410_AT euchromatic histone methyltransferase 1 mm
MOE430B:1454776_AT euchromatic histone methyltransferase 1 mm
MOUSE430_2:1454776_AT euchromatic histone methyltransferase 1 mm
MG-U74CV2:134238_AT Euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA mm
MU19KSUBA:TC21017_AT Euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA mm
MU19KSUBA:TC21102_AT Euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA mm
MU19KSUBA:TC21103_I_AT Euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA mm
MU19KSUBA:TC21103_S_AT Euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, mRNA mm
RG-U34B:RC_AA965108_AT euchromatic histone methyltransferase 1 (predicted) rn
RAE230A:1376578_AT euchromatic histone methyltransferase 1 (predicted) rn
RAT230_2:1376578_AT euchromatic histone methyltransferase 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16568 chromatin modification inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
18024 histone-lysine N-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC71154 Hypothetical protein F34D6.4 [Caenorhabditis elegans] ref|NP_494334.3| F34D6.4 [Caenorhabditis elegans] 0.0
blast AAC71154 Hypothetical protein F34D6.4 [Caenorhabditis elegans] ref|NP_494334.3| F34D6.4 [Caenorhabditis elegans] 1.0E-118
blast CAE72403 Hypothetical protein CBG19562 [Caenorhabditis briggsae] 3.0E-63
blast CAE72403 Hypothetical protein CBG19562 [Caenorhabditis briggsae] 4.0E-63
blast XP_887518 PREDICTED: similar to HLA-B associated transcript 8 isoform a isoform 5 [Bos taurus] 2.0E-33
blast XP_887518 PREDICTED: similar to HLA-B associated transcript 8 isoform a isoform 5 [Bos taurus] 8.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001214 EMBL-EBI Nuclear protein SET 3.1E-46
Pfam IPR001214 EMBL-EBI Nuclear protein SET 3.1E-46
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494334.3 2 37-59,66-88
F34D6.4 2 37-59,66-88
GENEFINDER00000012263 1 24-46
Sequence
Target Sequence 
>C. ELEGANS:181748_AT
caattagcttgccggagtgcttcgccaacttttatgtgccaatgcgctggtcagtgctca
acaaattgtgaatgctcatcaggggttttcggggaaggaggaaccgtagaaaacatggaa
ctcctcatgtgggacacagtccgagaatgcaacgagtactgtaattgtgctctgtggtgt
gggaaccgagtggcgcaaaaaggtgccatgtaccctgttgagattttcgctcgggatcct
tggtgtgggtggggggtccgtgcatccgtcgacattgcatttggcacatttattggagaa
tatgctggtgagctcatcgatgatgaagaagccatggataggcatgattccacgtttttg
ttcgagactaaagtgggctctgaaactctgacaattgacgcaaaatactcgggaaattac
acaagatttatcaaccacagttgtgcgccaaatgttaaggtagccaacataagctgggac
tacgacaagattcagctcatccacatgtgctttttcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATTAGCTTGCCGGAGTGCTTCGC 319 179 595 Antisense
GTGCTTCGCCAACTTTTATGTGCCA 665 479 611 Antisense
CAATGCGCTGGTCAGTGCTCAACAA 142 183 634 Antisense
AATGCTCATCAGGGGTTTTCGGGGA 598 163 665 Antisense
GAACTCCTCATGTGGGACACAGTCC 223 349 712 Antisense
CTGTAATTGTGCTCTGTGGTGTGGG 135 239 753 Antisense
GTGCCATGTACCCTGTTGAGATTTT 109 479 797 Antisense
CGTGCATCCGTCGACATTGCATTTG 416 245 853 Antisense
GGCATGATTCCACGTTTTTGTTCGA 373 537 935 Antisense
GTGGGCTCTGAAACTCTGACAATTG 321 489 967 Antisense
AGCTCATCCACATGTGCTTTTTCAC 487 79 1088 Antisense
Sequence Source Affymetrix Proprietary Database