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[181746_s_at]

Description Y46G5A.1 /REP_DB=WormBase Gene ID /WP=CE24287 /TR=Q9U2D8 /GB=CAB60374.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 08      4grp_highest_8_8 > 0      4grp_high_8_8 > 43      6grp_highest_nonorm > 71      6grp_total > 19      4grp_total > 44      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 342.1
(1.1)
P 		362
(1.7)
P 		405.7
(3)
P 		206.6
(-3)
P 		199
B 370.5
(1.6)
P 		419.7
(3)
P 		207.6
(-3)
P 		364.7
(1.4)
P 		212
C 299.3
(0.7)
P 		362.6
(3)
P 		267.1
(-0.5)
P 		196.8
(-3)
P 		166
avr 337.3
(0.9) 		381.4
(3) 		293.5
(-1.2) 		256
(-3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 454.4
(3)
P 		307.4
(-1)
P 		293.6
(-1.4)
P 		450.3
(2.9)
P 		351
(0.2)
P 		236
(-3)
P 		218
B 510.3
(3)
P 		346.4
(0)
P 		287
(-1)
P 		267.8
(-1.4)
P 		386.7
(0.8)
P 		179.3
(-3)
P 		331
C 497
(3)
P 		340.3
(-0.2)
P 		314.9
(-0.7)
P 		280.9
(-1.4)
P 		326.6
(-0.5)
P 		204.2
(-3)
P 		293
avr 487.2
(3) 		331.4
(-0.3) 		298.5
(-1) 		333
(-0.3) 		354.8
(0.2) 		206.5
(-3) 		281
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 124.5
(3)
P 		78.6
(0.1)
P 		106
(1.8)
P 		30.2
(-3)
P 		94
B 118.1
(3)
P 		103.7
(1.8)
P 		48.1
(-3)
P 		77.7
(-0.5)
P 		70
C 99.1
(2.6)
P 		104.2
(3)
P 		93.1
(2.1)
P 		33.2
(-3)
P 		71
avr 113.9
(3) 		95.5
(1.3) 		82.4
(0.2) 		47
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 83.8
(-0.2)
P 		109.3
(2.5)
P 		63.9
(-2.3)
P 		114.2
(3)
P 		105.9
(2.1)
P 		57.5
(-3)
P 		57
B 177.6
(3)
P 		115.3
(-0.4)
P 		69.7
(-2.8)
P 		70.9
(-2.8)
P 		157.1
(1.9)
P 		66.8
(-3)
P 		111
C 184.2
(3)
P 		103.8
(-0.8)
P 		80.2
(-1.9)
P 		56.5
(-3)
P 		105.7
(-0.7)
P 		68.3
(-2.4)
P 		128
avr 148.5
(3) 		109.5
(0.2) 		71.3
(-2.5) 		80.5
(-1.8) 		122.9
(1.2) 		64.2
(-3) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181746_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3297
Sequence Type Exemplar sequence
Representative Public ID Y46G5A.1 NCBI
Target Description Y46G5A.1 /REP_DB=WormBase Gene ID /WP=CE24287 /TR=Q9U2D8 /GB=CAB60374.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027263(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027263 NCBI Caenorhabditis elegans Y46G5A.1a (Y46G5A.1) mRNA, complete cds. 11/11 None
SNAP00000038986 ENSEMBL cdna:SNAP chromosome:CEL140:II:12715599:12737918:1 9/11 None
GENEFINDER00000039038 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12715599:12725041:1 11/11 None
Y46G5A.1a ENSEMBL cdna:known chromosome:CEL140:II:12694308:12725371:1 gene:Y46G5A.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027264 8/11 Cross Hyb Matching Probes None
Y46G5A.1b.1 8/11 Cross Hyb Matching Probes A 
Y46G5A.1b.2 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174900 2 1 NM_001027263 181746_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12716705-12725038(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y46G5A.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60374 Hypothetical protein Y46G5A.1a [Caenorhabditis elegans] ref|NP_001022434.1| Y46G5A.1a [Caenorhabditis elegans] 0.0
blast CAE45741 Hypothetical protein Y46G5A.1b [Caenorhabditis elegans] ref|NP_001022435.1| Y46G5A.1b [Caenorhabditis elegans] 0.0
blast CAE63457 Hypothetical protein CBG07920 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.1E-27
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.1E-27
Sequence
Target Sequence 
>C. ELEGANS:181746_S_AT
gattcagtcgagacgtagtcgtgcgaattccaccggtcgttcaccatatccgaaacgaaa
agagtcggctcgcggagccggagccccgaaaggaccgccacctgtacaacataaatggaa
tggtaacgcgtcacaggattcgatattgggcaatggtggagggggtggagccgggggacg
ggaagttccatttgagatcccaactcgccgagcacctaacggttccccggcagcagcagc
acctacagacgcctacaacaactccaaaaacggagcccttccgccgtccggttcgagtaa
gcgagactttgttcagtcttccaacactcccaccacaaccacccactcatcaagttccca
tcggtatcagcattcgaattcatctcgtggaggcaccccgctggcacaacgactccgaga
cgaacgcagcggacaagtggtcctactcagccgagccagtgactcggagcccgacgttgg
ctcaccgatgactaatacaagagactccgactggaatggctctgccgcccacatctcgac
ggctacttcgagaatcacgcattctggaaatcacatcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCAGTCGAGACGTAGTCGTGCG 511 431 1155 Antisense
GTAACGCGTCACAGGATTCGATATT 587 461 1277 Antisense
GCCGTCCGGTTCGAGTAAGCGAGAC 274 275 1437 Antisense
GCGAGACTTTGTTCAGTCTTCCAAC 150 291 1455 Antisense
CAAGTTCCCATCGGTATCAGCATTC 675 183 1505 Antisense
TCGAATTCATCTCGTGGAGGCACCC 664 597 1528 Antisense
AACGACTCCGAGACGAACGCAGCGG 342 143 1562 Antisense
GCGGACAAGTGGTCCTACTCAGCCG 635 293 1583 Antisense
CAGCCGAGCCAGTGACTCGGAGCCC 457 201 1602 Antisense
GACGGCTACTTCGAGAATCACGCAT 297 377 1692 Antisense
CACGCATTCTGGAAATCACATCACC 214 201 1710 Antisense
Sequence Source Affymetrix Proprietary Database