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[181742_s_at]

Description ZC328.3 /REP_DB=WormBase Gene ID /WP=CE15185 /TR=O02054 /GB=AAK39377.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 38      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 83      6grp_total > 37      4grp_total > 50      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 389
1 2 3 4 range
A 464.2
(-1.6)
P 		762.3
(3)
P 		598.4
(0.5)
P 		373.7
(-3)
P 		389
B 556.7
(-1.2)
P 		720.1
(2.6)
P 		735.8
(3)
P 		482.7
(-3)
P 		253
C 562.2
(-3)
P 		753.7
(3)
P 		712.3
(1.7)
P 		596.5
(-1.9)
P 		192
avr 527.7
(-2) 		745.4
(3) 		682.2
(1.5) 		484.3
(-3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 457.7
(-0.7)
P 		510.4
(1.8)
P 		408.8
(-3)
P 		423.1
(-2.3)
P 		413.6
(-2.8)
P 		534.7
(3)
P 		126
B 515.4
(3)
P 		421.6
(-2.7)
P 		416.8
(-3)
P 		459.6
(-0.4)
P 		460.6
(-0.3)
P 		478.9
(0.8)
P 		99
C 554.5
(3)
P 		484.8
(-0.4)
P 		489.3
(-0.1)
P 		430
(-3)
P 		451.3
(-2)
P 		430.5
(-3)
P 		125
avr 509.2
(3) 		472.3
(-0.1) 		438.3
(-2.9) 		437.6
(-3) 		441.8
(-2.6) 		481.4
(0.7) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 169
(3)
P 		165.5
(2.8)
P 		156.4
(2.3)
P 		54.7
(-3)
P 		114
B 177.5
(3)
P 		177.8
(3)
P 		170.5
(2.4)
P 		102.9
(-3)
P 		75
C 186.1
(0.5)
P 		216.7
(1.7)
P 		248.3
(3)
P 		100.6
(-3)
P 		148
avr 177.5
(2.2) 		186.7
(2.7) 		191.7
(3) 		86.1
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 84.4
(-3)
P 		181.5
(3)
P 		89
(-2.7)
P 		107.3
(-1.6)
P 		124.8
(-0.5)
P 		130.2
(-0.2)
P 		97
B 179.3
(2.5)
P 		140.3
(-0.3)
P 		101.2
(-3)
P 		121.7
(-1.6)
P 		187.1
(3)
P 		178.6
(2.4)
P 		86
C 205.5
(3)
P 		147.9
(0.1)
P 		124.5
(-1.1)
P 		86.5
(-3)
P 		146
(0)
P 		144.1
(-0.1)
P 		119
avr 156.4
(3) 		156.6
(3) 		104.9
(-3) 		105.2
(-3) 		152.6
(2.5) 		151
(2.4) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181742_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1369
Sequence Type Exemplar sequence
Representative Public ID ZC328.3 NCBI
Target Description ZC328.3 /REP_DB=WormBase Gene ID /WP=CE15185 /TR=O02054 /GB=AAK39377.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059446(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059446 NCBI Caenorhabditis elegans ZC328.3 (ZC328.3) mRNA, complete cds. 11/11 None
SNAP00000023533 ENSEMBL cdna:SNAP chromosome:CEL140:I:6417528:6419532:-1 11/11 None
ZC328.3 ENSEMBL cdna:known chromosome:CEL140:I:6417195:6424999:-1 gene:ZC328.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6417456-6424690(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC328.3
Entrez Gene 172343 Entrez gene
SwissProt O02054 EMBL-EBI
WormBase CE34444 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.16789.1.S1_AT similar to ZC328.3 cfa
CHICKEN:GGA.10038.1.A1_AT similar to DKFZp434C0328 protein gga
HG-U133_PLUS_2:219313_AT hypothetical protein DKFZp434C0328 hs
HG-U133A:219313_AT hypothetical protein DKFZp434C0328 hs
HG-U133A_2:219313_AT hypothetical protein DKFZp434C0328 hs
HU35KSUBB:RC_N29543_AT hypothetical protein DKFZp434C0328 hs
U133_X3P:G8922125_3P_AT hypothetical protein DKFZp434C0328 hs
HG-U95B:43548_AT hypothetical protein DKFZp434C0328 hs
HG-U95C:65369_AT hypothetical protein DKFZp434C0328 hs
HG-U95D:69120_AT hypothetical protein DKFZp434C0328 hs
HG-U95D:72538_AT hypothetical protein DKFZp434C0328 hs
HG-U95D:87452_R_AT Hypothetical protein DKFZp434C0328 hs
HU35KSUBB:RC_AA047164_AT Hypothetical protein DKFZp434C0328 hs
MG-U74BV2:163150_AT RIKEN cDNA 4921521N14 gene mm
MOE430B:1434725_AT RIKEN cDNA 4921521N14 gene mm
MOUSE430_2:1434725_AT RIKEN cDNA 4921521N14 gene mm
Protein Similarities
Method ID Description E-Value
blast AAK39377 Hypothetical protein ZC328.3 [Caenorhabditis elegans] ref|NP_491847.2| ZC328.3 [Caenorhabditis elegans] 0.0
blast CAE67182 Hypothetical protein CBG12618 [Caenorhabditis briggsae] 0.0
blast CAE67182 Hypothetical protein CBG12618 [Caenorhabditis briggsae] 1.0E-107
blast XP_394943 PREDICTED: hypothetical protein XP_394943 [Apis mellifera] 2.0E-50
blast XP_416574 PREDICTED: similar to DKFZp434C0328 protein [Gallus gallus] 3.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR004182 EMBL-EBI GRAM domain 1.8E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491847.2 1 555-577
ZC328.3 1 555-577
SNAP00000023533 1 336-358
Sequence
Target Sequence 
>C. ELEGANS:181742_S_AT
ttcactgtcaattgtcgatactgcatatcaagaactggtccaacatcgtgtagaataaag
gtacacggaggtgttatgtacaagaagagtacatgggcagtggtgaagacattcattgag
aaagggactcatcagggtcttgatgaacattatcaattgttgagtaagcttgttgatgag
tatgcagccaatcatccagacgaggatctcagagccccaatgtcgattacattcccaaat
ggaagagatgtcataaaatcatgcgaatcagagtccacacccaatgaggtccgccaccgt
cgttcacagaagtctccagaacgtcttctggctggtggcgaaatcgcgaaaattgtcgat
gattcgcagtttctccctgaatcacaaatcattcaacctattgcaactaccaataaactg
actgcagttgattacaaaccgttcttgattgctatcattgctcttctgacgttattcttc
at

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCACTGTCAATTGTCGATACTGCA 528 685 1072 Antisense
GAACTGGTCCAACATCGTGTAGAAT 38 347 1103 Antisense
GAAAGGGACTCATCAGGGTCTTGAT 348 361 1191 Antisense
TCCAGACGAGGATCTCAGAGCCCCA 560 589 1266 Antisense
GAGCCCCAATGTCGATTACATTCCC 7 387 1283 Antisense
ATCATGCGAATCAGAGTCCACACCC 425 27 1329 Antisense
TTCACAGAAGTCTCCAGAACGTCTT 501 683 1374 Antisense
CGTCTTCTGGCTGGTGGCGAAATCG 12 247 1393 Antisense
ATGATTCGCAGTTTCTCCCTGAATC 368 47 1430 Antisense
ACAAACCGTTCTTGATTGCTATCAT 139 111 1505 Antisense
TTGCTCTTCTGACGTTATTCTTCAT 600 703 1529 Antisense
Sequence Source Affymetrix Proprietary Database