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[181735_at]

Description ZK546.1 /REP_DB=WormBase Gene ID /WP=CE28524 /TR=Q23529 /GB=AAA68733.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_high_8_8 > 13      6grp_highest_nonorm > 90      6grp_total > 67      4grp_total > 06      6grp_highest_sub > Da      6grp_cgc > 47      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 152.4
(3)
P 		144.9
(2.3)
P 		117.6
(-0.2)
P 		87.5
(-3)
A 		65
B 126.8
(-1.1)
P 		97.7
(-3)
A 		147.3
(0.2)
A 		191.9
(3)
P 		94
C 152.4
(0.2)
P 		110.2
(-3)
M 		139.1
(-0.8)
P 		189.8
(3)
P 		80
avr 143.9
(1.1) 		117.6
(-3) 		134.7
(-0.4) 		156.4
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 95.2
(-3)
P 		189.5
(2.2)
P 		108.4
(-2.3)
P 		132.1
(-1)
P 		140.5
(-0.5)
P 		204.1
(3)
P 		109
B 126.3
(-2.3)
P 		154.1
(-1.3)
P 		268.4
(3)
P 		154.2
(-1.3)
P 		108.4
(-3)
P 		205.2
(0.6)
P 		160
C 239.1
(3)
P 		139.9
(-2.4)
P 		141.8
(-2.3)
P 		129.8
(-3)
P 		194
(0.5)
P 		235.9
(2.8)
P 		109
avr 153.5
(-1.8) 		161.2
(-1.2) 		172.9
(-0.3) 		138.7
(-3) 		147.6
(-2.3) 		215.1
(3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 55.5
(3)
P 		31.5
(-0.4)
P 		30.7
(-0.5)
P 		12.8
(-3)
A 		43
B 40.4
(2.8)
P 		24.1
(-3)
A 		34.1
(0.6)
A 		40.9
(3)
P 		17
C 50.5
(3)
P 		31.7
(-3)
M 		48.5
(2.4)
P 		32
(-2.9)
P 		19
avr 48.8
(3) 		29.1
(-2.9) 		37.8
(-0.3) 		28.6
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 17.6
(-3)
P 		67.4
(3)
P 		23.6
(-2.3)
P 		33.5
(-1.1)
P 		42.4
(-0)
P 		49.7
(0.9)
P 		50
B 43.9
(-2.5)
P 		51.3
(-1.2)
P 		65.2
(1.1)
P 		40.9
(-3)
P 		44
(-2.5)
P 		76.5
(3)
P 		36
C 88.6
(3)
P 		42.7
(-1.4)
P 		36.1
(-2)
P 		26.1
(-3)
P 		62.8
(0.5)
P 		79
(2.1)
P 		63
avr 50
(-0.2) 		53.8
(0.5) 		41.6
(-1.6) 		33.5
(-3) 		49.7
(-0.2) 		68.4
(3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181735_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4199
Sequence Type Exemplar sequence
Representative Public ID ZK546.1 NCBI
Target Description ZK546.1 /REP_DB=WormBase Gene ID /WP=CE28524 /TR=Q23529 /GB=AAA68733.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027351(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027351 NCBI Caenorhabditis elegans ZYGote defective: embryonic lethal family member (zyg-12) (zyg-12) mRNA, complete cds. 11/11 None
GENEFINDER00000028507 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4949896:4952909:1 11/11 None
ZK546.1b ENSEMBL cdna:known chromosome:CEL140:II:4949887:4953477:1 gene:ZK546.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027352 8/11 Cross Hyb Matching Probes None
NM_001027350 8/11 Cross Hyb Matching Probes None
AY487141 7/11 Cross Hyb Matching Probes A 
SNAP00000028503 7/11 Cross Hyb Matching Probes None
ZK546.1a.2 8/11 Cross Hyb Matching Probes A 
ZK546.1c 8/11 Cross Hyb Matching Probes A 
ZK546.1a.1 7/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
3565765 3 1 NM_001027351 181735_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4949893-4952907(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK546.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAS60253 Zygote defective: embryonic lethal protein 12, isoform b [Caenorhabditis elegans] ref|NP_001022522.1| ZYGote defective: embryonic lethal family member (zyg-12) [Caenorhabditis elegans] 0.0
blast Q23529 Zygote defective protein 0.0
blast AAS60254 Zygote defective: embryonic lethal protein 12, isoform c [Caenorhabditis elegans] ref|NP_001022523.1| ZYGote defective: embryonic lethal family member (zyg-12) [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022522.1 1 744-766
ZK546.1b 1 744-766
GENEFINDER00000028507 1 741-763
Sequence
Target Sequence 
>C. ELEGANS:181735_AT
cgctagcggcgacgttgttggtttaaaaattcagctggagaaagcagaagttgaagctca
acagatgagagaagctaaaatgcgtgcggagactaaccaggctcaagttgatgagatact
caaaaaacgaactgctgaattggaggtcaacgcgactgctctgcaaaaagctaaagcagt
gattgatgaactggaatacaattctcgtccagtgagtgaagacagtatgacttctgttca
agctttcaaagaaatgaaagaagaaaatgagaagcttcgccagaaagttgaaaaattgga
gattgagctcaatacagtaacccaaggattcgaacaagagaatcggcttttaacgagtgc
ctcgcatcaacaggttttgaatcgatctatcgacgaagtgatgagcatgagagcacacgc
tggatccgaagagccgcagactctgttggatacacagaaaatgagtggagcacttccatg
gagatcacttgcttccgagacccgtcgagaattgccgacggcgatggctagcattctcgt
ccttggattcctcgtcttcatcgcctggatgttcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCTAGCGGCGACGTTGTTGGTTTA 289 253 1716 Antisense
AATGCGTGCGGAGACTAACCAGGCT 179 167 1794 Antisense
GTCAACGCGACTGCTCTGCAAAAAG 194 465 1861 Antisense
GTATGACTTCTGTTCAAGCTTTCAA 257 455 1940 Antisense
ATCGGCTTTTAACGAGTGCCTCGCA 365 35 2057 Antisense
ATGAGAGCACACGCTGGATCCGAAG 489 45 2122 Antisense
GAGCCGCAGACTCTGTTGGATACAC 69 387 2146 Antisense
CACTTCCATGGAGATCACTTGCTTC 41 197 2186 Antisense
GCTTCCGAGACCCGTCGAGAATTGC 387 305 2206 Antisense
GACGGCGATGGCTAGCATTCTCGTC 687 373 2232 Antisense
TCGTCTTCATCGCCTGGATGTTCAT 178 605 2267 Antisense
Sequence Source Affymetrix Proprietary Database