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[181733_at]

Description C25H3.5 /REP_DB=WormBase Gene ID /WP=CE02503 /TR=Q18184 /GB=AAK31451.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 17      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 19.1
(-3)
A 		83.9
(-0.9)
A 		111.3
(-0)
A 		204.8
(3)
M 		186
B 73.9
(0.6)
A 		72.4
(0.2)
A 		60
(-3)
A 		83.3
(3)
A 		23
C 79.4
(-2.2)
A 		93
(-1.7)
A 		56.1
(-3)
A 		230.6
(3)
P 		175
avr 57.5
(-3) 		83.1
(-1.7) 		75.8
(-2) 		172.9
(3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 71.5
(-0.4)
A 		17.6
(-3)
A 		94.2
(0.8)
A 		139.8
(3)
A 		117.9
(1.9)
A 		77
(-0.1)
A 		122
B 58.6
(-0.9)
A 		97
(1.5)
A 		36
(-2.2)
A 		23.3
(-3)
A 		122.2
(3)
A 		71.5
(-0.1)
A 		99
C 112.2
(1.7)
A 		129
(2.6)
A 		14.2
(-3)
A 		132.7
(2.7)
M 		138.3
(3)
A 		53.6
(-1.1)
A 		124
avr 80.8
(-0.5) 		81.2
(-0.5) 		48.1
(-3) 		98.6
(0.9) 		126.1
(3) 		67.4
(-1.5) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 7
(-3)
A 		18.2
(-0.1)
A 		29.1
(2.8)
A 		30
(3)
M 		23
B 23.6
(3)
A 		17.9
(-0.5)
A 		13.9
(-3)
A 		17.8
(-0.6)
A 		10
C 26.3
(-0.9)
A 		26.7
(-0.8)
A 		19.6
(-3)
A 		38.9
(3)
P 		19
avr 19
(-3) 		20.9
(-1.8) 		20.9
(-1.8) 		28.9
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 13.2
(-1.6)
A 		6.3
(-3)
A 		20.5
(-0.1)
A 		35.5
(3)
A 		35.6
(3)
A 		18.7
(-0.5)
A 		29
B 20.4
(-1)
A 		32.3
(0.6)
A 		8.8
(-2.6)
A 		6.2
(-3)
A 		49.6
(3)
A 		26.7
(-0.2)
A 		43
C 41.6
(2.5)
A 		39.4
(2.2)
A 		3.6
(-3)
A 		26.7
(0.4)
M 		44.7
(3)
A 		18
(-0.9)
A 		41
avr 25.1
(-0.4) 		26
(-0.2) 		11
(-3) 		22.8
(-0.8) 		43.3
(3) 		21.1
(-1.1) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181733_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4194
Sequence Type Exemplar sequence
Representative Public ID C25H3.5 NCBI
Target Description C25H3.5 /REP_DB=WormBase Gene ID /WP=CE02503 /TR=Q18184 /GB=AAK31451.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062710
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062710 NCBI Caenorhabditis elegans C25H3.5 (C25H3.5) mRNA, complete cds. None
C25H3.5 ENSEMBL cdna:known chromosome:CEL140:II:5680963:5682812:1 gene:C25H3.5 None
GENEFINDER00000016826 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5680372:5682394:1 None
SNAP00000016806 ENSEMBL cdna:SNAP chromosome:CEL140:II:5681004:5682394:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5680109-5682202(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25H3.5
Entrez Gene 182924 Entrez gene
SwissProt Q18184 EMBL-EBI
WormBase CE29185 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31451 Hypothetical protein C25H3.5 [Caenorhabditis elegans] ref|NP_495111.1| C25H3.5 [Caenorhabditis elegans] 9.0E-31
blast AAK31451 Hypothetical protein C25H3.5 [Caenorhabditis elegans] ref|NP_495111.1| C25H3.5 [Caenorhabditis elegans] 4.0E-18
blast CAE59169 Hypothetical protein CBG02476 [Caenorhabditis briggsae] 2.0E-17
blast CAE59169 Hypothetical protein CBG02476 [Caenorhabditis briggsae] 1.0E-10
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495111.1 1 7-26
C25H3.5 1 7-26
SNAP00000016806 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:181733_AT
tgcaccatctgaatttccataatattctcgctaataaaaatgatcatggtaatatatcta
catatttacagccaatcgacgaagagcgtccgatcttcatggaacgtcgtgaagcttcag
catttggagatatcattggagagcttaagggaaagggactcggcgggcgaatgaggttgg
taaccatgacaaattcatcatttcctgaacctcaaacctcgaaaatcgaaatctttcccg
cttccgttcactttagaaggatagcgctaaagttcataggttcagtgaagcggatgatta
ggaagtcaaaatttagaaaattagattcccattcaattcttattattgttttgaccaccc
aggaagaatttcgaaaaaaaaacaataaaaaaggaatcggatcctattgtcatgcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCACCATCTGAATTTCCATAATAT 652 577 35 Antisense
TCTACATATTTACAGCCAATCGACG 42 611 91 Antisense
GCCAATCGACGAAGAGCGTCCGATC 604 277 105 Antisense
GCGTCCGATCTTCATGGAACGTCGT 539 291 120 Antisense
GGAACGTCGTGAAGCTTCAGCATTT 64 531 135 Antisense
GGACTCGGCGGGCGAATGAGGTTGG 407 525 190 Antisense
TCATCATTTCCTGAACCTCAAACCT 32 619 229 Antisense
TCGAAAATCGAAATCTTTCCCGCTT 57 601 253 Antisense
TCCCGCTTCCGTTCACTTTAGAAGG 606 595 270 Antisense
TATTATTGTTTTGACCACCCAGGAA 212 663 375 Antisense
GGAATCGGATCCTATTGTCATGCAT 263 533 427 Antisense
Sequence Source Affymetrix Proprietary Database