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[181725_at]

Description K02F6.9 /REP_DB=WormBase Gene ID /WP=CE21017 /TR=O16636 /GB=AAB66107.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 34      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 12.4
(-3)
A 		61.2
(3)
A 		56.5
(2.4)
A 		53.9
(2.1)
A 		49
B 70.4
(3)
A 		12.8
(-2.5)
A 		7.8
(-3)
A 		36.1
(-0.3)
A 		63
C 11.9
(-3)
A 		66.7
(3)
A 		33.7
(-0.6)
A 		49.9
(1.2)
A 		55
avr 31.6
(-3) 		46.9
(3) 		32.7
(-2.6) 		46.6
(2.9) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 37.9
(0.2)
A 		61.1
(3)
A 		21.3
(-1.7)
A 		43.9
(1)
P 		29
(-0.8)
A 		10.6
(-3)
A 		51
B 29.5
(-3)
A 		36.7
(-0.4)
A 		39.9
(0.7)
A 		46.2
(3)
A 		35.7
(-0.8)
P 		33.2
(-1.7)
A 		17
C 35.2
(-3)
P 		65.5
(3)
A 		61.1
(2.1)
A 		59.8
(1.9)
A 		55.1
(0.9)
M 		36.6
(-2.7)
A 		30
avr 34.2
(-1.4) 		54.4
(3) 		40.8
(0) 		50
(2) 		39.9
(-0.2) 		26.8
(-3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 4.5
(-3)
A 		13.3
(2.1)
A 		14.8
(3)
A 		7.9
(-1)
A 		10
B 22.4
(3)
A 		3.2
(-2.6)
A 		1.8
(-3)
A 		7.7
(-1.3)
A 		21
C 3.9
(-3)
A 		19.2
(3)
A 		11.7
(0.1)
A 		8.4
(-1.2)
A 		15
avr 10.3
(0.5) 		11.9
(3) 		9.4
(-0.8) 		8
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 7
(-1.6)
A 		21.7
(3)
A 		4.6
(-2.4)
A 		11.1
(-0.3)
P 		8.7
(-1.1)
A 		2.6
(-3)
A 		19
B 10.3
(-2.3)
A 		12.2
(0.1)
A 		9.7
(-3)
A 		12.2
(0.1)
A 		14.5
(3)
P 		12.4
(0.4)
A 		5
C 13
(-2.3)
P 		20
(3)
A 		15.5
(-0.4)
A 		12
(-3)
A 		17.8
(1.4)
M 		12.2
(-2.9)
A 		8
avr 10.1
(-2.3) 		18
(3) 		9.9
(-2.5) 		11.8
(-1.2) 		13.7
(0.1) 		9.1
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181725_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4071
Sequence Type Exemplar sequence
Representative Public ID K02F6.9 NCBI
Target Description K02F6.9 /REP_DB=WormBase Gene ID /WP=CE21017 /TR=O16636 /GB=AAB66107.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061896(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061896 NCBI Caenorhabditis elegans K02F6.9 (K02F6.9) mRNA, complete cds. 11/11 None
K02F6.9 ENSEMBL cdna:known chromosome:CEL140:II:2557830:2563678:-1 gene:K02F6.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037142 7/11 Cross Hyb Matching Probes None
GENEFINDER00000037146 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2557828-2563677(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K02F6.9
Entrez Gene 186911 Entrez gene
SwissProt O16636 EMBL-EBI
WormBase CE21017 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66107 Hypothetical protein K02F6.9 [Caenorhabditis elegans] ref|NP_494297.1| K02F6.9 [Caenorhabditis elegans] 0.0
blast CAE60681 Hypothetical protein CBG04334 [Caenorhabditis briggsae] 0.0
blast AAB66080 Hypothetical protein F18A12.1 [Caenorhabditis elegans] ref|NP_494537.1| F18A12.1 [Caenorhabditis elegans] 6.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 7.2E-96
Sequence
Target Sequence 
>C. ELEGANS:181725_AT
tctaccatatctgacgtatccgtggatggatgcggatttccctgccaacacgaattatgg
gtacctcggttacgttattggacacgagatcggacatgcatttgatcatttccaccggaa
acttgacgaaagcggcaggcaacaacagtactggttcaccgagaccaactcgtcgaatac
agagtacgagctacgggaaaaatgcctacgggatcagtattcggcgtacaaggagcccgg
ctatgacttttttatgaacgggacccactgtagatacgagatcgccggagatcatatggg
aattcggacagcttttcgggtttttgaaaaagtgaagaaagaggataggttcaggctgcc
cggcttggaaatgtcggaggaacagttgttcttctataactatgcatttcgacaatgtga
cgtgtacaacccgaataagttgccgagaaacatgcttgcccggcaacacccgacaaatcg
ataccgcgtgaacggtgtcattcagaatattccagaatttgcggaggccttcaactgccc
caaaaattcaccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTACCATATCTGACGTATCCGTGG 557 613 1533 Antisense
TGCATTTGATCATTTCCACCGGAAA 184 579 1629 Antisense
GGTTCACCGAGACCAACTCGTCGAA 375 505 1685 Antisense
TATTCGGCGTACAAGGAGCCCGGCT 614 663 1750 Antisense
GCCCGGCTATGACTTTTTTATGAAC 120 285 1767 Antisense
AACGGGACCCACTGTAGATACGAGA 387 1 1789 Antisense
GGCTGCCCGGCTTGGAAATGTCGGA 92 541 1886 Antisense
CCGAGAAACATGCTTGCCCGGCAAC 146 263 1975 Antisense
ACACCCGACAAATCGATACCGCGTG 680 109 1998 Antisense
GAATTTGCGGAGGCCTTCAACTGCC 685 325 2047 Antisense
TTCAACTGCCCCAAAAATTCACCAA 392 685 2062 Antisense
Sequence Source Affymetrix Proprietary Database