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[181713_s_at]

Description M03F4.5 /REP_DB=WormBase Gene ID /WP=CE12364 /TR=Q23015 /GB=AAB04576.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 70      6grp_highest_nonorm > 62      6grp_total > 81      4grp_total > 49      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 915
1 2 3 4 range
A 47.1
(-2.7)
A 		744.5
(2)
P 		889.2
(3)
P 		9.7
(-3)
A 		880
B 22.4
(-3)
A 		849.7
(2.5)
P 		924.2
(3)
P 		55
(-2.8)
A 		902
C 75.5
(-2.8)
A 		859.2
(2.7)
P 		895.1
(3)
P 		45
(-3)
A 		850
avr 48.3
(-2.9) 		817.8
(2.4) 		902.8
(3) 		36.6
(-3) 		866

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 513
1 2 3 4 5 6 range
A 425.6
(1.2)
P 		402.3
(0.8)
P 		467.4
(1.8)
P 		545.1
(3)
P 		424.8
(1.2)
P 		149
(-3)
P 		396
B 486.9
(1.4)
P 		381.8
(-0.6)
P 		457.4
(0.8)
P 		560.2
(2.8)
P 		568.9
(3)
P 		258
(-3)
P 		311
C 425.7
(-0)
P 		380.8
(-0.6)
P 		443.4
(0.2)
P 		539.5
(1.4)
P 		662.3
(3)
P 		194
(-3)
P 		468
avr 446.1
(1.2) 		388.3
(0.2) 		456.1
(1.4) 		548.3
(2.9) 		552
(3) 		200.3
(-3) 		352
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 17.2
(-2.6)
A 		161.6
(1.2)
P 		232.4
(3)
P 		1.4
(-3)
A 		231
B 7.2
(-3)
A 		209.9
(2.9)
P 		214.2
(3)
P 		11.7
(-2.9)
A 		207
C 25
(-2.7)
A 		247
(1.7)
P 		312
(3)
P 		7.6
(-3)
A 		304
avr 16.5
(-2.8) 		206.2
(1.9) 		252.9
(3) 		6.9
(-3) 		246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 195
1 2 3 4 5 6 range
A 78.5
(-0.6)
P 		143.1
(3)
P 		101.8
(0.7)
P 		138.3
(2.7)
P 		128.2
(2.2)
P 		36.3
(-3)
P 		107
B 169.4
(0.3)
P 		127.1
(-1.6)
P 		111.1
(-2.3)
P 		148.4
(-0.7)
P 		231.1
(3)
P 		96.2
(-3)
P 		135
C 157.8
(0.7)
P 		116.2
(-0.9)
P 		112.8
(-1.1)
P 		108.5
(-1.2)
P 		214.3
(3)
P 		64.9
(-3)
P 		149
avr 135.2
(0.3) 		128.8
(0) 		108.6
(-1) 		131.7
(0.2) 		191.2
(3) 		65.8
(-3) 		125
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181713_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16354
Sequence Type Exemplar sequence
Representative Public ID M03F4.5 NCBI
Target Description M03F4.5 /REP_DB=WormBase Gene ID /WP=CE12364 /TR=Q23015 /GB=AAB04576.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076442(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076442 NCBI Caenorhabditis elegans M03F4.6 (M03F4.6) mRNA, complete cds. 11/11 A 
GENEFINDER00000026199 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4964473:4969288:-1 11/11 None
SNAP00000026191 ENSEMBL cdna:SNAP chromosome:CEL140:X:4965789:4969288:-1 11/11 None
M03F4.6 ENSEMBL cdna:known chromosome:CEL140:X:4964391:4969326:-1 gene:M03F4.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
M03F4.2a.3 11/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4965789-4966298(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M03F4.6
Entrez Gene 180768 Entrez gene
SwissProt Q23015 EMBL-EBI
WormBase CE28621 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04576 Hypothetical protein M03F4.6 [Caenorhabditis elegans] ref|NP_508843.1| M03F4.6 [Caenorhabditis elegans] 0.0
blast CAE68671 Hypothetical protein CBG14575 [Caenorhabditis briggsae] 0.0
blast T25841 hypothetical protein M03F4.6 - Caenorhabditis elegans 5.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 1.9E-9
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 1.8E-8
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 7.4E-8
Sequence
Target Sequence 
>C. ELEGANS:181713_S_AT
caccaaagactgtaccctcgaagaagtcgctagtaacgatagatgcctggcaaaagtaca
actcggtgcccgctgctattcgaacaagcagtgcattcaatcagcagaatgtcgctttgg
aacctgtcaatgtagatgcgggtacaaacaagtcaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCAAAGACTGTACCCTCGAAGAA 507 199 87 Antisense
TGTACCCTCGAAGAAGTCGCTAGTA 704 463 97 Antisense
GTCGCTAGTAACGATAGATGCCTGG 388 451 112 Antisense
GATGCCTGGCAAAAGTACAACTCGG 394 415 128 Antisense
GGCAAAAGTACAACTCGGTGCCCGC 397 531 135 Antisense
TGCCCGCTGCTATTCGAACAAGCAG 523 583 153 Antisense
GAACAAGCAGTGCATTCAATCAGCA 552 351 168 Antisense
TCAATCAGCAGAATGTCGCTTTGGA 293 633 183 Antisense
GAATGTCGCTTTGGAACCTGTCAAT 590 331 193 Antisense
TGGAACCTGTCAATGTAGATGCGGG 292 547 204 Antisense
AGATGCGGGTACAAACAAGTCAAAG 590 67 220 Antisense
Sequence Source Affymetrix Proprietary Database