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[181711_at]

Description ZK546.4 /REP_DB=WormBase Gene ID /WP=CE02918 /TR=Q23522 /GB=AAA68737.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 42      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 10
(-3)
A 		20.3
(-1.5)
A 		51.1
(3)
A 		18.4
(-1.8)
A 		41
B 13.8
(-2.5)
A 		83.4
(3)
A 		17
(-2.2)
A 		7.4
(-3)
A 		76
C 7.3
(-3)
A 		66.1
(3)
A 		9.8
(-2.7)
A 		33.7
(-0.3)
A 		59
avr 10.4
(-3) 		56.6
(3) 		26
(-1) 		19.8
(-1.8) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 172
1 2 3 4 5 6 range
A 16.8
(-2.7)
A 		11.6
(-2.9)
A 		36.3
(-2)
A 		7.4
(-3)
A 		110.8
(0.7)
A 		176.3
(3)
M 		169
B 12.6
(-3)
A 		16.2
(-2.8)
A 		12.1
(-3)
A 		34.7
(-2)
A 		129
(2.3)
A 		143.5
(3)
A 		131
C 10.6
(-2.7)
A 		41.2
(-1.3)
A 		45.9
(-1.1)
A 		4.5
(-3)
A 		133.4
(3)
A 		128
(2.7)
A 		129
avr 13.3
(-3) 		23
(-2.6) 		31.4
(-2.2) 		15.5
(-2.9) 		124.4
(1.9) 		149.3
(3) 		136
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 3.6
(-2.5)
A 		4.4
(-2)
A 		13.4
(3)
A 		2.7
(-3)
A 		11
B 4.4
(-2.1)
A 		20.6
(3)
A 		3.9
(-2.3)
A 		1.6
(-3)
A 		19
C 2.4
(-3)
A 		19
(3)
A 		3.4
(-2.6)
A 		5.7
(-1.8)
A 		17
avr 3.5
(-2.9) 		14.7
(3) 		6.9
(-1.1) 		3.3
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 3.1
(-2.8)
A 		4.1
(-2.7)
A 		7.9
(-2.1)
A 		1.9
(-3)
A 		33.4
(1.6)
A 		42.9
(3)
M 		41
B 4.4
(-2.8)
A 		5.4
(-2.7)
A 		2.9
(-3)
A 		9.2
(-2.3)
A 		52.4
(2.9)
A 		53.5
(3)
A 		51
C 3.9
(-2.6)
A 		12.6
(-1.3)
A 		11.7
(-1.5)
A 		0.9
(-3)
A 		43.2
(3)
A 		42.8
(2.9)
A 		42
avr 3.8
(-3) 		7.4
(-2.5) 		7.5
(-2.5) 		4
(-3) 		43
(2.5) 		46.4
(3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181711_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4451
Sequence Type Exemplar sequence
Representative Public ID ZK546.4 NCBI
Target Description ZK546.4 /REP_DB=WormBase Gene ID /WP=CE02918 /TR=Q23522 /GB=AAA68737.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062503(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062503 NCBI Caenorhabditis elegans ZK546.4 (ZK546.4) mRNA, complete cds. 11/11 None
SNAP00000034946 ENSEMBL cdna:SNAP chromosome:CEL140:II:4938041:4938543:1 11/11 None
GENEFINDER00000034954 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4935548:4938543:1 11/11 None
ZK546.4 ENSEMBL cdna:known chromosome:CEL140:II:4938041:4938574:1 gene:ZK546.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4938038-4938541(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK546.4
Entrez Gene 173851 Entrez gene
SwissProt Q23522 EMBL-EBI
WormBase CE02918 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68738 Hypothetical protein ZK546.5 [Caenorhabditis elegans] ref|NP_494903.1| ZK546.5 [Caenorhabditis elegans] 0.0
blast CAE59004 Hypothetical protein CBG02280 [Caenorhabditis briggsae] 0.0
blast AAA68737 Hypothetical protein ZK546.4 [Caenorhabditis elegans] ref|NP_494904.1| ZK546.4 [Caenorhabditis elegans] 1.0E-74
blast AAA68737 Hypothetical protein ZK546.4 [Caenorhabditis elegans] ref|NP_494904.1| ZK546.4 [Caenorhabditis elegans] 4.0E-73
blast CAE59005 Hypothetical protein CBG02281 [Caenorhabditis briggsae] 4.0E-29
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494904.1 1 13-32
ZK546.4 1 13-32
SNAP00000034946 1 13-32
Sequence
Target Sequence 
>C. ELEGANS:181711_AT
caatcacaccagcagttccgaggagaagttttcgaaaagaagcgattgtgacagttgctg
ctgttggaattgcgattgttgtttggaatttcagagataaaatttctgatttttggaatt
atttggctttttcgcgtcccacaaactcggctccaacttcggaacgcgagaaaatcgatc
catccaagactgctcctgaatcatgggatgctcaaaagtctgctgacattgctcccgagt
ctgtaaaggttccagcgtcagatgagaagcatgaacagccaactgagcaacaaccaaagg
agattgtcggaaaccttgcaccggaatccaccgaaaagaatgcacccagctcatccgtct
atgttggtcttccgaaagcggatccagctaccgtatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCACACCAGCAGTTCCGAGGAG 508 179 23 Antisense
GAAGCGATTGTGACAGTTGCTGCTG 333 343 61 Antisense
CTCGGCTCCAACTTCGGAACGCGAG 276 231 168 Antisense
AAAATCGATCCATCCAAGACTGCTC 473 133 193 Antisense
GACTGCTCCTGAATCATGGGATGCT 333 375 210 Antisense
CAAAAGTCTGCTGACATTGCTCCCG 250 191 235 Antisense
TGCTCCCGAGTCTGTAAAGGTTCCA 127 575 252 Antisense
TGTCGGAAACCTTGCACCGGAATCC 681 555 327 Antisense
CGAAAAGAATGCACCCAGCTCATCC 261 253 354 Antisense
ATCCGTCTATGTTGGTCTTCCGAAA 592 37 375 Antisense
CGAAAGCGGATCCAGCTACCGTATC 565 251 395 Antisense
Sequence Source Affymetrix Proprietary Database