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[181708_s_at]

Description K10B2.5 /REP_DB=WormBase Gene ID /WP=CE02012 /TR=Q09994 /GB=AAA68262.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 88      4grp_high_8_8 > 38      6grp_total > 88      4grp_total > 33      6grp_cgc > 59      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 106.5
(-3)
P 		121.2
(0.6)
M 		130.9
(3)
P 		119.2
(0.1)
A 		24
B 76.1
(-3)
P 		187.3
(3)
P 		110.7
(-1.1)
P 		142.7
(0.6)
P 		111
C 62.8
(-3)
P 		114.6
(1.2)
P 		136.1
(3)
P 		119.5
(1.6)
P 		73
avr 81.8
(-3) 		141
(3) 		125.9
(1.5) 		127.1
(1.6) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 418
1 2 3 4 5 6 range
A 73.9
(-3)
A 		98.3
(-2.6)
P 		111.8
(-2.4)
P 		99.2
(-2.6)
P 		268.2
(-0.1)
P 		478.9
(3)
P 		405
B 102
(-2.4)
P 		72.6
(-2.8)
P 		61.3
(-3)
M 		141.7
(-1.7)
P 		191.6
(-1)
P 		444.3
(3)
P 		383
C 112.2
(-2.2)
P 		82.5
(-3)
P 		114.7
(-2.2)
P 		114.6
(-2.2)
P 		313.6
(3)
P 		298.3
(2.6)
P 		231
avr 96
(-2.8) 		84.5
(-3) 		95.9
(-2.8) 		118.5
(-2.4) 		257.8
(0.2) 		407.2
(3) 		323
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 38.8
(3)
P 		26.3
(-0.5)
M 		34.2
(1.7)
P 		17.4
(-3)
A 		21
B 24.3
(-3)
P 		46.3
(3)
P 		25.7
(-2.6)
P 		30.4
(-1.3)
P 		22
C 20.8
(-2.8)
P 		32.9
(-0.2)
P 		47.4
(3)
P 		20.1
(-3)
P 		27
avr 28
(-0.5) 		35.2
(2.7) 		35.8
(3) 		22.6
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 13.6
(-3)
A 		34.9
(-1.8)
P 		24.3
(-2.4)
P 		25.2
(-2.3)
P 		80.9
(0.9)
P 		116.6
(3)
P 		103
B 35.5
(-2.2)
P 		24.2
(-2.6)
P 		14.9
(-3)
M 		37.5
(-2.1)
P 		77.9
(-0.5)
P 		165.7
(3)
P 		151
C 41.6
(-1.6)
P 		25.2
(-2.8)
P 		29.2
(-2.5)
P 		23
(-3)
P 		101.5
(3)
P 		99.9
(2.9)
P 		79
avr 30.2
(-2.6) 		28.1
(-2.7) 		22.8
(-3) 		28.6
(-2.7) 		86.8
(0.7) 		127.4
(3) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181708_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4440
Sequence Type Exemplar sequence
Representative Public ID K10B2.5 NCBI
Target Description K10B2.5 /REP_DB=WormBase Gene ID /WP=CE02012 /TR=Q09994 /GB=AAA68262.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062881(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062881 NCBI Caenorhabditis elegans ANIllin (actin binding protein) family member (ani-2) (ani-2) mRNA, complete cds. 11/11 None
SNAP00000015730 ENSEMBL cdna:SNAP chromosome:CEL140:II:6361543:6366604:-1 11/11 None
GENEFINDER00000015737 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6361543:6365226:-1 11/11 None
K10B2.5 ENSEMBL cdna:known chromosome:CEL140:II:6361134:6365251:-1 gene:K10B2.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6361539-6365223(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ani-2
Ensembl K10B2.5
Entrez Gene 174055 Entrez gene
SwissProt Q09994 EMBL-EBI
WormBase CE02012 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68262 Hypothetical protein K10B2.5 [Caenorhabditis elegans] ref|NP_495282.1| ANIllin (actin binding protein) family member (ani-2) [Caenorhabditis elegans] 0.0
blast CAE59303 Hypothetical protein CBG02638 [Caenorhabditis briggsae] 0.0
blast CAA21615 Hypothetical protein Y43F8C.14 [Caenorhabditis elegans] ref|NP_507814.1| ANIllin (actin binding protein) family member (ani-3) [Caenorhabditis elegans] 1.0E-158
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.1E-19
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.1E-19
Sequence
Target Sequence 
>C. ELEGANS:181708_S_AT
catgtgcagcagtattggcggccaagttccggaatttgttggttcccaactctgcccatc
gtcgtgcgagaaacaacagtgtcctgtcaaatcgaacagttctctcgatgccaggatacc
aggctccagactgcgacttcagattctgtggaaagctgacacttgatcgcgactccgccg
gcgatcgtcagttctatctcgatgatgtgacgtatccactggaaggaactgtgaagcttc
aatctcactgctcttcgcttccagaagcaattgacgtcgaatatcgtgggtttctgtatt
tgttcgatgagcgatctccaaatggcgaagcagcgtgggacagatattgggcaatgctcc
accgtggaatcatcttcttttggaaaaatcctattgatgagaagaatcaaaaagtgccac
tgtctcaaattgatttgaccaaatgcacaaatcagtcggtcgaagaaacaagaaacggac
gagaccacgaattccatatcgaactgctcattgatcagacacccagtcttctcgagaaaa
gacgagttgtgctagccgcggaatcttctgatcatcttgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATGTGCAGCAGTATTGGCGGCCAA 86 211 2414 Antisense
GGCGGCCAAGTTCCGGAATTTGTTG 522 539 2430 Antisense
TCTGCCCATCGTCGTGCGAGAAACA 316 609 2464 Antisense
GATGCCAGGATACCAGGCTCCAGAC 252 415 2520 Antisense
GAAAGCTGACACTTGATCGCGACTC 462 359 2564 Antisense
GGCGATCGTCAGTTCTATCTCGATG 450 541 2593 Antisense
TGAAGCTTCAATCTCACTGCTCTTC 240 575 2645 Antisense
TCCACCGTGGAATCATCTTCTTTTG 556 587 2771 Antisense
AAAGTGCCACTGTCTCAAATTGATT 610 121 2824 Antisense
TCAGACACCCAGTCTTCTCGAGAAA 11 625 2928 Antisense
GCGGAATCTTCTGATCATCTTGCTT 646 293 2971 Antisense
Sequence Source Affymetrix Proprietary Database