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[181702_at]

Description ZK355.2 /REP_DB=WormBase Gene ID /WP=CE24723 /TR=Q9N4P1 /GB=AAF40001.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 08      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 71      6grp_total > 09      4grp_total > 10      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 429
1 2 3 4 range
A 425.8
(-1.5)
P 		435.1
(-1.2)
P 		358.7
(-3)
P 		619.8
(3)
P 		261
B 355.8
(-3)
P 		382.9
(-2.6)
P 		441.7
(-1.6)
P 		720.7
(3)
P 		365
C 368.3
(-1.4)
P 		415.6
(-0.4)
P 		291.4
(-3)
P 		576.4
(3)
P 		285
avr 383.3
(-2.6) 		411.2
(-2) 		363.9
(-3) 		639
(3) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 266
1 2 3 4 5 6 range
A 514.6
(3)
P 		347.4
(-2.4)
P 		341.4
(-2.6)
P 		425.6
(0.1)
P 		380
(-1.3)
P 		328.8
(-3)
P 		186
B 454.6
(3)
P 		316
(-2.2)
P 		348.2
(-1)
P 		422.2
(1.8)
P 		428.4
(2)
P 		293.6
(-3)
P 		161
C 383.5
(3)
P 		346.9
(1.4)
P 		348.9
(1.5)
P 		354.7
(1.7)
P 		308.9
(-0.3)
P 		248.4
(-3)
P 		135
avr 450.9
(3) 		336.8
(-1.3) 		346.2
(-0.9) 		400.8
(1.1) 		372.4
(0.1) 		290.3
(-3) 		161
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 155
(3)
P 		94.4
(-2.7)
P 		93.8
(-2.7)
P 		90.7
(-3)
P 		64
B 113.5
(-1.1)
P 		94.6
(-3)
P 		102.3
(-2.2)
P 		153.6
(3)
P 		59
C 121.9
(3)
P 		119.5
(2.4)
P 		101.6
(-1.9)
P 		97.2
(-3)
P 		25
avr 130.1
(3) 		102.8
(-2.3) 		99.2
(-3) 		113.8
(-0.2) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 94.9
(-0.5)
P 		123.5
(3)
P 		74.3
(-3)
P 		107.9
(1.1)
P 		114.7
(1.9)
P 		80.1
(-2.3)
P 		49
B 158.2
(1.9)
P 		105.2
(-1.6)
P 		84.6
(-3)
P 		111.8
(-1.2)
P 		174.1
(3)
P 		109.5
(-1.3)
P 		90
C 142.1
(3)
P 		105.9
(-0.1)
P 		88.8
(-1.5)
P 		71.3
(-3)
P 		99.9
(-0.6)
P 		83.2
(-2)
P 		71
avr 131.7
(3) 		111.5
(0.5) 		82.6
(-3) 		97
(-1.2) 		129.6
(2.7) 		90.9
(-2) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181702_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4720
Sequence Type Exemplar sequence
Representative Public ID ZK355.2 NCBI
Target Description ZK355.2 /REP_DB=WormBase Gene ID /WP=CE24723 /TR=Q9N4P1 /GB=AAF40001.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061999(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061999 NCBI Caenorhabditis elegans ZK355.2a (ZK355.2) mRNA, complete cds. 10/11 None
ZK355.2a ENSEMBL cdna:known chromosome:CEL140:II:2923907:2926518:1 gene:ZK355.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182219 8/11 Cross Hyb Matching Probes None
SNAP00000021470 8/11 Cross Hyb Matching Probes None
GENEFINDER00000021481 8/11 Cross Hyb Matching Probes None
ZK355.2b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173640 2 1 NM_061999 181702_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2923931-2926267(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK355.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO38580 Hypothetical protein ZK355.2a [Caenorhabditis elegans] ref|NP_494400.2| ZK355.2a [Caenorhabditis elegans] 0.0
blast AAO38581 Hypothetical protein ZK355.2b [Caenorhabditis elegans] ref|NP_872019.1| ZK355.2b [Caenorhabditis elegans] 0.0
blast CAE72451 Hypothetical protein CBG19621 [Caenorhabditis briggsae] 1.0E-157
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494400.2 7 53-75,88-107,111-133,153-175,185-207,228-250,277-295
ZK355.2a 7 53-75,88-107,111-133,153-175,185-207,228-250,277-295
Sequence
Target Sequence 
>C. ELEGANS:181702_AT
gctcgtctcattctgtatgatcctccgccgaatgaatcgaatctcggcgcctgtccaaat
gcgagccgcccaacgtcgggacgtctttgtcctgatgtgggcattcgagacggcgacttt
ctgcgatctagcttatcacgtcgggatgttcatgttggccgatgagcacgccgcgaatag
ttgctccgcgatcttttaccacgatcaactgcgctacaccgcactcaaggctccgtacga
ctttatcaactttatcatgccagtctgggcgatcctgtatgtgttccgatcgaagaccaa
gctgagatcggatagtgatgaggattcggcagagtcatcaaccgcctcctccacctggtc
ctccaaccactcccgaaacaacgtccgaatcgccgacgtgataaccgtccctgcagcggc
acatcccatcccaaacatgctctccccaccaccatctgctgctccagcggcaccccgact
ccgaagtgtgtcatcagcccctatgatccgccggatcagtgtctcacactccattgtgag
ctccccgctgtacagtataccagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGTCTCATTCTGTATGATCCTC 609 299 612 Antisense
TATGATCCTCCGCCGAATGAATCGA 632 653 627 Antisense
AACGTCGGGACGTCTTTGTCCTGAT 443 137 683 Antisense
GACTTTCTGCGATCTAGCTTATCAC 144 371 726 Antisense
GAATAGTTGCTCCGCGATCTTTTAC 199 327 786 Antisense
TTTACCACGATCAACTGCGCTACAC 570 671 806 Antisense
AGTCTGGGCGATCCTGTATGTGTTC 593 59 873 Antisense
ATTCGGCAGAGTCATCAACCGCCTC 412 9 935 Antisense
AATCGCCGACGTGATAACCGTCCCT 12 123 999 Antisense
CGACTCCGAAGTGTGTCATCAGCCC 631 251 1087 Antisense
CTCCCCGCTGTACAGTATACCAGAG 295 233 1152 Antisense
Sequence Source Affymetrix Proprietary Database