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[181700_at]

Description T25B6.5 /REP_DB=WormBase Gene ID /WP=CE05015 /TR=Q22760 /GB=AAC48221.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 18      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 7.4
(-3)
A 		19.1
(-0.4)
A 		10.1
(-2.4)
A 		34
(3)
A 		27
B 8.9
(0.9)
A 		5.5
(-1.6)
A 		3.6
(-3)
A 		11.7
(3)
A 		8
C 8.4
(0.2)
A 		7.1
(-1.5)
A 		10.5
(3)
A 		6
(-3)
A 		5
avr 8.2
(-2.9) 		10.6
(-1.4) 		8.1
(-3) 		17.2
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 10.7
(3)
A 		5.4
(-1.5)
A 		9.1
(1.6)
A 		3.7
(-3)
A 		5
(-1.9)
A 		5.9
(-1.1)
A 		7
B 15
(3)
A 		13.4
(2.1)
A 		9.1
(-0.3)
A 		9.8
(0.1)
A 		4.2
(-3)
A 		5.8
(-2.1)
A 		11
C 25.1
(2.2)
A 		28.2
(3)
A 		5.6
(-2.8)
A 		5.4
(-2.8)
A 		4.9
(-3)
A 		4.8
(-3)
A 		23
avr 16.9
(3) 		15.7
(2.4) 		7.9
(-1.4) 		6.3
(-2.2) 		4.7
(-3) 		5.5
(-2.6) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 2.7
(-2.8)
A 		4.2
(1)
A 		2.6
(-3)
A 		5
(3)
A 		2
B 2.8
(3)
A 		1.4
(-1.2)
A 		0.8
(-3)
A 		2.5
(2.1)
A 		2
C 2.8
(1)
A 		2
(-0.8)
A 		3.7
(3)
A 		1
(-3)
A 		3
avr 2.8
(3) 		2.5
(-1.5) 		2.4
(-3) 		2.8
(3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 2
(3)
A 		1.9
(2.5)
A 		2
(3)
A 		0.9
(-3)
A 		1.5
(0.3)
A 		1.4
(-0.3)
A 		1
B 5.2
(3)
A 		4.5
(1.8)
A 		2.2
(-2.1)
A 		2.6
(-1.5)
A 		1.7
(-3)
A 		2.2
(-2.1)
A 		4
C 9.3
(3)
A 		8.6
(2.5)
A 		1.4
(-2.8)
A 		1.1
(-3)
A 		1.6
(-2.6)
A 		1.6
(-2.6)
A 		8
avr 5.5
(3) 		5
(2.3) 		1.9
(-2.4) 		1.5
(-3) 		1.6
(-2.9) 		1.7
(-2.7) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181700_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16907
Sequence Type Exemplar sequence
Representative Public ID T25B6.5 NCBI
Target Description T25B6.5 /REP_DB=WormBase Gene ID /WP=CE05015 /TR=Q22760 /GB=AAC48221.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077123(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077123 NCBI Caenorhabditis elegans T25B6.5 (T25B6.5) mRNA, complete cds. 11/11 None
SNAP00000006428 ENSEMBL cdna:SNAP chromosome:CEL140:X:9020523:9022269:-1 11/11 None
GENEFINDER00000006434 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9020523:9021654:-1 11/11 None
T25B6.5 ENSEMBL cdna:known chromosome:CEL140:X:9020523:9021535:-1 gene:T25B6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9020521-9021534(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25B6.5
Entrez Gene 188877 Entrez gene
SwissProt Q22760 EMBL-EBI
WormBase CE05015 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509524 T25B6.5 [Caenorhabditis elegans] gb|AAC48221.1| Hypothetical protein T25B6.5 [Caenorhabditis elegans] 4.0E-49
blast NP_509524 T25B6.5 [Caenorhabditis elegans] gb|AAC48221.1| Hypothetical protein T25B6.5 [Caenorhabditis elegans] 1.0E-48
blast NP_509525 T25B6.6 [Caenorhabditis elegans] gb|AAC48220.2| Hypothetical protein T25B6.6 [Caenorhabditis elegans] 1.0E-36
blast CAE57303 Hypothetical protein CBG00220 [Caenorhabditis briggsae] 2.0E-27
blast CAE57303 Hypothetical protein CBG00220 [Caenorhabditis briggsae] 7.0E-27
blast CAE57303 Hypothetical protein CBG00220 [Caenorhabditis briggsae] 8.0E-18
Sequence
Target Sequence 
>C. ELEGANS:181700_AT
agtggaaccgggagcgtcgattgactctgcataactttgatgcaatggttctaaccgcgg
aaggaaataatgaggaggccaaagattccctcctgactcttctgaacacttggctttttc
gtgttcgttttgcccaaaagctcgccgagcttggtgtcttcatcttgtttgacggacctg
gacgtttgctcccaatacccggattctttgtgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGGAACCGGGAGCGTCGATTGAC 258 57 50 Antisense
GTCGATTGACTCTGCATAACTTTGA 507 473 65 Antisense
GATGCAATGGTTCTAACCGCGGAAG 564 411 88 Antisense
GGAGGCCAAAGATTCCCTCCTGACT 564 513 123 Antisense
TGACTCTTCTGAACACTTGGCTTTT 452 571 143 Antisense
GAACACTTGGCTTTTTCGTGTTCGT 387 351 153 Antisense
CGTGTTCGTTTTGCCCAAAAGCTCG 15 3 169 Antisense
AAAGCTCGCCGAGCTTGGTGTCTTC 564 123 186 Antisense
GTGTCTTCATCTTGTTTGACGGACC 676 485 203 Antisense
GTTTGACGGACCTGGACGTTTGCTC 708 431 216 Antisense
TCCCAATACCCGGATTCTTTGTGCA 177 597 239 Antisense
Sequence Source Affymetrix Proprietary Database