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[181699_at]

Description F58E1.3 /REP_DB=WormBase Gene ID /WP=CE11376 /TR=Q9TZG6 /GB=AAC67480.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 18      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 16.5
(-2.3)
A 		7.6
(-3)
A 		14.7
(-2.4)
A 		82
(3)
P 		74
B 2.4
(-3)
A 		47.1
(1.9)
A 		40.8
(1.2)
A 		56.9
(3)
A 		55
C 40.8
(0.2)
A 		56.8
(1.8)
A 		8.8
(-3)
A 		68.4
(3)
P 		60
avr 19.9
(-3) 		37.2
(-0.9) 		21.4
(-2.8) 		69.1
(3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 10.8
(-2.4)
A 		1.9
(-3)
A 		7.4
(-2.6)
A 		4.6
(-2.8)
A 		30.3
(-1.1)
A 		92.5
(3)
A 		91
B 28.8
(-0.6)
A 		10.2
(-2.5)
A 		11.1
(-2.4)
A 		4.8
(-3)
A 		8.9
(-2.6)
A 		65.2
(3)
A 		60
C 4.8
(-3)
A 		52.5
(2.3)
A 		26.4
(-0.6)
A 		5.4
(-2.9)
A 		22
(-1.1)
A 		58.5
(3)
A 		54
avr 14.8
(-2.1) 		21.5
(-1.5) 		15
(-2.1) 		4.9
(-3) 		20.4
(-1.6) 		72.1
(3) 		67
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 6
(-0.5)
A 		1.6
(-3)
A 		3.8
(-1.7)
A 		12
(3)
P 		10
B 0.8
(-3)
A 		11.6
(2.7)
A 		9.5
(1.6)
A 		12.1
(3)
A 		11
C 13.5
(1.7)
A 		16.3
(3)
A 		3.1
(-3)
A 		11.5
(0.8)
P 		13
avr 6.8
(-1.8) 		9.8
(1) 		5.5
(-3) 		11.9
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 2
(-2.6)
A 		0.7
(-3)
A 		1.6
(-2.8)
A 		1.2
(-2.9)
A 		9.1
(-0.7)
A 		22.5
(3)
A 		22
B 10
(-0.7)
A 		3.4
(-2.5)
A 		2.7
(-2.6)
A 		1.3
(-3)
A 		3.6
(-2.4)
A 		24.3
(3)
A 		23
C 1.8
(-2.8)
A 		16
(1.8)
A 		6.7
(-1.2)
A 		1.1
(-3)
A 		7.1
(-1.1)
A 		19.6
(3)
A 		19
avr 4.6
(-2) 		6.7
(-1.4) 		3.7
(-2.3) 		1.2
(-3) 		6.6
(-1.4) 		22.1
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181699_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5236
Sequence Type Exemplar sequence
Representative Public ID F58E1.3 NCBI
Target Description F58E1.3 /REP_DB=WormBase Gene ID /WP=CE11376 /TR=Q9TZG6 /GB=AAC67480.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061638(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061638 NCBI Caenorhabditis elegans F58E1.3 (F58E1.3) mRNA, complete cds. 11/11 None
GENEFINDER00000040833 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1682542:1683527:1 11/11 None
F58E1.3 ENSEMBL cdna:known chromosome:CEL140:II:1682542:1683527:1 gene:F58E1.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000040825 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1682540-1683526(+) 97.54 100.0
Public Domain and Genome References
Gene Title
Ensembl F58E1.3
Entrez Gene 186508 Entrez gene
SwissProt Q9TZG6 EMBL-EBI
WormBase CE11376 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67480 Hypothetical protein F58E1.3 [Caenorhabditis elegans] ref|NP_494039.1| F58E1.3 [Caenorhabditis elegans] 1.0E-179
blast AAC67481 Hypothetical protein F58E1.2 [Caenorhabditis elegans] ref|NP_494040.1| F58E1.2 [Caenorhabditis elegans] 2.0E-71
blast NP_494204 C33C12.5 [Caenorhabditis elegans] gb|AAB66057.1| Hypothetical protein C33C12.5 [Caenorhabditis elegans] 9.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.0E-6
Sequence
Target Sequence 
>C. ELEGANS:181699_AT
tggaacctttttcttcacaaattcaagattaccagcgtctcacactttttccttctttgg
aatggttgacagtgattggagtggacgaggctccaattggtactccaatcctaattcaga
atttcgaaggattcataatttttcctaacatacaatggaaaatcgatgatgtactaattt
caaatttttcgaatttgcgcatgtggcaccgaacattttccgtccaagacctgaacctct
tcatgagacagtggatcaagggatcaatgccccgtctcgaagaattcgacgtaatccttt
cccactctaatcaacacaaacttgtggaaactgacctattcaaaagtatcaagtacactg
tcaaagaaaacgagaattgcaaaatcaatgtaatcaagagacgcgatggagcaaaagcga
gcgtctgttttggtggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAACCTTTTTCTTCACAAATTCA 609 545 464 Antisense
TCAAGATTACCAGCGTCTCACACTT 160 633 486 Antisense
CACACTTTTTCCTTCTTTGGAATGG 674 193 504 Antisense
GGACGAGGCTCCAATTGGTACTCCA 433 523 546 Antisense
GAATTTGCGCATGTGGCACCGAACA 420 325 654 Antisense
GAACATTTTCCGTCCAAGACCTGAA 504 351 674 Antisense
GACCTGAACCTCTTCATGAGACAGT 177 383 691 Antisense
GATCAAGGGATCAATGCCCCGTCTC 145 423 717 Antisense
CAATGCCCCGTCTCGAAGAATTCGA 49 175 728 Antisense
CTTTCCCACTCTAATCAACACAAAC 309 223 760 Antisense
AAAAGCGAGCGTCTGTTTTGGTGGG 503 129 876 Antisense
Sequence Source Affymetrix Proprietary Database