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[181691_at]

Description C25H3.4 /REP_DB=WormBase Gene ID /WP=CE27093 /TR=Q18186 /GB=AAK31455.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_high_8_8 > 59      6grp_highest_nonorm > 81      6grp_total > 75      4grp_total > 60      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 372
1 2 3 4 range
A 307.8
(1.9)
P 		228.6
(-0.3)
P 		347.8
(3)
P 		134.1
(-3)
A 		214
B 294
(2)
P 		241.1
(-0.4)
P 		315.2
(3)
P 		183.2
(-3)
P 		132
C 307.8
(-0.6)
P 		300.8
(-0.7)
P 		505.8
(3)
P 		176.4
(-3)
P 		329
avr 303.2
(0.7) 		256.8
(-0.5) 		389.6
(3) 		164.6
(-3) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 240.8
(-1.8)
P 		264.2
(-0.9)
P 		294.8
(0.1)
P 		375.3
(3)
P 		206.5
(-3)
P 		284.3
(-0.2)
P 		169
B 246.5
(-1.7)
P 		217.6
(-3)
P 		233.4
(-2.3)
P 		275.6
(-0.3)
P 		290.7
(0.4)
P 		347.1
(3)
P 		130
C 237.9
(-3)
P 		271.3
(-1.3)
P 		325.5
(1.3)
P 		337
(1.9)
P 		276.1
(-1.1)
P 		358.9
(3)
P 		121
avr 241.7
(-3) 		251
(-2.4) 		284.6
(-0.1) 		329.3
(2.9) 		257.8
(-1.9) 		330.1
(3) 		88
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 112.1
(3)
P 		49.6
(-1.1)
P 		90.9
(1.6)
P 		19.6
(-3)
A 		93
B 93.7
(3)
P 		59.5
(-0.8)
P 		73
(0.7)
P 		39
(-3)
P 		55
C 101.9
(-0)
P 		86.5
(-0.7)
P 		176.3
(3)
P 		29.7
(-3)
P 		147
avr 102.6
(2.2) 		65.2
(-0.4) 		113.4
(3) 		29.4
(-3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 44.4
(-3)
P 		93.9
(2.8)
P 		64.2
(-0.7)
P 		95.2
(3)
P 		62.3
(-0.9)
P 		69.2
(-0.1)
P 		51
B 85.8
(-0.6)
P 		72.4
(-1.7)
P 		56.7
(-3)
P 		73
(-1.7)
P 		118.1
(2.1)
P 		129.4
(3)
P 		73
C 88.2
(-0.7)
P 		82.8
(-1.3)
P 		82.8
(-1.3)
P 		67.8
(-3)
P 		89.3
(-0.5)
P 		120.1
(3)
P 		52
avr 72.8
(-2.2) 		83
(-0.6) 		67.9
(-3) 		78.7
(-1.3) 		89.9
(0.4) 		106.2
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181691_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4567
Sequence Type Exemplar sequence
Representative Public ID C25H3.4 NCBI
Target Description C25H3.4 /REP_DB=WormBase Gene ID /WP=CE27093 /TR=Q18186 /GB=AAK31455.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062713(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062713 NCBI Caenorhabditis elegans C25H3.4 (C25H3.4) mRNA, complete cds. 11/11 None
SNAP00000016808 ENSEMBL cdna:SNAP chromosome:CEL140:II:5690532:5693730:1 11/11 None
GENEFINDER00000016822 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5691698:5693730:1 11/11 None
C25H3.4 ENSEMBL cdna:known chromosome:CEL140:II:5691689:5693891:1 gene:C25H3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5691243-5693727(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25H3.4
Entrez Gene 173964 Entrez gene
SwissProt Q18186 EMBL-EBI
WormBase CE29676 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6413 translational initiation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31455 Hypothetical protein C25H3.4 [Caenorhabditis elegans] ref|NP_495114.2| C25H3.4 [Caenorhabditis elegans] 0.0
blast T15629 hypothetical protein C25H3.4 - Caenorhabditis elegans 0.0
blast CAE59172 Hypothetical protein CBG02479 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001950 EMBL-EBI Translation initiation factor SUI1 1.3E-7
Pfam IPR001950 EMBL-EBI Translation initiation factor SUI1 1.3E-7
Pfam IPR001950 EMBL-EBI Translation initiation factor SUI1 1.3E-7
Sequence
Target Sequence 
>C. ELEGANS:181691_AT
agaaggccaagtttgatgctcccgtgatagctgaatattttgcaattaccgaaccaacgc
tgaagttgttccctggctgctcaaaaggggatcttctgactgtgaaacaaatcaaagaat
ttgtcacaaaatatgtaaatgaaaacaaacttgctgctggaggatcagttcgtcttgatc
caattatcttttcggtcactaaaatccaaacggatacaactccatgggcaaatttgatga
aagaaatccattcaagaatgactgcaacatggcacataagatggcctgacggcagagaaa
tagtcagaaaagtatctccaccgagagttgaattcaagatcgaaaaccgtgctggaaaca
aaaaagttactcttttaaatggattagcgatgtttggaattgacattcgaacgatttgcc
atcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGGCCAAGTTTGATGCTCCCGT 477 73 941 Antisense
GATGCTCCCGTGATAGCTGAATATT 103 415 955 Antisense
TTTGCAATTACCGAACCAACGCTGA 250 667 979 Antisense
CCAACGCTGAAGTTGTTCCCTGGCT 74 277 994 Antisense
GTTGTTCCCTGGCTGCTCAAAAGGG 521 435 1005 Antisense
AAAACAAACTTGCTGCTGGAGGATC 350 117 1082 Antisense
GATCCAATTATCTTTTCGGTCACTA 590 415 1117 Antisense
CCAAACGGATACAACTCCATGGGCA 559 275 1146 Antisense
GGCACATAAGATGGCCTGACGGCAG 619 535 1211 Antisense
GTCAGAAAAGTATCTCCACCGAGAG 555 463 1243 Antisense
TGACATTCGAACGATTTGCCATCAA 627 571 1341 Antisense
Sequence Source Affymetrix Proprietary Database