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[181681_at]

Description F08F1.6 /REP_DB=WormBase Gene ID /WP=CE09253 /TR=O17387 /GB=AAB71306.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_high_8_8 > 47      6grp_highest_nonorm > 54      6grp_total > 81      4grp_total > 48      6grp_highest_sub > 04_05      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1610
1 2 3 4 range
A 49.6
(-3)
P 		1164.1
(1.2)
P 		1641.9
(3)
P 		501.2
(-1.3)
P 		1592
B 47.7
(-3)
A 		1090.3
(2.4)
P 		1197.1
(3)
P 		586.2
(-0.2)
P 		1149
C 39
(-3)
A 		1043.8
(0.7)
P 		1648.5
(3)
P 		506.8
(-1.3)
P 		1610
avr 45.4
(-3) 		1099.4
(1.4) 		1495.8
(3) 		531.4
(-1) 		1450

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2331
1 2 3 4 5 6 range
A 130.8
(-3)
P 		774.4
(-1.3)
P 		1427.2
(0.3)
P 		2461.4
(3)
P 		1654.1
(0.9)
P 		424.6
(-2.2)
P 		2331
B 149.4
(-3)
P 		884.6
(-0.7)
P 		948.3
(-0.5)
P 		2091.1
(3)
P 		2030.6
(2.8)
P 		505.1
(-1.9)
P 		1942
C 153.4
(-3)
P 		878.7
(-0.9)
P 		1045.8
(-0.4)
P 		2214.8
(3)
P 		1937.4
(2.2)
P 		412.4
(-2.2)
P 		2061
avr 144.5
(-3) 		845.9
(-1) 		1140.4
(-0.2) 		2255.8
(3) 		1874
(1.9) 		447.4
(-2.1) 		2111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 562
1 2 3 4 range
A 18
(-3)
P 		252.7
(0.4)
P 		429.2
(3)
P 		73.4
(-2.2)
P 		411
B 15.2
(-3)
A 		269.3
(2.8)
P 		277.4
(3)
P 		124.9
(-0.5)
P 		262
C 12.9
(-3)
A 		300.1
(0.1)
P 		574.6
(3)
P 		85.5
(-2.2)
P 		562
avr 15.4
(-3) 		274
(0.8) 		427.1
(3) 		94.6
(-1.8) 		412

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 801
1 2 3 4 5 6 range
A 24.1
(-3)
P 		275.4
(-0.5)
P 		310.8
(-0.1)
P 		624.3
(3)
P 		499.1
(1.7)
P 		103.4
(-2.2)
P 		600
B 52
(-3)
P 		294.5
(-1.1)
P 		230.3
(-1.6)
P 		553.9
(0.9)
P 		825
(3)
P 		188.4
(-1.9)
P 		773
C 56.9
(-3)
P 		268.1
(-0.8)
P 		266.2
(-0.8)
P 		445.4
(1.1)
P 		626.9
(3)
P 		138
(-2.1)
P 		570
avr 44.3
(-3) 		279.3
(-0.7) 		269.1
(-0.8) 		541.2
(1.9) 		650.3
(3) 		143.3
(-2) 		606
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181681_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16420
Sequence Type Exemplar sequence
Representative Public ID F08F1.6 NCBI
Target Description F08F1.6 /REP_DB=WormBase Gene ID /WP=CE09253 /TR=O17387 /GB=AAB71306.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077027(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077027 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-13) (spp-13) mRNA, complete cds. 11/11 None
SNAP00000012387 ENSEMBL cdna:SNAP chromosome:CEL140:X:8417516:8418326:-1 11/11 None
GENEFINDER00000012395 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8417516:8418326:-1 10/11 None
F08F1.6 ENSEMBL cdna:known chromosome:CEL140:X:8417516:8418326:-1 gene:F08F1.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8417514-8418325(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-13
Ensembl F08F1.6
Entrez Gene 184191 Entrez gene
SwissProt O17387 EMBL-EBI
WormBase CE09253 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71306 Saposin-like protein family protein 13 [Caenorhabditis elegans] ref|NP_509428.1| SaPosin-like Protein family member (spp-13) [Caenorhabditis elegans] 8.0E-68
blast AAB71306 Saposin-like protein family protein 13 [Caenorhabditis elegans] ref|NP_509428.1| SaPosin-like Protein family member (spp-13) [Caenorhabditis elegans] 1.0E-64
blast CAE74897 Hypothetical protein CBG22763 [Caenorhabditis briggsae] 2.0E-55
blast CAE74897 Hypothetical protein CBG22763 [Caenorhabditis briggsae] 7.0E-55
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509428.1 1 5-24
F08F1.6 1 5-24
SNAP00000012387 1 5-24
GENEFINDER00000012395 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:181681_AT
ttgttgacttttgttttaattgctttaatggtttcggttacttttgctgaaattattcaa
aaaggaaagttggctaaacatggaatgctgaaagaaaacaagccaaattgccttttgacc
agatcccgtcttgggtgcacatgcacaacgtgtaaagaaatagtcaatttcactagaatg
ctaattttgaaccatgtgccggaggagcaagaagttatggagaaagtctgctatagaatc
tttggtgatgacaagaagaaggaatctttctgtgaggagctaataaaggaagagcttccc
gatatcatcaagtatgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTTGACTTTTGTTTTAATTGCTT 174 687 28 Antisense
TAATGGTTTCGGTTACTTTTGCTGA 168 599 53 Antisense
ACAAGCCAAATTGCCTTTTGACCAG 83 113 125 Antisense
TTGACCAGATCCCGTCTTGGGTGCA 350 703 142 Antisense
TCCCGTCTTGGGTGCACATGCACAA 130 597 151 Antisense
GGTGCACATGCACAACGTGTAAAGA 446 501 161 Antisense
GCTAATTTTGAACCATGTGCCGGAG 292 301 207 Antisense
AAGTCTGCTATAGAATCTTTGGTGA 29 159 251 Antisense
AAGGAATCTTTCTGTGAGGAGCTAA 650 149 286 Antisense
GGAAGAGCTTCCCGATATCATCAAG 525 531 315 Antisense
GCTTCCCGATATCATCAAGTATGTC 628 305 321 Antisense
Sequence Source Affymetrix Proprietary Database