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[181677_at]

Description F40H3.4 /REP_DB=WormBase Gene ID /WP=CE19865 /TR=Q9TZK1 /GB=AAC67430.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 60      6grp_total > 24      4grp_total > 21      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 670
1 2 3 4 range
A 529.7
(0.9)
P 		334.4
(-1.3)
P 		174.2
(-3)
P 		726.1
(3)
P 		552
B 542.9
(0.5)
P 		354.1
(-1.5)
P 		209.9
(-3)
P 		773.4
(3)
P 		564
C 517.1
(0)
P 		295.4
(-2)
P 		180.4
(-3)
P 		844.6
(3)
P 		664
avr 529.9
(0.5) 		328
(-1.6) 		188.2
(-3) 		781.4
(3) 		593

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 344
1 2 3 4 5 6 range
A 544.5
(3)
P 		408.1
(0.4)
P 		461.3
(1.4)
P 		317.1
(-1.3)
P 		228.6
(-3)
P 		282.2
(-2)
P 		316
B 542
(3)
P 		437.6
(1)
P 		443.9
(1.1)
P 		278.6
(-2)
P 		224.6
(-3)
P 		312.9
(-1.3)
P 		317
C 451
(2.5)
P 		474.3
(3)
P 		331.5
(-0.1)
P 		353
(0.3)
P 		200.8
(-3)
P 		277.2
(-1.3)
P 		274
avr 512.5
(3) 		440
(1.5) 		412.2
(1) 		316.2
(-1) 		218
(-3) 		290.8
(-1.5) 		295
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 192.9
(3)
P 		72.6
(-1.9)
P 		45.5
(-3)
P 		106.3
(-0.5)
P 		147
B 173.1
(3)
P 		87.5
(-1.1)
P 		48.6
(-3)
P 		164.8
(2.6)
P 		125
C 171.2
(3)
P 		84.9
(-1.8)
P 		62.9
(-3)
P 		142.4
(1.4)
P 		108
avr 179.1
(3) 		81.7
(-1.6) 		52.3
(-3) 		137.8
(1) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 100.4
(-0.5)
P 		145.1
(3)
P 		100.5
(-0.5)
P 		80.4
(-2.1)
P 		69
(-3)
P 		68.7
(-3)
P 		76
B 188.6
(3)
P 		145.7
(0.8)
P 		107.8
(-1.2)
P 		73.8
(-3)
P 		91.3
(-2.1)
P 		116.7
(-0.8)
P 		115
C 167.2
(3)
P 		144.7
(1.7)
P 		84.4
(-1.9)
P 		71
(-2.6)
P 		65
(-3)
P 		92.8
(-1.4)
P 		102
avr 152.1
(3) 		145.2
(2.5) 		97.6
(-1.2) 		75.1
(-3) 		75.1
(-3) 		92.7
(-1.6) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181677_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4779
Sequence Type Exemplar sequence
Representative Public ID F40H3.4 NCBI
Target Description F40H3.4 /REP_DB=WormBase Gene ID /WP=CE19865 /TR=Q9TZK1 /GB=AAC67430.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062834(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062834 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-8) (fkh-8) mRNA, complete cds. 11/11 None
F40H3.4 ENSEMBL cdna:known chromosome:CEL140:II:6189545:6191327:-1 gene:F40H3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6189661-6191324(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fkh-8
Ensembl F40H3.4
Entrez Gene 174026 Entrez gene
SwissProt Q9TZK1 EMBL-EBI
WormBase CE19865 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC67430 Forkhead transcription factor family protein 8 [Caenorhabditis elegans] ref|NP_495235.1| ForKHead transcription factor family member (fkh-8) [Caenorhabditis elegans] 0.0
blast CAE59259 Hypothetical protein CBG02587 [Caenorhabditis briggsae] 1.0E-122
blast AAH14182 FOXJ3 protein [Homo sapiens] 1.0E-20
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.0000001089808E-27
Pfam IPR001766 EMBL-EBI Fork head transcription factor 1.3E-30
Sequence
Target Sequence 
>C. ELEGANS:181677_AT
gaattccattcgccataatctgtcattaaataaacaattcagccgcattgagaagactga
tggagatagaagaggatggtgggtttgtgtagatccgccagccaagaaaccacgaattct
caaaggatccccagtgcgagtaaatccaatttacgagcatctatatcataataaacaaga
catgccaaatttcactcctccgccagaagatccattcctgtcagaaattaatggaaatgt
acatgaacttacggaacacgaaataagagatttgaatttgttcgagtcatatgatctaaa
ctcatcattccgtgatgtctacaatcaaatttttgagaagtccacttcccccaatgggaa
gaagcaagctgctcaaatcgattggttgaaaataagcttagaagctgctggacttgatta
tcacgatgaacaagaacttcaagaagttgatacggataagttgaaagattacgtctacaa
tggattcccagcagaatgcgacagtgatgtctcaactccaagaacagactctggccgtca
ttcatcctcagattctgttctttcagccactcttcaaccagttaacaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCCATTCGCCATAATCTGTCA 76 325 318 Antisense
TGGTGGGTTTGTGTAGATCCGCCAG 532 553 394 Antisense
GAATTCTCAAAGGATCCCCAGTGCG 678 323 431 Antisense
CAAGACATGCCAAATTTCACTCCTC 696 183 493 Antisense
TCCGCCAGAAGATCCATTCCTGTCA 467 595 516 Antisense
AGCTGCTGGACTTGATTATCACGAT 278 81 720 Antisense
GACAGTGATGTCTCAACTCCAAGAA 351 369 817 Antisense
AAGAACAGACTCTGGCCGTCATTCA 380 151 837 Antisense
CGTCATTCATCCTCAGATTCTGTTC 153 247 853 Antisense
CTCAGATTCTGTTCTTTCAGCCACT 217 229 864 Antisense
GCCACTCTTCAACCAGTTAACAATG 656 277 883 Antisense
Sequence Source Affymetrix Proprietary Database