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[181676_at]

Description B0281.5 /REP_DB=WormBase Gene ID /WP=CE07716 /TR=O16612 /GB=AAB66084.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 68      4grp_total > 04      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 4.8
(-2.5)
A 		2.3
(-3)
A 		5.7
(-2.3)
A 		31
(3)
A 		29
B 22.2
(0.7)
A 		4.7
(-3)
A 		13.6
(-1.1)
A 		33.1
(3)
A 		28
C 14.7
(-1.8)
A 		5.7
(-2.7)
A 		2.8
(-3)
A 		63.9
(3)
A 		61
avr 13.9
(-1.5) 		4.2
(-3) 		7.4
(-2.5) 		42.7
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 246
1 2 3 4 5 6 range
A 34.7
(-2.1)
A 		5.9
(-2.9)
A 		2.2
(-3)
A 		5
(-2.9)
A 		48.1
(-1.8)
P 		229.7
(3)
P 		228
B 20.4
(-2.5)
A 		33.6
(-2)
A 		4.3
(-3)
A 		5
(-3)
A 		10.8
(-2.8)
A 		180.6
(3)
P 		176
C 23
(-2.6)
A 		7.3
(-3)
A 		10
(-2.9)
A 		19.5
(-2.7)
A 		8.8
(-3)
A 		248.3
(3)
P 		241
avr 26
(-2.4) 		15.6
(-2.7) 		5.5
(-3) 		9.8
(-2.9) 		22.6
(-2.5) 		219.5
(3) 		214
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 1.8
(-1.1)
A 		0.5
(-3)
A 		1.5
(-1.5)
A 		4.5
(3)
A 		4
B 7.1
(3)
A 		1.2
(-3)
A 		3.1
(-1.1)
A 		7.1
(3)
A 		6
C 4.9
(-0.6)
A 		1.6
(-2.6)
A 		1
(-3)
A 		10.8
(3)
A 		10
avr 4.6
(0.3) 		1.1
(-3) 		1.9
(-2.3) 		7.5
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 6.4
(-2.4)
A 		2.1
(-2.8)
A 		0.5
(-3)
A 		1.3
(-2.9)
A 		14.5
(-1.5)
P 		55.9
(3)
P 		55
B 7.1
(-2.4)
A 		11.2
(-2.1)
A 		1
(-3)
A 		1.3
(-3)
A 		4.4
(-2.7)
A 		67.4
(3)
P 		66
C 8.5
(-2.5)
A 		2.2
(-3)
A 		2.6
(-3)
A 		3.9
(-2.9)
A 		2.8
(-3)
A 		83.1
(3)
P 		81
avr 7.3
(-2.5) 		5.2
(-2.7) 		1.4
(-3) 		2.2
(-2.9) 		7.2
(-2.5) 		68.8
(3) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181676_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5290
Sequence Type Exemplar sequence
Representative Public ID B0281.5 NCBI
Target Description B0281.5 /REP_DB=WormBase Gene ID /WP=CE07716 /TR=O16612 /GB=AAB66084.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061829(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061829 NCBI Caenorhabditis elegans B0281.5 (B0281.5) mRNA, complete cds. 11/11 None
SNAP00000033214 ENSEMBL cdna:SNAP chromosome:CEL140:II:2297939:2300819:-1 11/11 None
GENEFINDER00000033223 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2297939:2300819:-1 11/11 None
B0281.5 ENSEMBL cdna:known chromosome:CEL140:II:2299943:2300819:-1 gene:B0281.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2299941-2300818(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0281.5
Entrez Gene 181899 Entrez gene
SwissProt O16612 EMBL-EBI
WormBase CE07716 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66084 Hypothetical protein B0281.5 [Caenorhabditis elegans] ref|NP_494230.1| B0281.5 [Caenorhabditis elegans] 1.0E-113
blast AAB66084 Hypothetical protein B0281.5 [Caenorhabditis elegans] ref|NP_494230.1| B0281.5 [Caenorhabditis elegans] 1.0E-112
blast AAB66084 Hypothetical protein B0281.5 [Caenorhabditis elegans] ref|NP_494230.1| B0281.5 [Caenorhabditis elegans] 1.0E-109
blast AAB66083 Hypothetical protein B0281.4 [Caenorhabditis elegans] ref|NP_494229.2| B0281.4 [Caenorhabditis elegans] 2.0E-65
blast AAB66083 Hypothetical protein B0281.4 [Caenorhabditis elegans] ref|NP_494229.2| B0281.4 [Caenorhabditis elegans] 9.0E-60
blast AAC68762 Hypothetical protein C40A11.7 [Caenorhabditis elegans] ref|NP_494199.1| C40A11.7 [Caenorhabditis elegans] 7.0E-44
blast AAC68757 Hypothetical protein C40A11.6 [Caenorhabditis elegans] ref|NP_494197.1| C40A11.6 [Caenorhabditis elegans] 7.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.6E-46
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 6.5E-13
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.1E-13
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 6.5E-13
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 2.1E-5
Sequence
Target Sequence 
>C. ELEGANS:181676_AT
aggacgattccaactgtttcttcattgatcggtctccgaagcactttgaaacagttctca
actacatgagatccggtgatgtagtccttccggactctaaaaaagacatgctagaattga
aaaaagaagctgaatactacctgctgagcaatttggcaaaattatgtcaaccaccaacgg
acaactttaaaacatgcacacctgaggaactgatgcgtgtagttgcgaacacggataaga
aagttattatgatcaactatttaacttacaatggtgagctcgtcttctacccgtggggtt
tcaatcttgttcattttttaattcaaaatcgcgattatgtggaggatgtctactttcgaa
gaacagaatctcaatacccagaggacggaccttttccaaatgagaagccatattggactt
ttcttgtctacaattctacgcgttcaggtcgtcgaccattccggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGACGATTCCAACTGTTTCTTCAT 263 55 134 Antisense
ACTGTTTCTTCATTGATCGGTCTCC 696 97 146 Antisense
ATCGGTCTCCGAAGCACTTTGAAAC 558 35 161 Antisense
GAGATCCGGTGATGTAGTCCTTCCG 302 395 201 Antisense
GCTGAATACTACCTGCTGAGCAATT 527 293 262 Antisense
TTACAATGGTGAGCTCGTCTTCTAC 315 683 399 Antisense
CCCGTGGGGTTTCAATCTTGTTCAT 164 259 423 Antisense
CCAGAGGACGGACCTTTTCCAAATG 220 273 511 Antisense
GCCATATTGGACTTTTCTTGTCTAC 239 281 540 Antisense
GTCTACAATTCTACGCGTTCAGGTC 102 467 559 Antisense
CGTTCAGGTCGTCGACCATTCCGGC 565 247 574 Antisense
Sequence Source Affymetrix Proprietary Database