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[181668_at]

Description F25F8.1 /REP_DB=WormBase Gene ID /WP=CE09635 /TR=P91272 /GB=AAB42251.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 60      6grp_total > 13      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 431
1 2 3 4 range
A 434.9
(3)
P 		330.3
(0.9)
P 		274.3
(-0.2)
P 		137.8
(-3)
P 		297
B 458.4
(3)
P 		315.3
(0.8)
P 		264
(0)
P 		69.2
(-3)
P 		389
C 500.4
(3)
P 		321.2
(0.2)
P 		231.5
(-1.1)
P 		110.4
(-3)
P 		390
avr 464.6
(3) 		322.3
(0.6) 		256.6
(-0.5) 		105.8
(-3) 		359

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 503.4
(3)
P 		495.8
(2.9)
P 		424.2
(1.6)
P 		331.1
(0.1)
P 		201.1
(-2.2)
P 		152.6
(-3)
P 		351
B 503.2
(2.6)
P 		530.1
(3)
P 		348.3
(-0)
P 		352.2
(0)
P 		238
(-1.9)
P 		168.9
(-3)
P 		361
C 421.8
(0.8)
P 		574.7
(3)
P 		376.9
(0.2)
P 		344.6
(-0.3)
P 		177.3
(-2.6)
P 		151.1
(-3)
P 		424
avr 476.1
(2.1) 		533.5
(3) 		383.1
(0.6) 		342.6
(-0) 		205.5
(-2.2) 		157.5
(-3) 		376
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 158.4
(3)
P 		71.7
(-0.8)
P 		71.7
(-0.8)
P 		20.2
(-3)
P 		138
B 146.2
(3)
P 		77.9
(-0.1)
P 		61.2
(-0.9)
P 		14.7
(-3)
P 		132
C 165.6
(3)
P 		92.4
(0)
P 		80.7
(-0.5)
P 		18.6
(-3)
P 		147
avr 156.7
(3) 		80.7
(-0.3) 		71.2
(-0.7) 		17.8
(-3) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 92.8
(-0.6)
P 		176.3
(3)
P 		92.4
(-0.6)
P 		84
(-1)
P 		60.7
(-2)
P 		37.1
(-3)
P 		139
B 175.1
(2.9)
P 		176.5
(3)
P 		84.6
(-1.9)
P 		93.3
(-1.4)
P 		96.7
(-1.2)
P 		63
(-3)
P 		114
C 156.3
(2.1)
P 		175.4
(3)
P 		95.9
(-0.8)
P 		69.3
(-2.1)
P 		57.4
(-2.7)
P 		50.6
(-3)
P 		125
avr 141.4
(1.3) 		176.1
(3) 		91
(-1.1) 		82.2
(-1.5) 		71.6
(-2) 		50.2
(-3) 		126
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181668_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1772
Sequence Type Exemplar sequence
Representative Public ID F25F8.1 NCBI
Target Description F25F8.1 /REP_DB=WormBase Gene ID /WP=CE09635 /TR=P91272 /GB=AAB42251.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059068(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059068 NCBI Caenorhabditis elegans F25F8.1 (F25F8.1) mRNA, complete cds. 11/11 None
GENEFINDER00000002869 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4891249:4895327:1 11/11 None
SNAP00000002867 ENSEMBL cdna:SNAP chromosome:CEL140:I:4891736:4895327:1 11/11 None
F25F8.1 ENSEMBL cdna:known chromosome:CEL140:I:4884904:4895496:1 gene:F25F8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4884913-4895327(+) 97.7 100.0
Public Domain and Genome References
Gene Title
Ensembl F25F8.1
Entrez Gene 172104 Entrez gene
SwissProt P91272 EMBL-EBI
WormBase CE32636 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42251 Hypothetical protein F25F8.1 [Caenorhabditis elegans] ref|NP_491469.2| F25F8.1 [Caenorhabditis elegans] 0.0
blast CAE73121 Hypothetical protein CBG20503 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:181668_AT
gcaggacccggtggattactgtaggaccgagaggtcacctatttatgacaagtggagaca
atgtgaagagtgcgttatggacttatattcgaagcaagaaagcttggaaattattgaaag
aatcgagaaaatctagatatcaatatttaactgttgccgaggatcaagctgattataaag
cagtagtattattaacatgtgatgctgccaacaatcgtcttctcctttttgtgattgatc
attcaatgagcttaatcaatgaatatgatttgagcaaaacatatcggctcaatgagcata
tccagtcacctgcatctgcaattgttgataagaatggaaatttactaatccttgactacg
ctagtggaagactttggattcttctgtccggtgtaaaaggggtccgccgcctgaaacaag
tgcctttgccagacatcgtgagaccccaagaggctcttggaatctgtactagaggtgatg
ttctctacttggctttattcaatcgtcgtgaaatactctgtacacgatatttggacgatg
gagtatttaaaccaacagctgcaactggagtctcatcttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGGACCCGGTGGATTACTGTAGG 432 311 1520 Antisense
ATGTGATGCTGCCAACAATCGTCTT 664 49 1716 Antisense
TCGTCTTCTCCTTTTTGTGATTGAT 337 605 1734 Antisense
GGCTCAATGAGCATATCCAGTCACC 341 539 1805 Antisense
TCCAGTCACCTGCATCTGCAATTGT 65 591 1820 Antisense
AATTTACTAATCCTTGACTACGCTA 87 175 1858 Antisense
AGACTTTGGATTCTTCTGTCCGGTG 631 69 1888 Antisense
AAACAAGTGCCTTTGCCAGACATCG 337 129 1933 Antisense
GACATCGTGAGACCCCAAGAGGCTC 114 371 1951 Antisense
GTGATGTTCTCTACTTGGCTTTATT 706 483 1994 Antisense
AGCTGCAACTGGAGTCTCATCTTCT 306 81 2076 Antisense
Sequence Source Affymetrix Proprietary Database