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[181667_s_at]

Description F58A6.5 /REP_DB=WormBase Gene ID /WP=CE04678 /TR=Q20960 /GB=AAA96200.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 27      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 24.6
(-2.5)
A 		82.5
(3)
A 		19.6
(-3)
A 		77.6
(2.5)
A 		63
B 58.7
(0.9)
A 		74.7
(3)
A 		43.8
(-1)
A 		28.7
(-3)
A 		46
C 49.4
(-0.2)
A 		48
(-0.2)
A 		6
(-3)
A 		97.4
(3)
A 		91
avr 44.2
(-0.2) 		68.4
(3) 		23.1
(-3) 		67.9
(2.9) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 20.6
(-2.7)
A 		8.2
(-3)
A 		11.7
(-2.9)
A 		67.2
(-1.7)
A 		175
(0.6)
P 		288.7
(3)
P 		281
B 55.5
(-2.5)
A 		42.1
(-2.8)
A 		33.2
(-3)
A 		46.5
(-2.7)
A 		175.3
(0.2)
P 		297.6
(3)
P 		264
C 31.2
(-2.5)
A 		11.2
(-3)
A 		20.4
(-2.8)
A 		75.6
(-1.3)
A 		170.2
(1.2)
P 		236.3
(3)
P 		225
avr 35.8
(-2.6) 		20.5
(-3) 		21.8
(-3) 		63.1
(-2) 		173.5
(0.6) 		274.2
(3) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 9
(-1.2)
A 		17.9
(3)
A 		5.1
(-3)
A 		11.4
(-0)
A 		13
B 18.7
(3)
A 		18.5
(2.9)
A 		10.2
(-1)
A 		6.1
(-3)
A 		13
C 16.3
(3)
A 		13.8
(1.9)
A 		2.1
(-3)
A 		16.4
(3)
A 		14
avr 14.7
(1.9) 		16.7
(3) 		5.8
(-3) 		11.3
(0) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 3.8
(-2.9)
A 		2.9
(-3)
A 		2.6
(-3)
A 		17.1
(-1.7)
A 		52.8
(1.4)
P 		70.3
(3)
P 		68
B 19.3
(-2.3)
A 		14
(-2.7)
A 		8.1
(-3)
A 		12.3
(-2.8)
A 		71.2
(0.7)
P 		111
(3)
P 		103
C 11.6
(-2.4)
A 		3.4
(-3)
A 		5.2
(-2.9)
A 		15.2
(-2.1)
A 		55.1
(1.1)
P 		79.1
(3)
P 		76
avr 11.6
(-2.5) 		6.8
(-2.9) 		5.3
(-3) 		14.9
(-2.3) 		59.7
(1) 		86.8
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181667_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5250
Sequence Type Exemplar sequence
Representative Public ID F58A6.5 NCBI
Target Description F58A6.5 /REP_DB=WormBase Gene ID /WP=CE04678 /TR=Q20960 /GB=AAA96200.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062554(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062554 NCBI Caenorhabditis elegans F58A6.5 (F58A6.5) mRNA, complete cds. 11/11 None
SNAP00000019947 ENSEMBL cdna:SNAP chromosome:CEL140:II:5146979:5148397:-1 11/11 None
GENEFINDER00000019955 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5146979:5148397:-1 11/11 None
F58A6.5 ENSEMBL cdna:known chromosome:CEL140:II:5146979:5148443:-1 gene:F58A6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5146976-5148395(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58A6.5
Entrez Gene 173883 Entrez gene
SwissProt Q20960 EMBL-EBI
WormBase CE04678 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA96200 Hypothetical protein F58A6.5 [Caenorhabditis elegans] ref|NP_494955.1| F58A6.5 [Caenorhabditis elegans] 1.0E-168
blast CAE59020 Hypothetical protein CBG02297 [Caenorhabditis briggsae] 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494955.1 7 102-124,129-151,164-181,191-210,217-239,243-262,275-292
F58A6.5 7 102-124,129-151,164-181,191-210,217-239,243-262,275-292
SNAP00000019947 7 102-124,129-151,164-181,191-210,217-239,243-262,275-292
GENEFINDER00000019955 7 102-124,129-151,164-181,191-210,217-239,243-262,275-292
Sequence
Target Sequence 
>C. ELEGANS:181667_S_AT
tcgaagactttcgcaaacgccacatttcttctaattgttgcctccatctcatcattcatt
ggttcatatatcactgctccagtctttggaatcattttccttaaccttggagcaattctc
gtcatcactattgtaatccctctcattgccagttatgtgtattacaacttgagagtccga
tcattggcagaaaaacgaattgcaagctgtgcattcatttttgttcagtcagtactaatt
gggttcataaatcaggatgattgggttgaatctgctccgtttacagtttttacacaaatg
attgcatcatttgtgtatccaatggtattgattcatactgacaacagacagaaaatcctt
ggttctgttgctggattatcgattttttgtcatttgttcatcggactcatcttttctgga
atcaatggaccttttttgatgatggctgtcatgtatactgttcttgccgtggctttaatt
caatattctattgcttttcggactcagactgactatgatatgatgcacttggctatgcaa
tacgtatttctcgtttctggtgtcaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGAAGACTTTCGCAAACGCCACAT 381 601 307 Antisense
ACATTTCTTCTAATTGTTGCCTCCA 494 105 328 Antisense
TGGTTCATATATCACTGCTCCAGTC 334 551 366 Antisense
GAGCAATTCTCGTCATCACTATTGT 282 391 416 Antisense
ATCCCTCTCATTGCCAGTTATGTGT 624 37 442 Antisense
GGTTGAATCTGCTCCGTTTACAGTT 666 505 570 Antisense
GAAAATCCTTGGTTCTGTTGCTGGA 629 353 657 Antisense
TCATCGGACTCATCTTTTCTGGAAT 305 621 704 Antisense
GTCATGTATACTGTTCTTGCCGTGG 109 467 754 Antisense
ATTGCTTTTCGGACTCAGACTGACT 435 5 796 Antisense
GTATTTCTCGTTTCTGGTGTCAAAA 688 451 850 Antisense
Sequence Source Affymetrix Proprietary Database