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[181659_at]

Description F35D2.2 /REP_DB=WormBase Gene ID /WP=CE02724 /TR=Q20044 /GB=AAA68326.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 49      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 226
1 2 3 4 range
A 50.9
(-3)
A 		186.3
(2.3)
P 		204
(3)
P 		60.8
(-2.6)
A 		153
B 53.4
(-2.3)
P 		253.1
(3)
P 		159.6
(0.5)
P 		27.3
(-3)
A 		226
C 51.1
(-3)
M 		185.1
(1.2)
P 		242.1
(3)
P 		83.7
(-2)
A 		191
avr 51.8
(-3) 		208.2
(3) 		201.9
(2.8) 		57.3
(-2.8) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 58.2
(-1.3)
P 		161.7
(3)
P 		124.3
(1.4)
P 		130.1
(1.7)
P 		110.3
(0.8)
P 		18.9
(-3)
A 		143
B 56.8
(-2.7)
A 		152.4
(3)
P 		127.3
(1.5)
P 		106.9
(0.3)
P 		72.8
(-1.7)
P 		51.5
(-3)
A 		101
C 32.3
(-3)
A 		189.2
(3)
P 		125.1
(0.5)
P 		159
(1.8)
P 		70.8
(-1.5)
P 		51.2
(-2.3)
A 		157
avr 49.1
(-2.6) 		167.8
(3) 		125.6
(1) 		132
(1.3) 		84.6
(-0.9) 		40.5
(-3) 		127
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 18.5
(-1.7)
A 		40.4
(1.3)
P 		53.3
(3)
P 		8.9
(-3)
A 		44
B 17
(-1.8)
P 		62.5
(3)
P 		37
(0.3)
P 		5.8
(-3)
A 		57
C 16.9
(-2.8)
M 		53.2
(0.3)
P 		84.4
(3)
P 		14.1
(-3)
A 		70
avr 17.5
(-2) 		52
(2.2) 		58.2
(3) 		9.6
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 10.7
(-2.3)
P 		57.5
(3)
P 		27.1
(-0.4)
P 		33
(0.2)
P 		33.3
(0.3)
P 		4.6
(-3)
A 		53
B 19.8
(-2.9)
A 		50.7
(3)
P 		30.9
(-0.8)
P 		28.3
(-1.3)
P 		29.6
(-1)
P 		19.2
(-3)
A 		32
C 12
(-3)
A 		57.7
(3)
P 		31.8
(-0.4)
P 		32
(-0.4)
P 		22.9
(-1.6)
P 		17.1
(-2.3)
A 		46
avr 14.2
(-2.9) 		55.3
(3) 		29.9
(-0.7) 		31.1
(-0.5) 		28.6
(-0.8) 		13.6
(-3) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181659_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5214
Sequence Type Exemplar sequence
Representative Public ID F35D2.2 NCBI
Target Description F35D2.2 /REP_DB=WormBase Gene ID /WP=CE02724 /TR=Q20044 /GB=AAA68326.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063188(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063188 NCBI Caenorhabditis elegans F35D2.2 (F35D2.2) mRNA, complete cds. 11/11 None
SNAP00000006813 ENSEMBL cdna:SNAP chromosome:CEL140:II:7574373:7575699:1 11/11 None
GENEFINDER00000006823 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7574373:7580166:1 11/11 None
F35D2.2 ENSEMBL cdna:known chromosome:CEL140:II:7574373:7575699:1 gene:F35D2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7573944-7575696(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35D2.2
Entrez Gene 185277 Entrez gene
SwissProt Q20044 EMBL-EBI
WormBase CE36839 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68326 Hypothetical protein F35D2.2 [Caenorhabditis elegans] ref|NP_495589.2| F35D2.2 [Caenorhabditis elegans] 0.0
blast CAE67492 Hypothetical protein CBG13000 [Caenorhabditis briggsae] 1.0E-177
blast CAE67492 Hypothetical protein CBG13000 [Caenorhabditis briggsae] 1.0E-170
blast AAA68327 Hypothetical protein F35D2.1 [Caenorhabditis elegans] ref|NP_495590.1| F35D2.1 [Caenorhabditis elegans] 6.0E-80
blast CAE67493 Hypothetical protein CBG13001 [Caenorhabditis briggsae] 5.0E-51
Sequence
Target Sequence 
>C. ELEGANS:181659_AT
aatagaaatgcccatccaacacttccacttggaaagtttgcaaaaacatatgttccactc
aattgtaagcctccattatgcaatccatatcaaatgaacttcggacttggtgttgagcat
gattggggaggctctgatggagttgaaggagatattgatgttccaattcccatttctaaa
ggaatggcttacagaatgccattctctggaaaagtttactacaatcgtgaaaatgtcaca
gttacatatggtcaaaatctgggtcctatcgagccatattccagtttgttcgattatcaa
aaacacagagatcctgcattgcgtatccctagaaggtggacaagaagtgttcaagaaccg
gttgaaatacaaaagccgatgatgagaatgagaaatatggttgtaccaggaatggcatat
atgccttatatgaattcagtttctctatacccatatatttcgaaaatgcctgagcaatgg
cttcagcctgatccatattattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATAGAAATGCCCATCCAACACTTC 137 173 613 Antisense
TGTTCCACTCAATTGTAAGCCTCCA 617 561 663 Antisense
AAGCCTCCATTATGCAATCCATATC 261 149 679 Antisense
GAACTTCGGACTTGGTGTTGAGCAT 101 349 708 Antisense
GATATTGATGTTCCAATTCCCATTT 159 427 763 Antisense
AAATCTGGGTCCTATCGAGCCATAT 618 115 867 Antisense
GAGCCATATTCCAGTTTGTTCGATT 675 381 883 Antisense
CAGAGATCCTGCATTGCGTATCCCT 564 205 918 Antisense
GAATTCAGTTTCTCTATACCCATAT 448 323 1044 Antisense
ATGCCTGAGCAATGGCTTCAGCCTG 499 41 1078 Antisense
GGCTTCAGCCTGATCCATATTATTC 478 537 1091 Antisense
Sequence Source Affymetrix Proprietary Database