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[181655_at]

Description F58A6.4 /REP_DB=WormBase Gene ID /WP=CE04677 /TR=Q20958 /GB=AAA96198.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 41      6grp_cgc > 74      6grp_cgc_sub > 02_03_04_05      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 73.3
(-3)
A 		211.2
(2.3)
A 		179.4
(1.1)
A 		228.2
(3)
A 		155
B 86.5
(-3)
A 		185.3
(0.8)
A 		241.1
(3)
A 		164.4
(0)
A 		155
C 106.7
(-3)
A 		243.2
(3)
P 		184.7
(0.4)
P 		143.9
(-1.4)
A 		137
avr 88.8
(-3) 		213.2
(3) 		201.7
(2.4) 		178.8
(1.3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 17.2
(-3)
A 		111.1
(2.2)
A 		108.4
(2)
A 		125.9
(3)
A 		81.2
(0.5)
A 		63.2
(-0.5)
A 		109
B 32.4
(-2.7)
A 		174.4
(3)
A 		140.8
(1.6)
A 		146.7
(1.9)
A 		107.8
(0.3)
A 		26.1
(-3)
A 		148
C 34
(-3)
A 		189.8
(3)
A 		127.4
(0.6)
A 		117.1
(0.2)
A 		80.2
(-1.2)
A 		48.1
(-2.5)
A 		156
avr 27.9
(-3) 		158.4
(3) 		125.5
(1.5) 		129.9
(1.7) 		89.7
(-0.2) 		45.8
(-2.2) 		131
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 26.7
(-3)
A 		45.9
(2.7)
A 		46.9
(3)
A 		33.4
(-1)
A 		20
B 27.6
(-3)
A 		45.8
(0.9)
A 		55.9
(3)
A 		35
(-1.4)
A 		28
C 35.3
(-1.6)
A 		69.9
(3)
P 		64.4
(2.3)
P 		24.3
(-3)
A 		46
avr 29.9
(-3) 		53.9
(2.6) 		55.7
(3) 		30.9
(-2.8) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 3.2
(-3)
A 		39.5
(3)
A 		23.6
(0.4)
A 		31.9
(1.7)
A 		24.5
(0.5)
A 		15.4
(-1)
A 		36
B 11.3
(-2.8)
A 		58
(3)
A 		34.2
(0)
A 		38.9
(0.6)
A 		43.8
(1.2)
A 		9.7
(-3)
A 		48
C 12.6
(-3)
A 		57.9
(3)
A 		32.4
(-0.4)
A 		23.6
(-1.5)
A 		26
(-1.2)
A 		16.1
(-2.5)
A 		45
avr 9
(-3) 		51.8
(3) 		30.1
(-0) 		31.5
(0.2) 		31.4
(0.1) 		13.7
(-2.3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181655_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4877
Sequence Type Exemplar sequence
Representative Public ID F58A6.4 NCBI
Target Description F58A6.4 /REP_DB=WormBase Gene ID /WP=CE04677 /TR=Q20958 /GB=AAA96198.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062552(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062552 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-29) (nas-29) mRNA, complete cds. 11/11 None
SNAP00000019945 ENSEMBL cdna:SNAP chromosome:CEL140:II:5138678:5141791:-1 10/11 None
GENEFINDER00000019957 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5138678:5141791:-1 11/11 None
F58A6.4 ENSEMBL cdna:known chromosome:CEL140:II:5138678:5141009:-1 gene:F58A6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5138675-5141007(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nas-29
Ensembl F58A6.4
Entrez Gene 186488 Entrez gene
SwissProt Q20958 EMBL-EBI
WormBase CE04677 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA96198 Nematode astacin protease protein 29 [Caenorhabditis elegans] ref|NP_494953.1| Nematode AStacin protease family member (nas-29) [Caenorhabditis elegans] 0.0
blast Q20958 Zinc metalloproteinase nas-29 precursor (Nematode astacin 29) 0.0
blast CAE59050 Hypothetical protein CBG02333 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 4.3E-106
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 4.3E-106
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000019945 1 7-26
GENEFINDER00000019957 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:181655_AT
gaaaagtgtccaactcaagctccttgccaaaatggtggcttcactaattcccgtaactgc
aaagtttgcaaatgtccaactggctttggaggggcttactgtcaactcattgcttcatca
ttttccccattctgtggtggctacctaaacgcggaagaaacaactcgaagatttgatatc
actattcgccaatcgacaactacaagatcgaaaacatgtgtttatcacataaaagctcca
gagggaaaacggattataattgacattctgaaaatagactcgaagtgtattgaaggatgc
tggcaagatggattagagttgaagatgaaaaaggattttaggcccgttgggtatagattt
tgctgccctgagtcatctcgtcggaaagtgatatcagaagggaacatggtcccttttatg
gttttctcaaaagaacatgatttttcggtttcatttgagtactcttttgtttccaccagc
gcaggatttgacgacgaaaagaatgattcagatgttatcgttgacaatttggatggagtt
tttgtgtccgacacttccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAAGTGTCCAACTCAAGCTCCTT 68 355 739 Antisense
AATGGTGGCTTCACTAATTCCCGTA 193 159 769 Antisense
GAGGGGCTTACTGTCAACTCATTGC 348 401 827 Antisense
CAACTCATTGCTTCATCATTTTCCC 544 187 841 Antisense
CCCCATTCTGTGGTGGCTACCTAAA 388 261 863 Antisense
TTGATATCACTATTCGCCAATCGAC 671 703 911 Antisense
TAGATTTTGCTGCCCTGAGTCATCT 682 651 1092 Antisense
CATGGTCCCTTTTATGGTTTTCTCA 696 207 1143 Antisense
TTTGAGTACTCTTTTGTTTCCACCA 193 667 1192 Antisense
GTTTCCACCAGCGCAGGATTTGACG 172 451 1207 Antisense
GAGTTTTTGTGTCCGACACTTCCCT 351 399 1274 Antisense
Sequence Source Affymetrix Proprietary Database