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[181653_at]

Description T22H9.3 /REP_DB=WormBase Gene ID /WP=CE20101 /TR=Q9TXN7 /GB=AAC69228.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 68      4grp_total > 26      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 18
(-3)
A 		39.2
(-0.8)
A 		66.4
(1.9)
A 		77.1
(3)
A 		59
B 9.9
(-3)
A 		21
(-0.6)
A 		14.7
(-1.9)
A 		37.3
(3)
A 		27
C 28.9
(-0.5)
A 		58.8
(3)
A 		7.9
(-3)
A 		38.3
(0.6)
A 		51
avr 18.9
(-3) 		39.7
(0.9) 		29.7
(-1) 		50.9
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 41.4
(-0.9)
A 		32.5
(-1.4)
A 		13.3
(-2.5)
A 		5.1
(-3)
A 		32.1
(-1.5)
A 		110.8
(3)
A 		106
B 41.4
(0.8)
A 		6.3
(-3)
A 		61.8
(3)
A 		16
(-2)
A 		27.2
(-0.7)
A 		54.1
(2.2)
A 		56
C 21.4
(-2.2)
A 		11.4
(-3)
A 		12.3
(-2.9)
A 		42.7
(-0.6)
A 		29.7
(-1.6)
A 		89.5
(3)
A 		78
avr 34.7
(-1.4) 		16.7
(-3) 		29.1
(-1.9) 		21.3
(-2.6) 		29.7
(-1.9) 		84.8
(3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 6.6
(-3)
A 		8.5
(-1.9)
A 		17.4
(3)
A 		11.3
(-0.4)
A 		11
B 3.2
(-3)
A 		5.2
(-0.5)
A 		3.4
(-2.8)
A 		8
(3)
A 		5
C 9.6
(-0.1)
A 		16.9
(3)
A 		2.8
(-3)
A 		6.5
(-1.4)
A 		14
avr 6.5
(-3) 		10.2
(3) 		7.9
(-0.7) 		8.6
(0.4) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 7.6
(-1.5)
A 		11.6
(-0.6)
A 		2.9
(-2.6)
A 		1.3
(-3)
A 		9.7
(-1)
A 		27
(3)
A 		26
B 14.4
(1.1)
A 		2.1
(-3)
A 		15
(1.3)
A 		4.2
(-2.3)
A 		11.1
(-0)
A 		20.2
(3)
A 		18
C 7.9
(-1.9)
A 		3.5
(-2.9)
A 		3.1
(-3)
A 		8.6
(-1.8)
A 		9.6
(-1.6)
A 		30
(3)
A 		27
avr 10
(-1.5) 		5.7
(-2.7) 		7
(-2.3) 		4.7
(-3) 		10.1
(-1.5) 		25.7
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181653_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15651
Sequence Type Exemplar sequence
Representative Public ID T22H9.3 NCBI
Target Description T22H9.3 /REP_DB=WormBase Gene ID /WP=CE20101 /TR=Q9TXN7 /GB=AAC69228.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070776(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070776 NCBI Caenorhabditis elegans T22H9.3 (T22H9.3) mRNA, complete cds. 11/11 None
SNAP00000007600 ENSEMBL cdna:SNAP chromosome:CEL140:V:341432:358664:1 10/11 None
GENEFINDER00000007606 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:343315:347283:1 11/11 None
T22H9.3 ENSEMBL cdna:known chromosome:CEL140:V:343289:347452:1 gene:T22H9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:343314-347283(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22H9.3
Entrez Gene 178560 Entrez gene
SwissProt Q9TXN7 EMBL-EBI
WormBase CE20101 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69228 Hypothetical protein T22H9.3 [Caenorhabditis elegans] ref|NP_503177.1| T22H9.3 [Caenorhabditis elegans] 0.0
blast CAA86741 Hypothetical protein C16C10.3 [Caenorhabditis elegans] ref|NP_497834.1| C16C10.3 [Caenorhabditis elegans] sp|Q09249|YQ53_CAEEL Hypothetical protein C16C10.3 in chromosome III 0.0
blast CAE58274 Hypothetical protein CBG01380 [Caenorhabditis briggsae] 0.0
blast CAJ30198 autophagy protein 9 [Caenorhabditis elegans] 0.0
blast CAE58273 Hypothetical protein CBG01379 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 1.3E-45
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 2.3E-117
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 1.3E-45
Pfam IPR007241 EMBL-EBI Autophagy protein Apg9 1.0E-126
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 2.3E-117
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000007600 5 1071-1093,1237-1259,1320-1342,1349-1371,1418-1440
Sequence
Target Sequence 
>C. ELEGANS:181653_AT
gctcgtcgtcatcattgctcagagagattctcacgttcgggtctttccggcacacataac
tgggtcaaaagctggacagcaaaatgtgacgtctggaacttgtgtggatagtgtgatcac
ttcgttgggccatcaggagttcattttgtcctcgcagatgccgctattgggaaccgtccg
tccttgcaagtacacaattctcaccaatgaccccaactggaccaagactgagatcacaca
tctcacttatttccgagcttttggccatcaagtatcctatcagccaccatcgttccctga
tgtgctctacgccgctgagaatctcgccaagagaggaagaaacaactacaaagttcatca
acgattcgttaatttgcaagctgtcgagcgtagtgtgattgcggagaattcggatcttgt
tagcgaggagatgcgcgagcaactcgccgctgcaattgtcgatgaaatgtccgctgcgat
ca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGTCGTCATCATTGCTCAGAGA 552 299 2466 Antisense
CAGAGAGATTCTCACGTTCGGGTCT 18 207 2485 Antisense
TTTCCGGCACACATAACTGGGTCAA 273 653 2509 Antisense
ATCACTTCGTTGGGCCATCAGGAGT 489 27 2581 Antisense
AGGAGTTCATTTTGTCCTCGCAGAT 205 57 2600 Antisense
GCAGATGCCGCTATTGGGAACCGTC 507 311 2619 Antisense
GTACACAATTCTCACCAATGACCCC 675 459 2655 Antisense
AGATCACACATCTCACTTATTTCCG 186 67 2696 Antisense
TTATTTCCGAGCTTTTGGCCATCAA 530 677 2712 Antisense
ACTCGCCGCTGCAATTGTCGATGAA 511 99 2907 Antisense
GTCGATGAAATGTCCGCTGCGATCA 407 473 2923 Antisense
Sequence Source Affymetrix Proprietary Database