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[181648_at]

Description T19F4.1 /REP_DB=WormBase Gene ID /WP=CE07523 /TR=Q22595 /GB=AAA98000.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      6grp_highest_nonorm > 90      6grp_total > 16      4grp_total > 29      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 90.5
(0.3)
P 		79.9
(-0.6)
P 		53.5
(-3)
A 		120.8
(3)
P 		67
B 116.3
(2.4)
P 		63.2
(-1.8)
P 		47.9
(-3)
A 		124
(3)
P 		76
C 85.3
(1.7)
P 		95.9
(3)
P 		46.9
(-3)
A 		70.2
(-0.1)
P 		49
avr 97.4
(2.2) 		79.7
(0.3) 		49.4
(-3) 		105
(3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 108.9
(3)
P 		86.2
(0.7)
P 		52.4
(-2.7)
P 		49.1
(-3)
P 		65.5
(-1.4)
P 		54.5
(-2.5)
P 		60
B 103.5
(3)
P 		85.6
(1.5)
P 		36.3
(-2.6)
P 		31.7
(-3)
P 		65
(-0.2)
P 		45.8
(-1.8)
P 		72
C 144.5
(3)
P 		74.8
(-1.2)
P 		61.5
(-2.1)
P 		47.1
(-2.9)
P 		45.9
(-3)
P 		65.4
(-1.8)
P 		99
avr 119
(3) 		82.2
(0.1) 		50.1
(-2.4) 		42.6
(-3) 		58.8
(-1.7) 		55.2
(-2) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 32.9
(3)
P 		17.3
(-2)
P 		14
(-3)
A 		17.7
(-1.8)
P 		19
B 37.1
(3)
P 		15.6
(-2)
P 		11.1
(-3)
A 		26.4
(0.5)
P 		26
C 28.3
(3)
P 		27.6
(2.7)
P 		16.4
(-1.3)
A 		11.8
(-3)
P 		17
avr 32.8
(3) 		20.2
(-1) 		13.8
(-3) 		18.6
(-1.5) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 20.1
(-0.3)
P 		30.7
(3)
P 		11.4
(-3)
P 		12.4
(-2.7)
P 		19.8
(-0.4)
P 		13.3
(-2.4)
P 		19
B 36
(3)
P 		28.5
(1.4)
P 		8.8
(-2.9)
P 		8.4
(-3)
P 		26.4
(0.9)
P 		17.1
(-1.1)
P 		28
C 53.5
(3)
P 		22.8
(-1.2)
P 		15.7
(-2.2)
P 		9.5
(-3)
P 		14.9
(-2.3)
P 		21.9
(-1.3)
P 		44
avr 36.5
(3) 		27.3
(0.9) 		12
(-2.6) 		10.1
(-3) 		20.4
(-0.7) 		17.4
(-1.3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181648_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12347
Sequence Type Exemplar sequence
Representative Public ID T19F4.1 NCBI
Target Description T19F4.1 /REP_DB=WormBase Gene ID /WP=CE07523 /TR=Q22595 /GB=AAA98000.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072345(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072345 NCBI Caenorhabditis elegans T19F4.1b (T19F4.1) mRNA, complete cds. 10/11 None
SNAP00000028426 ENSEMBL cdna:SNAP chromosome:CEL140:V:6872903:6876163:1 10/11 None
GENEFINDER00000028428 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6872903:6875619:1 11/11 None
T19F4.1b ENSEMBL cdna:known chromosome:CEL140:V:6872903:6876376:1 gene:T19F4.1 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_072346 8/11 Cross Hyb Matching Probes None
T19F4.1a 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179076 2 1 NM_072345 181648_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6872903-6875688(+) 100.0 100.0
Public Domain and Genome References
Ensembl T19F4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL02525 Hypothetical protein T19F4.1b [Caenorhabditis elegans] ref|NP_504746.1| T19F4.1b [Caenorhabditis elegans] 0.0
blast CAE71965 Hypothetical protein CBG19036 [Caenorhabditis briggsae] 0.0
blast T29683 hypothetical protein T19F4.1 - Caenorhabditis elegans 0.0
blast AAL02525 Hypothetical protein T19F4.1b [Caenorhabditis elegans] ref|NP_504746.1| T19F4.1b [Caenorhabditis elegans] 1.0E-161
blast T29683 hypothetical protein T19F4.1 - Caenorhabditis elegans 1.0E-158
blast AAL02524 Hypothetical protein T19F4.1a [Caenorhabditis elegans] ref|NP_504747.1| T19F4.1a [Caenorhabditis elegans] 1.0E-157
blast CAE71965 Hypothetical protein CBG19036 [Caenorhabditis briggsae] 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.3E-7
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.9E-7
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.3E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504746.1 7 10-29,41-63,78-100,130-147,182-204,225-247,267-289
T19F4.1b 7 10-29,41-63,78-100,130-147,182-204,225-247,267-289
SNAP00000028426 5 7-41,56-78,108-125,160-182,203-225
GENEFINDER00000028428 6 10-29,41-63,78-100,130-147,182-204,225-247
Sequence
Target Sequence 
>C. ELEGANS:181648_AT
ggtggtactacgttggctactacacaattctttacattgtcacacattttttggtgccat
tttctgttatggcatttgcaaatgggcatgtgatagttgcaatgtgcaaacttagcaaaa
cacggcagatgctcacaagacagagcacgcggggactggcatattggctcaatgatctat
ctaatcttctagtggttcttaactcgggtacaactttcattatctatttcactttctcag
agaagtacaggcaaactttagtttttattctcaagaatggatgttgtgcaaccgtcagcg
actacaacaactatacagccatgtctagaacggcttcgatgagaatttcaagcgagacgg
gcggtcaaattcaacgacagggatctaaaatgagcaatagcagccgctcatcggacgttc
ttctcaaaccaatttacatgcagaaaagaagcgaacgcttctcgtcggagtacaacgaac
gaacttgcaaacaccttgctcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGGTACTACGTTGGCTACTACAC 59 499 524 Antisense
ACAGAGCACGCGGGGACTGGCATAT 247 107 663 Antisense
ATCTTCTAGTGGTTCTTAACTCGGG 59 19 707 Antisense
AACTCGGGTACAACTTTCATTATCT 528 141 724 Antisense
TTTCATTATCTATTTCACTTTCTCA 267 673 738 Antisense
TGTTGTGCAACCGTCAGCGACTACA 416 563 805 Antisense
CAGCGACTACAACAACTATACAGCC 188 203 819 Antisense
GCCATGTCTAGAACGGCTTCGATGA 249 281 841 Antisense
GACGGGCGGTCAAATTCAACGACAG 686 375 879 Antisense
AACGCTTCTCGTCGGAGTACAACGA 622 145 977 Antisense
ACGAACTTGCAAACACCTTGCTCCT 374 91 1002 Antisense
Sequence Source Affymetrix Proprietary Database