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[181645_at]

Description F58E1.8 /REP_DB=WormBase Gene ID /WP=CE11386 /TR=Q9TZG4 /GB=AAC67477.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 69      4grp_total > 18      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 5.6
(-3)
A 		29.4
(1.9)
A 		18.9
(-0.2)
A 		34.6
(3)
A 		29
B 4
(-2.4)
A 		17.6
(3)
A 		2.5
(-3)
A 		13.8
(1.5)
A 		15
C 16.9
(-1.8)
A 		6
(-2.6)
A 		1.6
(-3)
A 		75.8
(3)
P 		74
avr 8.8
(-2.8) 		17.7
(-1.2) 		7.7
(-3) 		41.4
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 38.5
(-0.1)
A 		11.8
(-2.2)
A 		4.5
(-2.8)
A 		2.3
(-3)
A 		39.5
(-0)
A 		77.2
(3)
P 		75
B 16.9
(-0.1)
A 		9.6
(-2.3)
A 		19.6
(0.7)
A 		7.4
(-3)
A 		17.8
(0.2)
A 		27.1
(3)
P 		20
C 18.6
(-1.8)
A 		29.9
(-0.5)
P 		8
(-3)
A 		31.8
(-0.3)
A 		13.6
(-2.4)
A 		60.1
(3)
P 		52
avr 24.7
(-1.1) 		17.1
(-2.1) 		10.7
(-3) 		13.8
(-2.6) 		23.6
(-1.2) 		54.8
(3) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 2
(-3)
A 		6.4
(3)
A 		4.9
(1)
A 		5.1
(1.2)
A 		4
B 1.3
(-1.9)
A 		4.3
(3)
A 		0.6
(-3)
A 		2.9
(0.7)
A 		4
C 5.6
(-0.5)
A 		1.7
(-2.5)
A 		0.6
(-3)
A 		12.8
(3)
P 		12
avr 3
(-1.8) 		4.1
(-0.4) 		2
(-3) 		6.9
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 7.1
(-0.9)
A 		4.2
(-1.8)
A 		1
(-2.9)
A 		0.6
(-3)
A 		11.9
(0.7)
A 		18.8
(3)
P 		18
B 5.9
(-0.1)
A 		3.2
(-2.1)
A 		4.8
(-0.9)
A 		2
(-3)
A 		7.3
(0.9)
A 		10.1
(3)
P 		8
C 6.9
(-1.4)
A 		9.1
(-0.6)
P 		2
(-3)
A 		6.4
(-1.5)
A 		4.4
(-2.2)
A 		20.1
(3)
P 		18
avr 6.6
(-1.2) 		5.5
(-1.7) 		2.6
(-3) 		3
(-2.8) 		7.9
(-0.7) 		16.3
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181645_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5610
Sequence Type Exemplar sequence
Representative Public ID F58E1.8 NCBI
Target Description F58E1.8 /REP_DB=WormBase Gene ID /WP=CE11386 /TR=Q9TZG4 /GB=AAC67477.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061640(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061640 NCBI Caenorhabditis elegans F58E1.8 (F58E1.8) mRNA, complete cds. 11/11 None
SNAP00000040828 ENSEMBL cdna:SNAP chromosome:CEL140:II:1686837:1687793:-1 11/11 None
F58E1.8 ENSEMBL cdna:known chromosome:CEL140:II:1686080:1687793:-1 gene:F58E1.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040843 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1686078-1687792(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58E1.8
Entrez Gene 186513 Entrez gene
SwissProt Q9TZG4 EMBL-EBI
WormBase CE11386 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67477 Hypothetical protein F58E1.8 [Caenorhabditis elegans] ref|NP_494041.1| F58E1.8 [Caenorhabditis elegans] 1.0E-171
blast AAC67477 Hypothetical protein F58E1.8 [Caenorhabditis elegans] ref|NP_494041.1| F58E1.8 [Caenorhabditis elegans] 1.0E-162
blast AAC67486 Hypothetical protein F58E1.9 [Caenorhabditis elegans] ref|NP_494042.1| F58E1.9 [Caenorhabditis elegans] 1.0E-112
blast AAC67486 Hypothetical protein F58E1.9 [Caenorhabditis elegans] ref|NP_494042.1| F58E1.9 [Caenorhabditis elegans] 1.0E-107
blast CAB60508 Hypothetical protein Y56A3A.14 [Caenorhabditis elegans] ref|NP_499546.1| Y56A3A.14 [Caenorhabditis elegans] 2.0E-43
blast CAB60522 Hypothetical protein Y56A3A.10 [Caenorhabditis elegans] ref|NP_499540.1| Y56A3A.10 [Caenorhabditis elegans] 2.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.0E-5
Sequence
Target Sequence 
>C. ELEGANS:181645_AT
gttgtattaatcagcagtctatcggaaaggaaaatcgaagacatctatgattctattaaa
gaacttgacatagtcgcactgcatatgatctcctctcaatttcaagactgcaatcttctc
aagctgttccctacactagaggaactgaccgtttccgagaatccactgagtgctccaatc
tttgctcagaattttcagagtctcactgcacccaatgtggcatttgacaacatatttttc
tcaaactgcatcaatttggtcttataccatcaagttatatctgataaggatttaaacatc
ttcatgaggaattggattaaaggatccaatccccgtcttcgaaaactcacaaacgcgtca
aatgaacacaacagagtaatagtggaatccgtattttttcaaggaattgcttacattgaa
accagggactattgcgacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGTATTAATCAGCAGTCTATCGG 474 437 379 Antisense
ATAGTCGCACTGCATATGATCTCCT 71 23 448 Antisense
GATCTCCTCTCAATTTCAAGACTGC 80 419 465 Antisense
TCAAGACTGCAATCTTCTCAAGCTG 528 629 480 Antisense
GGAACTGACCGTTTCCGAGAATCCA 700 529 519 Antisense
GAATCCACTGAGTGCTCCAATCTTT 167 331 537 Antisense
GAATTTTCAGAGTCTCACTGCACCC 230 325 567 Antisense
CTCACTGCACCCAATGTGGCATTTG 145 229 580 Antisense
AACTGCATCAATTTGGTCTTATACC 266 143 622 Antisense
CGAAAACTCACAAACGCGTCAAATG 617 251 718 Antisense
ATTGAAACCAGGGACTATTGCGACG 30 3 793 Antisense
Sequence Source Affymetrix Proprietary Database