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[181632_at]

Description B0281.3 /REP_DB=WormBase Gene ID /WP=CE07714 /TR=O16614 /GB=AAB66082.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 71      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 72      6grp_total > 71      4grp_total > 54      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 499
1 2 3 4 range
A 576.3
(3)
P 		267.5
(-1.3)
P 		260.6
(-1.4)
P 		147.1
(-3)
P 		429
B 544.2
(3)
P 		284.8
(-0.7)
P 		335.4
(0)
P 		125.8
(-3)
P 		418
C 625.1
(3)
P 		233.1
(-1.9)
P 		327.2
(-0.7)
P 		143.7
(-3)
P 		481
avr 581.9
(3) 		261.8
(-1.3) 		307.7
(-0.7) 		138.9
(-3) 		443

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 347.2
(-0.8)
P 		405.3
(0.3)
P 		532.7
(2.8)
P 		544.9
(3)
P 		433
(0.9)
P 		229.3
(-3)
P 		316
B 297.4
(-3)
P 		370.7
(-1.5)
P 		536
(1.8)
P 		596.2
(3)
P 		452
(0.1)
P 		328.4
(-2.4)
P 		299
C 312.4
(-3)
P 		370.2
(-1.4)
P 		450.2
(0.9)
P 		526.8
(3)
P 		371.1
(-1.4)
P 		362.1
(-1.6)
P 		214
avr 319
(-2.7) 		382.1
(-1.2) 		506.3
(1.8) 		556
(3) 		418.7
(-0.3) 		306.6
(-3) 		249
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 209.8
(3)
P 		58.1
(-1.8)
P 		68.1
(-1.5)
P 		21.5
(-3)
P 		188
B 173.5
(3)
P 		70.3
(-1.2)
P 		77.7
(-0.9)
P 		26.8
(-3)
P 		147
C 206.9
(3)
P 		67
(-1.6)
P 		114
(-0.1)
P 		24.2
(-3)
P 		183
avr 196.7
(3) 		65.1
(-1.6) 		86.6
(-0.8) 		24.2
(-3) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 64
(-2.4)
P 		144.1
(3)
P 		116
(1.1)
P 		138.2
(2.6)
P 		130.7
(2.1)
P 		55.8
(-3)
P 		88
B 103.5
(-3)
P 		123.4
(-1.5)
P 		130.2
(-1)
P 		157.9
(1.1)
P 		183.6
(3)
P 		122.5
(-1.6)
P 		80
C 115.8
(0.9)
P 		113
(-0.2)
P 		114.6
(0.4)
P 		105.9
(-3)
P 		120.1
(2.6)
P 		121.2
(3)
P 		15
avr 94.4
(-3) 		126.8
(0.9) 		120.3
(0.1) 		134
(1.7) 		144.8
(3) 		99.8
(-2.4) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181632_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5663
Sequence Type Exemplar sequence
Representative Public ID B0281.3 NCBI
Target Description B0281.3 /REP_DB=WormBase Gene ID /WP=CE07714 /TR=O16614 /GB=AAB66082.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061831(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061831 NCBI Caenorhabditis elegans B0281.3 (B0281.3) mRNA, complete cds. 11/11 None
GENEFINDER00000033222 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2307002:2309040:1 11/11 None
B0281.3 ENSEMBL cdna:known chromosome:CEL140:II:2306938:2308177:1 gene:B0281.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033216 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2306936-2308176(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0281.3
Entrez Gene 181897 Entrez gene
SwissProt O16614 EMBL-EBI
WormBase CE07714 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66082 Hypothetical protein B0281.3 [Caenorhabditis elegans] ref|NP_494232.1| B0281.3 [Caenorhabditis elegans] 0.0
blast AAB66082 Hypothetical protein B0281.3 [Caenorhabditis elegans] ref|NP_494232.1| B0281.3 [Caenorhabditis elegans] 1.0E-177
blast AAB66197 Hypothetical protein K09F6.7 [Caenorhabditis elegans] ref|NP_494227.1| K09F6.7 [Caenorhabditis elegans] 5.0E-73
blast AAB66197 Hypothetical protein K09F6.7 [Caenorhabditis elegans] ref|NP_494227.1| K09F6.7 [Caenorhabditis elegans] 7.0E-73
blast AAB66086 Hypothetical protein B0281.8 [Caenorhabditis elegans] ref|NP_494234.1| B0281.8 [Caenorhabditis elegans] 6.0E-62
blast AAB66086 Hypothetical protein B0281.8 [Caenorhabditis elegans] ref|NP_494234.1| B0281.8 [Caenorhabditis elegans] 7.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR001841 EMBL-EBI Zn-finger, RING 8.7E-6
Pfam IPR001841 EMBL-EBI Zn-finger, RING 8.7E-6
Sequence
Target Sequence 
>C. ELEGANS:181632_AT
cacccgtataatctggctgaatttttctgtgtgaaccccgactgttcctctaacgacaag
ttgatgtgccgcacctgtgaagactttggtgttcataaaggacatgcaaaatatctattg
ataacgagaatagaaaactcagggaattcgcttcagaaagcaataagcgatttgcaaaaa
aaactccgcgacattgaccaaaacgtggaagaattaaaaaaggcacaaacattattcaat
gaaaatggagaactctttcaaaaaaagaaaaaccagataaccaatttttattccaaattg
cgagagaaagtcaatgaacgagaaaaagaagcgctgtccgaattgaaggatgtagcacgg
accgccagggtaaataatacaaagtctctactgactttgatgcaagttcgcagtatatac
gatgaactcatcagtgaagcgaaagaacttttgaaaaagactagcgcaacttcatttcgt
gaagttgaagccattctgaatagatcgaatgctcacataatgtcacaagaaaaaattttt
gagccaaatccattcgagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCCGTATAATCTGGCTGAATTTT 56 201 379 Antisense
TGAATTTTTCTGTGTGAACCCCGAC 506 529 396 Antisense
CGACTGTTCCTCTAACGACAAGTTG 312 251 417 Antisense
CGACAAGTTGATGTGCCGCACCTGT 173 253 432 Antisense
GAAAACTCAGGGAATTCGCTTCAGA 470 353 511 Antisense
AACTCCGCGACATTGACCAAAACGT 79 143 560 Antisense
AAAGAAGCGCTGTCCGAATTGAAGG 276 115 703 Antisense
ATGTAGCACGGACCGCCAGGGTAAA 647 45 728 Antisense
AGACTAGCGCAACTTCATTTCGTGA 318 71 836 Antisense
GATCGAATGCTCACATAATGTCACA 571 417 881 Antisense
TTTTTGAGCCAAATCCATTCGAGAA 382 675 914 Antisense
Sequence Source Affymetrix Proprietary Database