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[181627_at]

Description F09E5.5 /REP_DB=WormBase Gene ID /WP=CE27940 /TR=Q19262 /GB=AAA79344.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 0      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 41      6grp_total > 08      4grp_total > 54      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 482
1 2 3 4 range
A 775.5
(3)
P 		520.3
(-1.2)
P 		540.4
(-0.8)
P 		407.6
(-3)
P 		368
B 710.4
(3)
P 		582.2
(1.2)
P 		393.1
(-1.6)
P 		293.2
(-3)
P 		417
C 715.7
(3)
P 		480.6
(-1.1)
P 		486
(-1)
P 		369.1
(-3)
P 		347
avr 733.9
(3) 		527.7
(-0.3) 		473.2
(-1.1) 		356.6
(-3) 		377

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 376
1 2 3 4 5 6 range
A 860.3
(3)
P 		762.3
(1.3)
P 		703.5
(0.3)
P 		840.8
(2.7)
P 		507.2
(-3)
P 		589.9
(-1.6)
P 		353
B 866
(3)
P 		615.4
(-1)
P 		489.6
(-3)
P 		659
(-0.3)
P 		556.6
(-1.9)
P 		662
(-0.3)
P 		376
C 713.5
(2.1)
P 		668.6
(0.9)
P 		747.6
(3)
P 		626.3
(-0.2)
P 		539
(-2.5)
P 		520.1
(-3)
P 		228
avr 813.3
(3) 		682.1
(0.2) 		646.9
(-0.6) 		708.7
(0.8) 		534.3
(-3) 		590.7
(-1.8) 		279
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 282.3
(3)
P 		112.9
(-1.6)
P 		141.3
(-0.8)
P 		59.7
(-3)
P 		223
B 226.5
(3)
P 		143.8
(-0)
P 		91.1
(-2)
P 		62.5
(-3)
P 		164
C 236.9
(3)
P 		138.2
(-0.4)
P 		169.4
(0.7)
P 		62.2
(-3)
P 		175
avr 248.6
(3) 		131.6
(-0.8) 		133.9
(-0.7) 		61.5
(-3) 		187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 158.6
(-2.3)
P 		271.1
(3)
P 		153.2
(-2.5)
P 		213.2
(0.3)
P 		153
(-2.6)
P 		143.6
(-3)
P 		128
B 301.3
(3)
P 		204.9
(-0.2)
P 		118.9
(-3)
P 		174.5
(-1.2)
P 		226.1
(0.5)
P 		246.9
(1.2)
P 		182
C 264.5
(3)
P 		204
(0.4)
P 		190.3
(-0.2)
P 		126
(-3)
P 		174.4
(-0.9)
P 		174.1
(-0.9)
P 		139
avr 241.5
(3) 		226.7
(2) 		154.1
(-3) 		171.2
(-1.8) 		184.5
(-0.9) 		188.2
(-0.7) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181627_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4794
Sequence Type Exemplar sequence
Representative Public ID F09E5.5 NCBI
Target Description F09E5.5 /REP_DB=WormBase Gene ID /WP=CE27940 /TR=Q19262 /GB=AAA79344.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062605 NCBI Caenorhabditis elegans F09E5.5 (F09E5.5) mRNA, complete cds. 11/11 A 
SNAP00000044016 ENSEMBL cdna:SNAP chromosome:CEL140:II:5367842:5371943:1 11/11 None
GENEFINDER00000044027 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5369247:5371943:1 11/11 None
F09E5.5.2 ENSEMBL cdna:known chromosome:CEL140:II:5359522:5372041:1 gene:F09E5.5 11/11 None
F09E5.5.1 ENSEMBL cdna:known chromosome:CEL140:II:5359530:5372092:1 gene:F09E5.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5367920-5371941(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F09E5.5
Entrez Gene 173910 Entrez gene
SwissProt Q19262 EMBL-EBI
WormBase CE29752 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261520_AT expressed protein at
CANINE:1604179_AT similar to SEC6-like 1 cfa
CANINE_2:CFA.19769.1.S1_S_AT similar to SEC6-like 1 cfa
CANINE_2:CFAAFFX.17062.1.S1_AT similar to SEC6-like 1 cfa
DROSOPHILA_2:1638795_AT dm
DROSGENOME1:154282_AT dm
CHICKEN:GGA.1387.1.S1_S_AT SEC6-like 1 (S. cerevisiae) gga
HU35KSUBA:R56174_AT SEC6-like 1 (S. cerevisiae) hs
HU35KSUBB:RC_T33892_AT SEC6-like 1 (S. cerevisiae) hs
HU35KSUBB:RC_F02418_AT SEC6-like 1 (S. cerevisiae) hs
HU35KSUBD:RC_AA487875_S_AT SEC6-like 1 (S. cerevisiae) hs
U133_X3P:HS.8088.0.A1_3P_AT SEC6-like 1 (S. cerevisiae) hs
U133_X3P:HS.107614.1.A1_3P_AT SEC6-like 1 (S. cerevisiae) hs
HG-U133A_2:212630_AT SEC6-like 1 (S. cerevisiae) hs
HG-U133_PLUS_2:212630_AT SEC6-like 1 (S. cerevisiae) hs
HG-U133A:212630_AT SEC6-like 1 (S. cerevisiae) hs
HG-U133_PLUS_2:228847_AT SEC6-like 1 (S. cerevisiae) hs
HG-U133B:228847_AT SEC6-like 1 (S. cerevisiae) hs
HG-U95AV2:37597_S_AT SEC6-like 1 (S. cerevisiae) hs
HG-U95B:49660_AT SEC6-like 1 (S. cerevisiae) hs
HU35KSUBC:RC_W60995_AT SEC6-like 1 (S. cerevisiae) hs
MG-U74BV2:113161_AT SEC6-like 1 (S. cerevisiae) mm
MOE430B:1434750_AT SEC6-like 1 (S. cerevisiae) mm
MOUSE430_2:1434750_AT SEC6-like 1 (S. cerevisiae) mm
MU19KSUBB:TC30504_AT SEC6-like 1 (S. cerevisiae), mRNA (cDNA clone MGC:28499 IMAGE:4187527) mm
MU19KSUBB:TC34027_AT SEC6-like 1 (S. cerevisiae), mRNA (cDNA clone MGC:28499 IMAGE:4187527) mm
MU19KSUBC:TC38147_AT SEC6-like 1 (S. cerevisiae), mRNA (cDNA clone MGC:28499 IMAGE:4187527) mm
RAT230_2:1370686_AT SEC6-like 1 (S. cerevisiae) rn
RAE230A:1370686_AT SEC6-like 1 (S. cerevisiae) rn
RG-U34A:U32575_AT SEC6-like 1 (S. cerevisiae) rn
RG-U34A:U32575_G_AT SEC6-like 1 (S. cerevisiae) rn
RG-U34B:RC_AI045764_AT SEC6-like 1 (S. cerevisiae) rn
RG-U34B:RC_AA851443_S_AT SEC6-like 1 (S. cerevisiae) rn
RAE230A:1389624_S_AT SEC6-like 1 (S. cerevisiae) rn
RAT230_2:1389624_S_AT SEC6-like 1 (S. cerevisiae) rn
RAE230A:1374656_AT SEC6-like 1 (S. cerevisiae) rn
RAT230_2:1374656_AT SEC6-like 1 (S. cerevisiae) rn
YEAST_2:1776607_AT Essential 88kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis Sc
Protein Similarities
Method ID Description E-Value
blast AAA79344 Hypothetical protein F09E5.5 [Caenorhabditis elegans] ref|NP_495006.2| F09E5.5 [Caenorhabditis elegans] sp|Q19262|SEC6_CAEEL Proable exocyst complex component Sec6 0.0
blast CAE59085 Hypothetical protein CBG02377 [Caenorhabditis briggsae] 0.0
blast T16000 hypothetical protein F09E5.6 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010326 EMBL-EBI Exocyst complex component Sec6 1.0E-126
Pfam IPR010326 EMBL-EBI Exocyst complex component Sec6 2.1E-9
Pfam IPR010326 EMBL-EBI Exocyst complex component Sec6 1.9E-87
Pfam IPR010326 EMBL-EBI Exocyst complex component Sec6 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:181627_AT
ttaaccggaagtgctgctctcgagacagtttgtatgactatttccgattattaccacgat
cacaagcatctgcgaccagtcacaaggtccactttgcttatggatcttcaatttagaatt
gttagcgaatatttgaaggcaatcgaaacaaagcgagtgagtctcaattcgtatgaagag
cgtgcacttgcaggaaagagaatgaaagctgatgttgttcgtttggataatctttatgct
gaatttgcgacaagttctgatatggctgatcagctcccactcctaacatcaattgtggct
gcagctggagatgtgatttctctaaaagacaaatctcttctgtcacttgaagccacttca
tttgctcggaaatttccaaattgtcctgctgaattgctttctgctgttattgcgactcgt
gatgatattagtggcagtgaagctcgtagtttggctagtgaagttctacaacatgtacag
ttccatccaaaggatcagatattcgatcaactttttgctttgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTAACCGGAAGTGCTGCTCTCGAGA 237 683 616 Antisense
GACTATTTCCGATTATTACCACGAT 557 371 651 Antisense
TACCACGATCACAAGCATCTGCGAC 405 651 667 Antisense
ACCAGTCACAAGGTCCACTTTGCTT 656 85 690 Antisense
TGATATGGCTGATCAGCTCCCACTC 170 565 873 Antisense
AACATCAATTGTGGCTGCAGCTGGA 200 137 900 Antisense
TGAAGCCACTTCATTTGCTCGGAAA 509 573 963 Antisense
TCCTGCTGAATTGCTTTCTGCTGTT 86 593 999 Antisense
TTCTGCTGTTATTGCGACTCGTGAT 512 691 1014 Antisense
GTGGCAGTGAAGCTCGTAGTTTGGC 474 489 1046 Antisense
TATTCGATCAACTTTTTGCTTTGCG 24 665 1115 Antisense
Sequence Source Affymetrix Proprietary Database